{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/35558"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/35558","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"The Effects of Temperature On The Durability Of Resistance Of Soybean To Soybean Mosaic Virus","abstract":"The objectives of this study were to determine the effects the temperature sensitivity of alleles of Rsv1 in soybean (Glycine max (L.) Merr.). Soybean cultivars carrying alleles of Rsv were exposed to 1 several heat treatments designed to induce heat shock protein production prior to inoculation with soybean mosaic virus (SMV). The heat treatment methods were similar to those employed in the research with N gene-tobacco mosaic virus studies. The soybean cultivars used were Lee 69, York, Kwanggyo, Ogden and PI96983, carrying the Rsv, Rsv1-y, Rsv1-k, Rsv1-t, and Rsv1 allles of Rsv1, respectively, and were selected to provide a range of reactions to selected SMV pathotype groups. For example Rsv1-y and Rsv1-k give a necrotic response to SMV G4 and SMV G6, respectively, while both are resistant to SMV G1. To determine the durability of resistance under heat shock conditions, the symptoms were observed for changes in the phenotype of the resistance response. Immunological techniques were employed to determine the vascular movement and localization of the viral antigen in the plant. Heat treatments used were found to induce HSP but to have no effect on the resistance phenotype. A detached leaf assay was used to test the same Rsv alleles at constant 1 high temperatures. Primary trifoliolate leaflets were removed and inoculated, then placed into a continuously lighted incubator at 20 °C or 30 °C. Leaf immunoprint assays were used to determine the localization of the viral antigen. The visible symptoms for necrotic lesions and veins were observed for necrotic phenotype-pathotype combinations but mosaic symptoms were not observed on detached leaves, as expected for inoculated leaves. The detached leaf assay confirmed that no change from the expected resistance response of the Rsv alleles occurred at 30 C. A breakdown 1 o of resistance to SMV at high temperature had been reported in soybean by Tu and Buzzell (1987). The resistance gene in which the high temperature breakdown occurred has been determined to be Rsv . Using cultivars and breeding lines carrying Rsv a similar experiment was attempted in growth 3 3 chambers. Preliminary results suggest that Rsv is temperature sensitive.","abstract_html":"The objectives of this study were to determine the effects the temperature sensitivity of alleles of Rsv1 in soybean (Glycine max (L.) Merr.). Soybean cultivars carrying alleles of Rsv were exposed to 1 several heat treatments designed to induce heat shock protein production prior to inoculation with soybean mosaic virus (SMV). The heat treatment methods were similar to those employed in the research with N gene-tobacco mosaic virus studies. The soybean cultivars used were Lee 69, York, Kwanggyo, Ogden and PI96983, carrying the Rsv, Rsv1-y, Rsv1-k, Rsv1-t, and Rsv1 allles of Rsv1, respectively, and were selected to provide a range of reactions to selected SMV pathotype groups. For example Rsv1-y and Rsv1-k give a necrotic response to SMV G4 and SMV G6, respectively, while both are resistant to SMV G1. To determine the durability of resistance under heat shock conditions, the symptoms were observed for changes in the phenotype of the resistance response. Immunological techniques were employed to determine the vascular movement and localization of the viral antigen in the plant. Heat treatments used were found to induce HSP but to have no effect on the resistance phenotype. A detached leaf assay was used to test the same Rsv alleles at constant 1 high temperatures. Primary trifoliolate leaflets were removed and inoculated, then placed into a continuously lighted incubator at 20 °C or 30 °C. Leaf immunoprint assays were used to determine the localization of the viral antigen. The visible symptoms for necrotic lesions and veins were observed for necrotic phenotype-pathotype combinations but mosaic symptoms were not observed on detached leaves, as expected for inoculated leaves. The detached leaf assay confirmed that no change from the expected resistance response of the Rsv alleles occurred at 30 C. A breakdown 1 o of resistance to SMV at high temperature had been reported in soybean by Tu and Buzzell (1987). The resistance gene in which the high temperature breakdown occurred has been determined to be Rsv . Using cultivars and breeding lines carrying Rsv a similar experiment was attempted in growth 3 3 chambers. Preliminary results suggest that Rsv is temperature sensitive.","abstract_has_math":false,"creators":["Flora, Jonathan P."],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Plant Pathology, Physiology, and Weed Science","degree_department":"Plant Pathology, Physiology, and Weed Science","school":null,"contributors":[],"advisors":[],"committee_chairs":["Tolin, Sue A."],"committee_members":["Buss, Glenn R.","Warren, Herman L."],"year":1997,"date_issued":"1997-05-08","date_published":"1997-05-08","updated_at":"2026-07-22T22:19:15Z","subjects":["soybean mosaic virus","resistance","heat shock"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-10623859701581"],"render_values":[{"text":"etd-10623859701581","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/35558","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Tolin, Sue A."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Buss, Glenn R.","Warren, Herman L."]},{"key":"dc:contributor.department","label":"Department","values":["Plant Pathology, Physiology, and Weed Science"]},{"key":"dc:creator","label":"Author","values":["Flora, Jonathan P."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T20:47:20Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T20:47:20Z","1998-05-08"]},{"key":"dc:date.issued","label":"Date","values":["1997-05-08"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Pathology, Physiology, and Weed Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["soybean mosaic virus","resistance","heat shock"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-10623859701581"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/35558"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The objectives of this study were to determine the effects the temperature sensitivity of alleles of Rsv1 in soybean (Glycine max (L.) Merr.). Soybean cultivars carrying alleles of Rsv were exposed to 1 several heat treatments designed to induce heat shock protein production prior to inoculation with soybean mosaic virus (SMV). The heat treatment methods were similar to those employed in the research with N gene-tobacco mosaic virus studies. The soybean cultivars used were Lee 69, York, Kwanggyo, Ogden and PI96983, carrying the Rsv, Rsv1-y, Rsv1-k, Rsv1-t, and Rsv1 allles of Rsv1, respectively, and were selected to provide a range of reactions to selected SMV pathotype groups. For example Rsv1-y and Rsv1-k give a necrotic response to SMV G4 and SMV G6, respectively, while both are resistant to SMV G1. To determine the durability of resistance under heat shock conditions, the symptoms were observed for changes in the phenotype of the resistance response. Immunological techniques were employed to determine the vascular movement and localization of the viral antigen in the plant. Heat treatments used were found to induce HSP but to have no effect on the resistance phenotype. A detached leaf assay was used to test the same Rsv alleles at constant 1 high temperatures. Primary trifoliolate leaflets were removed and inoculated, then placed into a continuously lighted incubator at 20 °C or 30 °C. Leaf immunoprint assays were used to determine the localization of the viral antigen. The visible symptoms for necrotic lesions and veins were observed for necrotic phenotype-pathotype combinations but mosaic symptoms were not observed on detached leaves, as expected for inoculated leaves. The detached leaf assay confirmed that no change from the expected resistance response of the Rsv alleles occurred at 30 C. A breakdown 1 o of resistance to SMV at high temperature had been reported in soybean by Tu and Buzzell (1987). The resistance gene in which the high temperature breakdown occurred has been determined to be Rsv . Using cultivars and breeding lines carrying Rsv a similar experiment was attempted in growth 3 3 chambers. Preliminary results suggest that Rsv is temperature sensitive."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:title","label":"Title","values":["The Effects of Temperature On The Durability Of Resistance Of Soybean To Soybean Mosaic Virus"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Tolin, Sue A."],"dc:contributor.committeemember":["Buss, Glenn R.","Warren, Herman L."],"dc:contributor.department":["Plant Pathology, Physiology, and Weed Science"],"dc:creator":["Flora, Jonathan P."],"dc:date.accessioned":["2014-03-14T20:47:20Z"],"dc:date.available":["2014-03-14T20:47:20Z","1998-05-08"],"dc:date.issued":["1997-05-08"],"dc:description.abstract":["The objectives of this study were to determine the effects the temperature sensitivity of alleles of Rsv1 in soybean (Glycine max (L.) Merr.). Soybean cultivars carrying alleles of Rsv were exposed to 1 several heat treatments designed to induce heat shock protein production prior to inoculation with soybean mosaic virus (SMV). The heat treatment methods were similar to those employed in the research with N gene-tobacco mosaic virus studies. The soybean cultivars used were Lee 69, York, Kwanggyo, Ogden and PI96983, carrying the Rsv, Rsv1-y, Rsv1-k, Rsv1-t, and Rsv1 allles of Rsv1, respectively, and were selected to provide a range of reactions to selected SMV pathotype groups. For example Rsv1-y and Rsv1-k give a necrotic response to SMV G4 and SMV G6, respectively, while both are resistant to SMV G1. To determine the durability of resistance under heat shock conditions, the symptoms were observed for changes in the phenotype of the resistance response. Immunological techniques were employed to determine the vascular movement and localization of the viral antigen in the plant. Heat treatments used were found to induce HSP but to have no effect on the resistance phenotype. A detached leaf assay was used to test the same Rsv alleles at constant 1 high temperatures. Primary trifoliolate leaflets were removed and inoculated, then placed into a continuously lighted incubator at 20 °C or 30 °C. Leaf immunoprint assays were used to determine the localization of the viral antigen. The visible symptoms for necrotic lesions and veins were observed for necrotic phenotype-pathotype combinations but mosaic symptoms were not observed on detached leaves, as expected for inoculated leaves. The detached leaf assay confirmed that no change from the expected resistance response of the Rsv alleles occurred at 30 C. A breakdown 1 o of resistance to SMV at high temperature had been reported in soybean by Tu and Buzzell (1987). The resistance gene in which the high temperature breakdown occurred has been determined to be Rsv . Using cultivars and breeding lines carrying Rsv a similar experiment was attempted in growth 3 3 chambers. Preliminary results suggest that Rsv is temperature sensitive."],"dc:description.degree":["Master of Science"],"dc:identifier.other":["etd-10623859701581"],"dc:identifier.uri":["http://hdl.handle.net/10919/35558"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["soybean mosaic virus","resistance","heat shock"],"dc:title":["The Effects of Temperature On The Durability Of Resistance Of Soybean To Soybean Mosaic Virus"],"dc:type":["Thesis"],"thesis:degree_discipline":["Plant Pathology, Physiology, and Weed Science"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:15Z"}