{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/34947"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/34947","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Identification of juvenile hormone response genes in newly emerged female Aedes aegypti","abstract":"Juvenile hormone (JH) plays pivotal roles in the development and reproduction of insects. Efforts to characterize the mechanisms of JH regulation are complicated due to JH pathways often being intertwined with those of 20-hydroxyecdysone (20E). Upon adult emergence, female Aedes aegypti enter a period of development during which they gain competence for mating, bloodfeeding, and egg production. JH levels rise dramatically and peak during the first 2-3 days post-emergence and remain relatively high until a bloodmeal is consumed, while 20E titers remain very low throughout the entire stage. Thus, post-emergence development offers a unique opportunity to study the effects of JH in the absence of 20E. In this study, four potential JH response genes were identified in newly emerged females. One such gene, AaKr-h1, is a homologue of Kr-h1, a zinc-finger transcription factor which has been characterized in Manduca sexta, Drosophila melanogaster, Tribolium castaneum, and Apis mellifera, and is involved in a diverse range of JH-regulated pathways. AaKr-h1 demonstrated a dose-dependent transcriptional response to JHIII as well as two JH mimics in abdominal ligation assays. The findings of this study indicate that Kr-h1 may be regulated by JH independently of any 20E regulation and suggests a fundamental, conserved role for Kr-h1 in JH-regulated pathways.","abstract_html":"Juvenile hormone (JH) plays pivotal roles in the development and reproduction of insects. Efforts to characterize the mechanisms of JH regulation are complicated due to JH pathways often being intertwined with those of 20-hydroxyecdysone (20E). Upon adult emergence, female Aedes aegypti enter a period of development during which they gain competence for mating, bloodfeeding, and egg production. JH levels rise dramatically and peak during the first 2-3 days post-emergence and remain relatively high until a bloodmeal is consumed, while 20E titers remain very low throughout the entire stage. Thus, post-emergence development offers a unique opportunity to study the effects of JH in the absence of 20E. In this study, four potential JH response genes were identified in newly emerged females. One such gene, AaKr-h1, is a homologue of Kr-h1, a zinc-finger transcription factor which has been characterized in Manduca sexta, Drosophila melanogaster, Tribolium castaneum, and Apis mellifera, and is involved in a diverse range of JH-regulated pathways. AaKr-h1 demonstrated a dose-dependent transcriptional response to JHIII as well as two JH mimics in abdominal ligation assays. The findings of this study indicate that Kr-h1 may be regulated by JH independently of any 20E regulation and suggests a fundamental, conserved role for Kr-h1 in JH-regulated pathways.","abstract_has_math":false,"creators":["Busche, Jefferson M."],"institution":"Virginia Tech","degree_name":"Master of Science in Life Sciences","degree_level":"masters","degree_discipline":"Biochemistry","degree_department":"Biochemistry","school":null,"contributors":[],"advisors":[],"committee_chairs":["Zhu, Jinsong"],"committee_members":["Tu, Zhijian Jake","Gillaspy, Glenda E."],"year":2009,"date_issued":"2009-08-19","date_published":"2009-08-19","updated_at":"2026-07-22T22:19:16Z","subjects":["juvenile hormone","Kr-h1","Aedes aegypti"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-09062009-181120"],"render_values":[{"text":"etd-09062009-181120","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/34947","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Zhu, Jinsong"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Tu, Zhijian Jake","Gillaspy, Glenda E."]},{"key":"dc:contributor.department","label":"Department","values":["Biochemistry"]},{"key":"dc:creator","label":"Author","values":["Busche, Jefferson M."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T20:44:52Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T20:44:52Z","2012-05-08"]},{"key":"dc:date.issued","label":"Date","values":["2009-08-19"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Life Sciences"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["juvenile hormone","Kr-h1","Aedes aegypti"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-09062009-181120"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/34947"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Juvenile hormone (JH) plays pivotal roles in the development and reproduction of insects. Efforts to characterize the mechanisms of JH regulation are complicated due to JH pathways often being intertwined with those of 20-hydroxyecdysone (20E). Upon adult emergence, female Aedes aegypti enter a period of development during which they gain competence for mating, bloodfeeding, and egg production. JH levels rise dramatically and peak during the first 2-3 days post-emergence and remain relatively high until a bloodmeal is consumed, while 20E titers remain very low throughout the entire stage. Thus, post-emergence development offers a unique opportunity to study the effects of JH in the absence of 20E. In this study, four potential JH response genes were identified in newly emerged females. One such gene, AaKr-h1, is a homologue of Kr-h1, a zinc-finger transcription factor which has been characterized in Manduca sexta, Drosophila melanogaster, Tribolium castaneum, and Apis mellifera, and is involved in a diverse range of JH-regulated pathways. AaKr-h1 demonstrated a dose-dependent transcriptional response to JHIII as well as two JH mimics in abdominal ligation assays. The findings of this study indicate that Kr-h1 may be regulated by JH independently of any 20E regulation and suggests a fundamental, conserved role for Kr-h1 in JH-regulated pathways."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science in Life Sciences"]},{"key":"dc:title","label":"Title","values":["Identification of juvenile hormone response genes in newly emerged female Aedes aegypti"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Zhu, Jinsong"],"dc:contributor.committeemember":["Tu, Zhijian Jake","Gillaspy, Glenda E."],"dc:contributor.department":["Biochemistry"],"dc:creator":["Busche, Jefferson M."],"dc:date.accessioned":["2014-03-14T20:44:52Z"],"dc:date.available":["2014-03-14T20:44:52Z","2012-05-08"],"dc:date.issued":["2009-08-19"],"dc:description.abstract":["Juvenile hormone (JH) plays pivotal roles in the development and reproduction of insects. Efforts to characterize the mechanisms of JH regulation are complicated due to JH pathways often being intertwined with those of 20-hydroxyecdysone (20E). Upon adult emergence, female Aedes aegypti enter a period of development during which they gain competence for mating, bloodfeeding, and egg production. JH levels rise dramatically and peak during the first 2-3 days post-emergence and remain relatively high until a bloodmeal is consumed, while 20E titers remain very low throughout the entire stage. Thus, post-emergence development offers a unique opportunity to study the effects of JH in the absence of 20E. In this study, four potential JH response genes were identified in newly emerged females. One such gene, AaKr-h1, is a homologue of Kr-h1, a zinc-finger transcription factor which has been characterized in Manduca sexta, Drosophila melanogaster, Tribolium castaneum, and Apis mellifera, and is involved in a diverse range of JH-regulated pathways. AaKr-h1 demonstrated a dose-dependent transcriptional response to JHIII as well as two JH mimics in abdominal ligation assays. The findings of this study indicate that Kr-h1 may be regulated by JH independently of any 20E regulation and suggests a fundamental, conserved role for Kr-h1 in JH-regulated pathways."],"dc:description.degree":["Master of Science in Life Sciences"],"dc:identifier.other":["etd-09062009-181120"],"dc:identifier.uri":["http://hdl.handle.net/10919/34947"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["juvenile hormone","Kr-h1","Aedes aegypti"],"dc:title":["Identification of juvenile hormone response genes in newly emerged female Aedes aegypti"],"dc:type":["Thesis"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science in Life Sciences"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:16Z"}