{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/33431"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/33431","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Vibrio spp. disinfection and immunization of Cobia (Rachycentron canadum) for the prevention of disease in aquaculture facilities","abstract":"Cobia (Rachycentron canadum) is a tropical marine fish, with increasing commercial aquaculture importance worldwide. One of the major limitations to intensive aquaculture is disease. Diseases spread rapidly in an aquatic environment and pose a major threat to the development and introduction of new species, such as cobia, in aquaculture. This is due to the necessity to use wild caught broodstock, which pose a greater threat to introducing disease to a facility. Bacteria of the genus Vibrio play a major role in the diseases of cultured cobia and other species of marine fish. The goal of this study is to reduce the incidence of disease in a population, by either eliminating the potential pathogen or increasing the resistance of the host. To reach that goal, a disinfection assay to evaluate the effectiveness of nine common aquaculture chemical disinfecting compounds was evaluated against two bacterial pathogens (Vibrio anguillarum and V. ordalii). Both bacterial species were susceptible to a variety of common disinfecting compounds including Chloramine-T®, chlorine, ethanol, iodine, Lysol®, Roccal®-D Plus, and Virkon-S®. In addition, both species showed a resistance to disinfection with formalin and tap water. The humoral immune response of cobia to vaccination with a commercially-available vaccine for Vibrio spp. was evaluated by an ELISA. There was a significant difference between control and vaccinated groups (P<0.0001), showing significant antibody production resulting from vaccination.","abstract_html":"Cobia (Rachycentron canadum) is a tropical marine fish, with increasing commercial aquaculture importance worldwide. One of the major limitations to intensive aquaculture is disease. Diseases spread rapidly in an aquatic environment and pose a major threat to the development and introduction of new species, such as cobia, in aquaculture. This is due to the necessity to use wild caught broodstock, which pose a greater threat to introducing disease to a facility. Bacteria of the genus Vibrio play a major role in the diseases of cultured cobia and other species of marine fish. The goal of this study is to reduce the incidence of disease in a population, by either eliminating the potential pathogen or increasing the resistance of the host. To reach that goal, a disinfection assay to evaluate the effectiveness of nine common aquaculture chemical disinfecting compounds was evaluated against two bacterial pathogens (Vibrio anguillarum and V. ordalii). Both bacterial species were susceptible to a variety of common disinfecting compounds including Chloramine-T®, chlorine, ethanol, iodine, Lysol®, Roccal®-D Plus, and Virkon-S®. In addition, both species showed a resistance to disinfection with formalin and tap water. The humoral immune response of cobia to vaccination with a commercially-available vaccine for Vibrio spp. was evaluated by an ELISA. There was a significant difference between control and vaccinated groups (P&lt;0.0001), showing significant antibody production resulting from vaccination.","abstract_has_math":false,"creators":["Machen, John Wesley"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Veterinary Medical Sciences","degree_department":"Veterinary Medical Sciences","school":null,"contributors":[],"advisors":[],"committee_chairs":["Smith, Stephen A."],"committee_members":["Flick, George J. Jr.","Pierson, Frank William"],"year":2008,"date_issued":"2008-05-22","date_published":"2008-05-22","updated_at":"2026-07-22T22:20:12Z","subjects":["Rachycentron canadum","Cobia","Striped Bass","Morone","Vibrio","disinfection","disease","immunity","vaccination"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-06052008-100915"],"render_values":[{"text":"etd-06052008-100915","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/33431","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Smith, Stephen A."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Flick, George J. 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One of the major limitations to intensive aquaculture is disease. Diseases spread rapidly in an aquatic environment and pose a major threat to the development and introduction of new species, such as cobia, in aquaculture. This is due to the necessity to use wild caught broodstock, which pose a greater threat to introducing disease to a facility. Bacteria of the genus Vibrio play a major role in the diseases of cultured cobia and other species of marine fish. The goal of this study is to reduce the incidence of disease in a population, by either eliminating the potential pathogen or increasing the resistance of the host. To reach that goal, a disinfection assay to evaluate the effectiveness of nine common aquaculture chemical disinfecting compounds was evaluated against two bacterial pathogens (Vibrio anguillarum and V. ordalii). Both bacterial species were susceptible to a variety of common disinfecting compounds including Chloramine-T®, chlorine, ethanol, iodine, Lysol®, Roccal®-D Plus, and Virkon-S®. In addition, both species showed a resistance to disinfection with formalin and tap water. The humoral immune response of cobia to vaccination with a commercially-available vaccine for Vibrio spp. was evaluated by an ELISA. There was a significant difference between control and vaccinated groups (P<0.0001), showing significant antibody production resulting from vaccination."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:title","label":"Title","values":["Vibrio spp. disinfection and immunization of Cobia (Rachycentron canadum) for the prevention of disease in aquaculture facilities"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Smith, Stephen A."],"dc:contributor.committeemember":["Flick, George J. Jr.","Pierson, Frank William"],"dc:contributor.department":["Veterinary Medical Sciences"],"dc:creator":["Machen, John Wesley"],"dc:date.accessioned":["2014-03-14T20:39:25Z"],"dc:date.available":["2014-03-14T20:39:25Z","2010-11-22"],"dc:date.issued":["2008-05-22"],"dc:description.abstract":["Cobia (Rachycentron canadum) is a tropical marine fish, with increasing commercial aquaculture importance worldwide. One of the major limitations to intensive aquaculture is disease. Diseases spread rapidly in an aquatic environment and pose a major threat to the development and introduction of new species, such as cobia, in aquaculture. This is due to the necessity to use wild caught broodstock, which pose a greater threat to introducing disease to a facility. Bacteria of the genus Vibrio play a major role in the diseases of cultured cobia and other species of marine fish. The goal of this study is to reduce the incidence of disease in a population, by either eliminating the potential pathogen or increasing the resistance of the host. To reach that goal, a disinfection assay to evaluate the effectiveness of nine common aquaculture chemical disinfecting compounds was evaluated against two bacterial pathogens (Vibrio anguillarum and V. ordalii). Both bacterial species were susceptible to a variety of common disinfecting compounds including Chloramine-T®, chlorine, ethanol, iodine, Lysol®, Roccal®-D Plus, and Virkon-S®. In addition, both species showed a resistance to disinfection with formalin and tap water. The humoral immune response of cobia to vaccination with a commercially-available vaccine for Vibrio spp. was evaluated by an ELISA. There was a significant difference between control and vaccinated groups (P<0.0001), showing significant antibody production resulting from vaccination."],"dc:description.degree":["Master of Science"],"dc:identifier.other":["etd-06052008-100915"],"dc:identifier.uri":["http://hdl.handle.net/10919/33431"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["Rachycentron canadum","Cobia","Striped Bass","Morone","Vibrio","disinfection","disease","immunity","vaccination"],"dc:title":["Vibrio spp. disinfection and immunization of Cobia (Rachycentron canadum) for the prevention of disease in aquaculture facilities"],"dc:type":["Thesis"],"thesis:degree_discipline":["Veterinary Medical Sciences"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:12Z"}