Microbial Ecology of Acanthamoeba polyphaga and Sulfate-Reducing Bacteria in Premise Plumbing

Great advances have been made in the last 100 years in the effort to provide safe and reliable potable water. Unfortunately, organisms surviving the water treatment process still cause illnesses in the population. Acanthamoebae are ubiquitous in the environment and are resistant to commonly used disinfection methods. In addition to being pathogenic on their own, Acanthamoeba spp. are capable of acting as a host to pathogenic bacteria in potable water. The amoebae provide the bacteria with protection from chemical and physical means of disinfection. In this way many pathogens that would otherwise be killed in the water treatment process survive and are capable of infecting water customers. Most likely due to experimental limitations discussed herein, the concentration of organic carbon in solution was not found to affect the number of Acanthamoeba polyphaga surviving within reactors designed to model residential water tanks. A copper ion concentration of 1.3 mg/L was determined to be an effective disinfectant against A. polyphaga trophozoites, while free chlorine at 10 mg/L and monochloramine at 50 mg/L were deemed effective against trophozoites. Sulfate-reducing bacteria (SRB) are suspected to be causative agents in copper pitting corrosion. SRB have been found in tubercles covering pits in many homes experiencing pinhole leaks, but the mechanisms of the survival of these organisms in potable water systems are poorly understood. Nutrient studies conducted show that the absence of nitrogen in solution may encourage copper corrosion by SRB. In addition, a medium specifically designed to encourage SRB growth resulted in a large increase in copper corrosion as compared to the control water.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Civil Engineering

Department dc:contributor.department

Civil Engineering

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2009

Author dc:creator

• Methvin, Rachel M.

Chair dc:contributor.committeechair

• Edwards, Marc A.

Committee members dc:contributor.committeemember

• Marr, Linsey C.
• Gallagher, Daniel L.

• SRB
• A. polyphaga
• copper
• nutrients

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-05132009-163159

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/32713