{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/32162"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/32162","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Development of New and Alternative Resources for Breeding Low Phytate Soybeans","abstract":"Soybean (Glycine max (L.) Merr) cultivars with genetic reduction in seed phytate concentration will be a valuable resource to livestock producers both nutritionally and economically, as well as in the preservation of the environment. However, due to association with reduced seed germination and field emergence potential, low phytate (LP) soybean cultivars are not being advanced to commercialization. Soybean breeders of LP lines must adjust their techniques and resources in order to efficiently select LP germplasm. Reduced seed germination and field emergence potential associated with LP germplasm eliminates many individuals with the desired LP trait during early generation population development, making improvement of agronomic performance among LP lines difficult. Thus, development of resources for improved phenotyping, genotyping, and early generation population development will facilitate the commercialization of LP cultivars. Discovery of variation for field emergence potential among F2-derived LP families suggests the potential for using a pedigree method for early generation population development in LP germplasm, as it preserves diversity and allows selection for improved field emergence. Integration of markers into a pedigree method will be useful to breeder. An example is BARCSOYSSR_11_1495 with 90-93% selection efficiency in various V99-5089-derived populations. Another resource for improved LP selection is a time-efficient, high-throughput modified iron (Fe) colorimetric phytate assay that does not suffer from inorganic phosphorus (P) concentration interference. Together these tools will provide breeders more accurate selection of LP lines.","abstract_html":"Soybean (Glycine max (L.) Merr) cultivars with genetic reduction in seed phytate concentration will be a valuable resource to livestock producers both nutritionally and economically, as well as in the preservation of the environment. However, due to association with reduced seed germination and field emergence potential, low phytate (LP) soybean cultivars are not being advanced to commercialization. Soybean breeders of LP lines must adjust their techniques and resources in order to efficiently select LP germplasm. Reduced seed germination and field emergence potential associated with LP germplasm eliminates many individuals with the desired LP trait during early generation population development, making improvement of agronomic performance among LP lines difficult. Thus, development of resources for improved phenotyping, genotyping, and early generation population development will facilitate the commercialization of LP cultivars. Discovery of variation for field emergence potential among F2-derived LP families suggests the potential for using a pedigree method for early generation population development in LP germplasm, as it preserves diversity and allows selection for improved field emergence. Integration of markers into a pedigree method will be useful to breeder. An example is BARCSOYSSR_11_1495 with 90-93% selection efficiency in various V99-5089-derived populations. Another resource for improved LP selection is a time-efficient, high-throughput modified iron (Fe) colorimetric phytate assay that does not suffer from inorganic phosphorus (P) concentration interference. Together these tools will provide breeders more accurate selection of LP lines.","abstract_has_math":false,"creators":["Burleson, Sarah Ann"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Crop and Soil Environmental Sciences","degree_department":"Crop and Soil Environmental Sciences","school":null,"contributors":[],"advisors":[],"committee_chairs":["Rainey, Katy Martin"],"committee_members":["Saghai-Maroof, Mohammad A.","Hall, Marla D.","Grabau, Elizabeth A.","Buss, Glenn R."],"year":2011,"date_issued":"2011-04-18","date_published":"2011-04-18","updated_at":"2026-07-22T22:19:04Z","subjects":["Soybean","phytate","inorganic phosphorus","seed germination","field emergence","SSR markers"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-05032011-134607"],"render_values":[{"text":"etd-05032011-134607","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/32162","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Rainey, Katy Martin"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Saghai-Maroof, Mohammad A.","Hall, Marla D.","Grabau, Elizabeth A.","Buss, Glenn R."]},{"key":"dc:contributor.department","label":"Department","values":["Crop and Soil Environmental Sciences"]},{"key":"dc:creator","label":"Author","values":["Burleson, Sarah Ann"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T20:34:56Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T20:34:56Z","2011-05-12"]},{"key":"dc:date.issued","label":"Date","values":["2011-04-18"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop and Soil Environmental Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Soybean","phytate","inorganic phosphorus","seed germination","field emergence","SSR markers"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-05032011-134607"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/32162"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Soybean (Glycine max (L.) Merr) cultivars with genetic reduction in seed phytate concentration will be a valuable resource to livestock producers both nutritionally and economically, as well as in the preservation of the environment. However, due to association with reduced seed germination and field emergence potential, low phytate (LP) soybean cultivars are not being advanced to commercialization. Soybean breeders of LP lines must adjust their techniques and resources in order to efficiently select LP germplasm. Reduced seed germination and field emergence potential associated with LP germplasm eliminates many individuals with the desired LP trait during early generation population development, making improvement of agronomic performance among LP lines difficult. Thus, development of resources for improved phenotyping, genotyping, and early generation population development will facilitate the commercialization of LP cultivars. Discovery of variation for field emergence potential among F2-derived LP families suggests the potential for using a pedigree method for early generation population development in LP germplasm, as it preserves diversity and allows selection for improved field emergence. Integration of markers into a pedigree method will be useful to breeder. An example is BARCSOYSSR_11_1495 with 90-93% selection efficiency in various V99-5089-derived populations. Another resource for improved LP selection is a time-efficient, high-throughput modified iron (Fe) colorimetric phytate assay that does not suffer from inorganic phosphorus (P) concentration interference. Together these tools will provide breeders more accurate selection of LP lines."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:title","label":"Title","values":["Development of New and Alternative Resources for Breeding Low Phytate Soybeans"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Rainey, Katy Martin"],"dc:contributor.committeemember":["Saghai-Maroof, Mohammad A.","Hall, Marla D.","Grabau, Elizabeth A.","Buss, Glenn R."],"dc:contributor.department":["Crop and Soil Environmental Sciences"],"dc:creator":["Burleson, Sarah Ann"],"dc:date.accessioned":["2014-03-14T20:34:56Z"],"dc:date.available":["2014-03-14T20:34:56Z","2011-05-12"],"dc:date.issued":["2011-04-18"],"dc:description.abstract":["Soybean (Glycine max (L.) Merr) cultivars with genetic reduction in seed phytate concentration will be a valuable resource to livestock producers both nutritionally and economically, as well as in the preservation of the environment. However, due to association with reduced seed germination and field emergence potential, low phytate (LP) soybean cultivars are not being advanced to commercialization. Soybean breeders of LP lines must adjust their techniques and resources in order to efficiently select LP germplasm. Reduced seed germination and field emergence potential associated with LP germplasm eliminates many individuals with the desired LP trait during early generation population development, making improvement of agronomic performance among LP lines difficult. Thus, development of resources for improved phenotyping, genotyping, and early generation population development will facilitate the commercialization of LP cultivars. Discovery of variation for field emergence potential among F2-derived LP families suggests the potential for using a pedigree method for early generation population development in LP germplasm, as it preserves diversity and allows selection for improved field emergence. Integration of markers into a pedigree method will be useful to breeder. An example is BARCSOYSSR_11_1495 with 90-93% selection efficiency in various V99-5089-derived populations. Another resource for improved LP selection is a time-efficient, high-throughput modified iron (Fe) colorimetric phytate assay that does not suffer from inorganic phosphorus (P) concentration interference. 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