{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/31915"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/31915","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Synthesis of Nano-Silver Colloids and Their Anti-Microbial Effects","abstract":"The antimicrobial effects of silver nanoparticles were studied. Silver nanoparticles were synthesized through wet chemistry method, and were dispersed in aqueous suspension. With nanoscale silica particles served as heterogeneous nucleation sites, silver nanoparticles were formed anchoring on the silica surface. Suspensions were found to be stable at high silver concentrations as well as over a broad pH range. By varying the processing conditions, diameter of the silver nanoparticles could be controlled between ~2 nm to ~25 nm. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to reveal the formation and the corresponding morphology of the silver-silica coupling nanoparticles. Ultra-violet visible (UV-vis) scanning spectrophotometer was used to detecting the distinct absorption spectrum of silver nanoparticles. The antimicrobial activities of these silver-silica coupling nanoparticles were investigated. E. coli and S aureus were used as representatives of Gram-negative and Gram-positive bacteria, respectively. Bacteriological tests showed either bacterial growth inhibition or cell death occurred, corresponding to different concentrations of silver nanoparticles and the type of bacteria that were testing on. Fluorescent microscopic images were also provided to confirm the bacterial viability after several hours' treatment with silver nanoparticles.","abstract_html":"The antimicrobial effects of silver nanoparticles were studied. Silver nanoparticles were synthesized through wet chemistry method, and were dispersed in aqueous suspension. With nanoscale silica particles served as heterogeneous nucleation sites, silver nanoparticles were formed anchoring on the silica surface. Suspensions were found to be stable at high silver concentrations as well as over a broad pH range. By varying the processing conditions, diameter of the silver nanoparticles could be controlled between ~2 nm to ~25 nm. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to reveal the formation and the corresponding morphology of the silver-silica coupling nanoparticles. Ultra-violet visible (UV-vis) scanning spectrophotometer was used to detecting the distinct absorption spectrum of silver nanoparticles. The antimicrobial activities of these silver-silica coupling nanoparticles were investigated. E. coli and S aureus were used as representatives of Gram-negative and Gram-positive bacteria, respectively. Bacteriological tests showed either bacterial growth inhibition or cell death occurred, corresponding to different concentrations of silver nanoparticles and the type of bacteria that were testing on. Fluorescent microscopic images were also provided to confirm the bacterial viability after several hours' treatment with silver nanoparticles.","abstract_has_math":false,"creators":["Lei, Guangyin"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Materials Science and Engineering","degree_department":"Materials Science and Engineering","school":null,"contributors":[],"advisors":[],"committee_chairs":["Lu, Guo-Quan","Meehan, Kathleen"],"committee_members":["Yang, Zhaomin"],"year":2007,"date_issued":"2007-11-08","date_published":"2007-11-08","updated_at":"2026-07-22T22:20:38Z","subjects":["Bactericide","Escherichia coli","Staphylococcus aureus.","Silver nanoparticles"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-04252008-192512"],"render_values":[{"text":"etd-04252008-192512","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/31915","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Lu, Guo-Quan","Meehan, Kathleen"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Yang, Zhaomin"]},{"key":"dc:contributor.department","label":"Department","values":["Materials Science and Engineering"]},{"key":"dc:creator","label":"Author","values":["Lei, Guangyin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T20:34:17Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T20:34:17Z","2011-01-03"]},{"key":"dc:date.issued","label":"Date","values":["2007-11-08"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Materials Science and Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bactericide","Escherichia coli","Staphylococcus aureus.","Silver nanoparticles"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-04252008-192512"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/31915"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The antimicrobial effects of silver nanoparticles were studied. Silver nanoparticles were synthesized through wet chemistry method, and were dispersed in aqueous suspension. With nanoscale silica particles served as heterogeneous nucleation sites, silver nanoparticles were formed anchoring on the silica surface. Suspensions were found to be stable at high silver concentrations as well as over a broad pH range. By varying the processing conditions, diameter of the silver nanoparticles could be controlled between ~2 nm to ~25 nm. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to reveal the formation and the corresponding morphology of the silver-silica coupling nanoparticles. Ultra-violet visible (UV-vis) scanning spectrophotometer was used to detecting the distinct absorption spectrum of silver nanoparticles. The antimicrobial activities of these silver-silica coupling nanoparticles were investigated. E. coli and S aureus were used as representatives of Gram-negative and Gram-positive bacteria, respectively. Bacteriological tests showed either bacterial growth inhibition or cell death occurred, corresponding to different concentrations of silver nanoparticles and the type of bacteria that were testing on. Fluorescent microscopic images were also provided to confirm the bacterial viability after several hours' treatment with silver nanoparticles."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:title","label":"Title","values":["Synthesis of Nano-Silver Colloids and Their Anti-Microbial Effects"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Lu, Guo-Quan","Meehan, Kathleen"],"dc:contributor.committeemember":["Yang, Zhaomin"],"dc:contributor.department":["Materials Science and Engineering"],"dc:creator":["Lei, Guangyin"],"dc:date.accessioned":["2014-03-14T20:34:17Z"],"dc:date.available":["2014-03-14T20:34:17Z","2011-01-03"],"dc:date.issued":["2007-11-08"],"dc:description.abstract":["The antimicrobial effects of silver nanoparticles were studied. Silver nanoparticles were synthesized through wet chemistry method, and were dispersed in aqueous suspension. With nanoscale silica particles served as heterogeneous nucleation sites, silver nanoparticles were formed anchoring on the silica surface. Suspensions were found to be stable at high silver concentrations as well as over a broad pH range. By varying the processing conditions, diameter of the silver nanoparticles could be controlled between ~2 nm to ~25 nm. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to reveal the formation and the corresponding morphology of the silver-silica coupling nanoparticles. Ultra-violet visible (UV-vis) scanning spectrophotometer was used to detecting the distinct absorption spectrum of silver nanoparticles. The antimicrobial activities of these silver-silica coupling nanoparticles were investigated. E. coli and S aureus were used as representatives of Gram-negative and Gram-positive bacteria, respectively. Bacteriological tests showed either bacterial growth inhibition or cell death occurred, corresponding to different concentrations of silver nanoparticles and the type of bacteria that were testing on. Fluorescent microscopic images were also provided to confirm the bacterial viability after several hours' treatment with silver nanoparticles."],"dc:description.degree":["Master of Science"],"dc:identifier.other":["etd-04252008-192512"],"dc:identifier.uri":["http://hdl.handle.net/10919/31915"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["Bactericide","Escherichia coli","Staphylococcus aureus.","Silver nanoparticles"],"dc:title":["Synthesis of Nano-Silver Colloids and Their Anti-Microbial Effects"],"dc:type":["Thesis"],"thesis:degree_discipline":["Materials Science and Engineering"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:38Z"}