Virginia Tech
Peroxidative protection of parenteral admixture by d-α-tocopherol and its effect on oxidative status of obese cats
Abstract
dc:description.abstractHigh lipid : low dextrose (HL:LD) parenteral admixture (PA) is high in polyunsaturated fatty acids (PUFA) that are sensitive to peroxidation. This study evaluated the antioxidative effect of vitamin E in both HL:LD PA and in obese cats given HL:LD PA. Natural d-α-tocopherol (Vital E-300) was added to HL:LD PA at seven concentrations (8, 12, 16, 24, 32, 48, or 64 IU/g of lipid). PA were exposed to fluorescent light for 24 hours at room temperature. Hydroperoxides were measured at baseline and 24 hours hang time. Significantly lower hydroperoxide concentrations were found with > 24 IU/g of lipid at baseline (P < 0.01). A higher d-α-tocopherol concentration was required (> 48 IU/g lipid) to lower hydroperoxides at 24 hours (P < 0.0001). HL:LD PA with 40 IU/g lipid/day d-α-tocopherol was delivered intravenously to obese cats (PA Toc⁺) over 48 hours. Control cats (PA Toc⁻) received HL:LD PA without a d-α-tocopherol supplementation. Oxidative status of cats was evaluated at baseline and 24, 48, and 96 hours. Cats in both groups exhibited an increase in MDA concentration (time effect; P < 0.0001). WBC-tGSH and WBC-GPx did not change in either group of cats. RBC-tGSH and RBC-GPx changed over time (time effects; P = 0.0005; P = 0.0016, respectively) with the PA Toc⁺ cats exhibiting a higher RBC-tGSH concentration (treatment x time interaction; P = 0.012). Serum α- and γ-tocopherol concentrations increased in PA Toc⁺ cats (treatment effect; P < 0.0001). These findings suggest that d-α-tocopherol significantly alters oxidative status in vivo.
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- masters
- Discipline thesis:degree_discipline
- Biomedical and Veterinary Sciences
- Department dc:contributor.department
- Biomedical and Veterinary Sciences
- Grantor dc:publisher
- Virginia Tech
- Year dc:date.issued
- 2006
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Becvarova, Iveta
- Chair dc:contributor.committeechair
-
- Saker, Korinn E.
- Committee members dc:contributor.committeemember
-
- Troy, Gregory C.
- Swecker, William S. Jr.
Subjects
dc:subject × 5Rights
dc:rights- Statement dc:rights
-
- In Copyright
- Licence dc:rights.uri
- Language dc:language.iso
- en
Identifiers
dc:identifier.*- Dc Identifier Other
- etd-04132006-103946
- OAI identifier oai:identifier
- oai:vtechworks.lib.vt.edu:10919/31707