Virginia Tech
Influence of Seed Treatment on Tobacco Mosaic Virus Incidence in Tobacco Seedlings and Virus Distribution in Greenhouse Transplant Production
Abstract
dc:description.abstractTobacco mosaic virus (TMV) is an economically important pathogen that has been studied for over one hundred years. Seedlings, seed coats, and nutrient solution were assayed for the presence of the virus and seed treatments were tested on seeds. Double antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA) and biological local lesion assay data were collected. Seed coats from seed collected from TMV infected plants were always positive for TMV regardless of chemical treatment. Seed from infected source plants have lower germination than seed from healthy plants. Trisodium phosphate and hydrochloric acid treatments reduced virus infection of seedlings when grown under controlled conditions. Virus particles were serologically and biologically detected in both the leaves and roots of seedlings mechanically inoculated with TMV. Nutrient solution collected from 28 day old seedlings, 12 days post inoculation, tested positive for biologically active TMV by ELISA and infectivity assay. Infected water in float bed production could facilitate viral movement to all seedlings sharing nutrient solution. Seed transmission of TMV was shown to occur at a rate of 0.2%. This is in contrast to other research attempting to demonstrate seed transmission where visual symptoms on seedlings have been used to assess seed transmission.
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- masters
- Discipline thesis:degree_discipline
- Crop and Soil Environmental Sciences
- Department dc:contributor.department
- Crop and Soil Environmental Sciences
- Grantor dc:publisher
- Virginia Tech
- Year dc:date.issued
- 2007
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Loveday, Rachel Ellen Leonard
- Chairs dc:contributor.committeechair
-
- Tolin, Sue A.
- Wilkinson, Carol A.
- Committee member dc:contributor.committeemember
-
- Reed, T. David
Subjects
dc:subject × 3Rights
dc:rights- Statement dc:rights
-
- In Copyright
- Licence dc:rights.uri
Identifiers
dc:identifier.*- Dc Identifier Other
- etd-03022007-150404
- OAI identifier oai:identifier
- oai:vtechworks.lib.vt.edu:10919/31396