Transcriptional profiling of potential regulatory factors modulating defense mechanisms in soybean during Phytophthora sojae infection

Quantitative resistance is controlled by multiple genes and has been shown to be a durable form of resistance to pathogens affecting cultivated crops including soybeans (Glycine max L. Merr). Root rot of soybean caused by Phytophthora sojae ranks among the most damaging soybean diseases. Quantitative resistance has proven durable in soybean against P. sojae, however the molecular mechanisms underlying this form of resistance are still unknown. The objective of this project is to gain insight into molecular basis of quantitative resistance in the soybean-P. sojae pathosystem. The approach was to use global transcriptional profiling based on microarray technology to identify genes that were differentially expressed in four cultivars of soybeans with varying levels of quantitative resistance at different time points during infection by P. sojae. Our results provide a better understanding of the potential regulatory factors that may contribute to quantitative resistance during early hours of P. sojae infection.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Genetics, Bioinformatics, and Computational Biology

Department dc:contributor.department

Genetics, Bioinformatics, and Computational Biology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2010

Author dc:creator

• Waller, LaChelle Monique

Chair dc:contributor.committeechair

• Tyler, Brett M.

Committee members dc:contributor.committeemember

• McDowell, John M.
• Hoeschele, Ina
• Saghai-Maroof, Mohammad A.

• Transcriptional Profiling
• Quantitative Resistance
• Regulatory Factors

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-05042010-145729

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/27541