Synthesis, Characterization, and Antimicrobial Activity of Water-soluble, Tri-carboxylato Amphiphiles

Many previous studies of biological activity in a homologous series of amphiphiles have shown a cut-off effect, where the biological activity increases with an increase in chain length, after which the activity plateaus or weakens. One factor suspected to cause this problem is solubility issues. We have designed several series of very hydrophobic, water-soluble amphiphiles to overcome this problem. Three homologous series containing mobile hydrophobic moieties and two series of epimers containing rigid cholestane moieties have been synthesized; the hydrophobic moiety is connected to the first-generation, Newkome-type dendron via a ureido linker. We have demonstrated that as tris(triethanolammonium) salts, these amphiphiles show excellent solubility in water. The solubilities in aqueous triethanolamine solution of the three series containing mobile hydrophobic moieties are 19,500 to 25,700 μM depending on the formula weight of the homolog, while those containing rigid cholestane moieties are 18,900 and 17,400 μM. Having eliminated the solubility issue, the antimicrobial activity against a broad spectrum of microorganisms has been screened. We have demonstrated that the antimicrobial activity depends on the amphiphile-series, species, chain-length, or epimer specificities, as well as hydrophobicity. The one-tailed, tri-carboxylato amphiphiles are generally better than the other series, with two exceptions. First, the two-tailed tri-carboxylato amphiphiles, <b>3CUr1(11)₂</b> and <b>3CUr1(12)₂</b>, are more active against <i>Cryptococcus neoformans</i>; in fact, both amphiphiles (MICs are 6.9 and 7.2 μM, respectively) are considered to display good antifungal activity. Second, amphiphile <b>3CUr-β-cholestane</b>, whose MIC is 27 μM, is more active against <i>Staphylococcus aureus</i>. Overall, these new tri-carboxylato amphiphiles only exhibit moderate activity with two promising leads. Furthermore, we have demonstrated the intrinsic activity (MIC₀) of the one-tailed, tri-carboxylato amphiphile series (<b>3CUrn</b>) against <i>Mycobacterium smegmatis</i>. All the MIC₀s observed are at least 8-fold lower than the corresponding CMCs. Amphiphile <b>3CUr16</b> is the most active; the MIC₀ is 100-fold smaller than the CMC. With this consideration, we have suggested that the mechanism of action of the antimycobacterial activity in amphiphile <b>3CUr16</b> is not related to detergency.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Chemistry

Department dc:contributor.department

Chemistry

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2007

Author dc:creator

• Sugandhi, Eko Winny

Chair dc:contributor.committeechair

• Gandour, Richard D.

Committee members dc:contributor.committeemember

• Carlier, Paul R.
• Hanson, Brian E.
• Kingston, David G. I.
• Tanko, James M.

• multi-tailed
• multi-headed
• tri-carboxylato
• dendritic
• amphiphile
• solubility
• microorganism
• biological and intrinsic activity

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-02032007-143323

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/26106