{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/140732"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/140732","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"An Investigation of the Interplay Among CX3CR1, Immune Cells, and Gut Microbiota in Lupus-Associated Arthritis and Renal Disease using the MRL/lpr Mouse","abstract":"Systemic lupus erythematosus, or lupus, is a disease where the body's immune system attacks itself, which can lead to tissue injury and organ failure. In this dissertation, different aspects of lupus were studied to better understand the disease. CX3CR1, a surface protein that guides cell migration, was found to influence the production of inflammatory proteins IL-17A and IL-17F, as well as the gut microbiota—bacteria living in the gut. The gut microbiota was observed to influence IL-17A but not IL-17F. When examining lupus-associated kidney disease and joint inflammation arthritis, CX3CR1 was found to affect them differently. The absence of CX3CR1 worsened kidney disease but improved arthritis. Different immune cells such as T cells and myeloid cells were observed to correlate with arthritis and/or kidney disease. A new gut microbiota biomarker for lupus-associated kidney disease, the Lachnospiraceae-to-Oscillospiraceae ratio, was discovered, which may become a new method to help prescreen patients before a kidney biopsy has to be performed. Overall, these findings contribute to scientific knowledge and could lead to targeted therapies to help lupus patients.","abstract_html":"Systemic lupus erythematosus, or lupus, is a disease where the body's immune system attacks itself, which can lead to tissue injury and organ failure. In this dissertation, different aspects of lupus were studied to better understand the disease. CX3CR1, a surface protein that guides cell migration, was found to influence the production of inflammatory proteins IL-17A and IL-17F, as well as the gut microbiota—bacteria living in the gut. The gut microbiota was observed to influence IL-17A but not IL-17F. When examining lupus-associated kidney disease and joint inflammation arthritis, CX3CR1 was found to affect them differently. The absence of CX3CR1 worsened kidney disease but improved arthritis. Different immune cells such as T cells and myeloid cells were observed to correlate with arthritis and/or kidney disease. A new gut microbiota biomarker for lupus-associated kidney disease, the Lachnospiraceae-to-Oscillospiraceae ratio, was discovered, which may become a new method to help prescreen patients before a kidney biopsy has to be performed. Overall, these findings contribute to scientific knowledge and could lead to targeted therapies to help lupus patients.","abstract_has_math":false,"creators":["Estaleen, Rana"],"institution":"Virginia Tech","degree_name":"Doctor of Philosophy","degree_level":"doctoral","degree_discipline":"Biomedical and Veterinary Sciences","degree_department":"Biomedical and Veterinary Sciences","school":null,"contributors":[],"advisors":[],"committee_chairs":["Luo, Xin","Reilly, Christopher Michael"],"committee_members":["Ahmed, S. 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In this dissertation, different aspects of lupus were studied to better understand the disease. CX3CR1, a surface protein that guides cell migration, was found to influence the production of inflammatory proteins IL-17A and IL-17F, as well as the gut microbiota—bacteria living in the gut. The gut microbiota was observed to influence IL-17A but not IL-17F. When examining lupus-associated kidney disease and joint inflammation arthritis, CX3CR1 was found to affect them differently. The absence of CX3CR1 worsened kidney disease but improved arthritis. Different immune cells such as T cells and myeloid cells were observed to correlate with arthritis and/or kidney disease. A new gut microbiota biomarker for lupus-associated kidney disease, the Lachnospiraceae-to-Oscillospiraceae ratio, was discovered, which may become a new method to help prescreen patients before a kidney biopsy has to be performed. Overall, these findings contribute to scientific knowledge and could lead to targeted therapies to help lupus patients."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Doctor of Philosophy"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["ETD"]},{"key":"dc:title","label":"Title","values":["An Investigation of the Interplay Among CX3CR1, Immune Cells, and Gut Microbiota in Lupus-Associated Arthritis and Renal Disease using the MRL/lpr Mouse"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Luo, Xin","Reilly, Christopher Michael"],"dc:contributor.committeemember":["Ahmed, S. 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