{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/118401"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/118401","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"A Role for Inositol Pyrophosphates in Arabidopsis Defense Against Herbivorous Insects","abstract":"Inositol pyrophosphates (PP-InsPs) are a family of molecules recently discovered to be implicated in a number of plant pathways such as auxin regulation, phosphate (Pi) sensing, and jasmonate-(JA)-regulated plant defense. Transgenic plants that overexpress inositol tetrakisphosphate 1-kinase (ITPK1) and the kinase domain of the dual domain diphosphoinositol pentakisphosphate kinase 2 (VIP2KD) have been previously studied to display uniquely elevated PP-InsPs. Here it is reported that the JA defense pathway is constitutively upregulated in VIP2KD OX plants, resulting in a lower rate of herbivory on the transgenic plants. ITPK1 OX, although also having elevated PP-InsPs, was fed upon by insect larvae comparably to Wild-Type Arabidopsis (WT). The data implicate VIP2, InsP8, and possibly the PP-InsP biosynthesis as a whole.","abstract_html":"Inositol pyrophosphates (PP-InsPs) are a family of molecules recently discovered to be implicated in a number of plant pathways such as auxin regulation, phosphate (Pi) sensing, and jasmonate-(JA)-regulated plant defense. Transgenic plants that overexpress inositol tetrakisphosphate 1-kinase (ITPK1) and the kinase domain of the dual domain diphosphoinositol pentakisphosphate kinase 2 (VIP2KD) have been previously studied to display uniquely elevated PP-InsPs. Here it is reported that the JA defense pathway is constitutively upregulated in VIP2KD OX plants, resulting in a lower rate of herbivory on the transgenic plants. ITPK1 OX, although also having elevated PP-InsPs, was fed upon by insect larvae comparably to Wild-Type Arabidopsis (WT). The data implicate VIP2, InsP8, and possibly the PP-InsP biosynthesis as a whole.","abstract_has_math":false,"creators":["Vanwinkle, Ashlynn Brook"],"institution":"Virginia Tech","degree_name":"Master of Science in Life Sciences","degree_level":"masters","degree_discipline":"Biochemistry","degree_department":"Biochemistry","school":null,"contributors":[],"advisors":[],"committee_chairs":["Gillaspy, Glenda E."],"committee_members":["Tholl, Dorothea","Vinauger, Clément"],"year":2024,"date_issued":"2024-03-12","date_published":"2024-03-12","updated_at":"2026-07-22T22:20:25Z","subjects":["Inositol pyrophosphates","Arabidopsis thaliana","insect resistance"],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["vt_gsexam:38047"],"render_values":[{"text":"vt_gsexam:38047","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/10919/118401","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Gillaspy, Glenda E."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Tholl, Dorothea","Vinauger, Clément"]},{"key":"dc:contributor.department","label":"Department","values":["Biochemistry"]},{"key":"dc:creator","label":"Author","values":["Vanwinkle, Ashlynn Brook"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2024-03-13T08:00:12Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2024-03-13T08:00:12Z"]},{"key":"dc:date.issued","label":"Date","values":["2024-03-12"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Life Sciences"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Inositol pyrophosphates","Arabidopsis thaliana","insect resistance"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["vt_gsexam:38047"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10919/118401"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Inositol pyrophosphates (PP-InsPs) are a family of molecules recently discovered to be implicated in a number of plant pathways such as auxin regulation, phosphate (Pi) sensing, and jasmonate-(JA)-regulated plant defense. Transgenic plants that overexpress inositol tetrakisphosphate 1-kinase (ITPK1) and the kinase domain of the dual domain diphosphoinositol pentakisphosphate kinase 2 (VIP2KD) have been previously studied to display uniquely elevated PP-InsPs. Here it is reported that the JA defense pathway is constitutively upregulated in VIP2KD OX plants, resulting in a lower rate of herbivory on the transgenic plants. ITPK1 OX, although also having elevated PP-InsPs, was fed upon by insect larvae comparably to Wild-Type Arabidopsis (WT). The data implicate VIP2, InsP8, and possibly the PP-InsP biosynthesis as a whole."]},{"key":"dc:description.abstractgeneral","label":"General Abstract","values":["Plants and insects have been evolving defenses against each other since they first emerged together post-Cambrian explosion. They each have evolved targeted metabolic pathways to produce chemicals with which to repel, harm, or even trick one another. In Arabidopsis thaliana, one of the most widely studied defense mechanisms is the jasmonic acid defense pathway, which responds to the herbivory of insects like caterpillars by setting off an array of genetic switches. The plant enters a stressed state wherein it represses the genes focused on growth and development and encourages the expression of genes focused on protecting vital resources and thwarting the attacker. This work examines a connection between the phosphate-sensing pathways and the jasmonic acid defense pathways in plants, and the following data show that plants with elevated inositol pyrophosphates (a phosphate storage molecule) are resistant to the herbivory of common pest caterpillars."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science in Life Sciences"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["ETD"]},{"key":"dc:title","label":"Title","values":["A Role for Inositol Pyrophosphates in Arabidopsis Defense Against Herbivorous Insects"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Gillaspy, Glenda E."],"dc:contributor.committeemember":["Tholl, Dorothea","Vinauger, Clément"],"dc:contributor.department":["Biochemistry"],"dc:creator":["Vanwinkle, Ashlynn Brook"],"dc:date.accessioned":["2024-03-13T08:00:12Z"],"dc:date.available":["2024-03-13T08:00:12Z"],"dc:date.issued":["2024-03-12"],"dc:description.abstract":["Inositol pyrophosphates (PP-InsPs) are a family of molecules recently discovered to be implicated in a number of plant pathways such as auxin regulation, phosphate (Pi) sensing, and jasmonate-(JA)-regulated plant defense. Transgenic plants that overexpress inositol tetrakisphosphate 1-kinase (ITPK1) and the kinase domain of the dual domain diphosphoinositol pentakisphosphate kinase 2 (VIP2KD) have been previously studied to display uniquely elevated PP-InsPs. Here it is reported that the JA defense pathway is constitutively upregulated in VIP2KD OX plants, resulting in a lower rate of herbivory on the transgenic plants. ITPK1 OX, although also having elevated PP-InsPs, was fed upon by insect larvae comparably to Wild-Type Arabidopsis (WT). The data implicate VIP2, InsP8, and possibly the PP-InsP biosynthesis as a whole."],"dc:description.abstractgeneral":["Plants and insects have been evolving defenses against each other since they first emerged together post-Cambrian explosion. They each have evolved targeted metabolic pathways to produce chemicals with which to repel, harm, or even trick one another. In Arabidopsis thaliana, one of the most widely studied defense mechanisms is the jasmonic acid defense pathway, which responds to the herbivory of insects like caterpillars by setting off an array of genetic switches. The plant enters a stressed state wherein it represses the genes focused on growth and development and encourages the expression of genes focused on protecting vital resources and thwarting the attacker. This work examines a connection between the phosphate-sensing pathways and the jasmonic acid defense pathways in plants, and the following data show that plants with elevated inositol pyrophosphates (a phosphate storage molecule) are resistant to the herbivory of common pest caterpillars."],"dc:description.degree":["Master of Science in Life Sciences"],"dc:format.medium":["ETD"],"dc:identifier.other":["vt_gsexam:38047"],"dc:identifier.uri":["https://hdl.handle.net/10919/118401"],"dc:language.iso":["en"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["Inositol pyrophosphates","Arabidopsis thaliana","insect resistance"],"dc:title":["A Role for Inositol Pyrophosphates in Arabidopsis Defense Against Herbivorous Insects"],"dc:type":["Thesis"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science in Life Sciences"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:25Z"}