{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/113493"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/113493","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Separation and quantitation of B-6 vitamers in rat plasma by high performance liquid chromatography","abstract":"A high performance liquid chromatographic method was developed for the separation and quantitation of the B-6 vitamers in rat plasma. The separation time was under 20 minutes. The analyses were accomplished using a reverse phase C₁₈ column; a binary system of methanol and heptane sulfonic acid, the ion pair; and a fluorescence detector using 300 nm excitation and 375 nm emission filters. The minimum detectable quantity was determined to be 5 ng for PL and PN and 4 ng for PM. Samples were extracted using potato acid phosphatase; phosphorylated forms of the B-6 vitamers were converted to nonphosphorylated. The B-6 vitamers were measured in plasma from rats whjch had intakes of vitamin B-6 ranging from 7% - 551% of the basal requirement for the vitamin. PL was found to be the predominate B-6 vitamer in plasma from animals consuming <50% B-6 basal requirement and PM for the lowest intake group. PL and total B-6 values were significantly correlated (r = 0.99, p < 0.0001). Significant differences (p < 0.01) in plasma PL and total B-6 concentrations were observed for rats with inadequate and adequate B-6 intakes. The method seemed to be a sensitive indicator of vitamin B-6 status in rats thus indicating potential for use with humans.","abstract_html":"A high performance liquid chromatographic method was developed for the separation and quantitation of the B-6 vitamers in rat plasma. The separation time was under 20 minutes. The analyses were accomplished using a reverse phase C₁₈ column; a binary system of methanol and heptane sulfonic acid, the ion pair; and a fluorescence detector using 300 nm excitation and 375 nm emission filters. The minimum detectable quantity was determined to be 5 ng for PL and PN and 4 ng for PM. Samples were extracted using potato acid phosphatase; phosphorylated forms of the B-6 vitamers were converted to nonphosphorylated. The B-6 vitamers were measured in plasma from rats whjch had intakes of vitamin B-6 ranging from 7% - 551% of the basal requirement for the vitamin. PL was found to be the predominate B-6 vitamer in plasma from animals consuming &lt;50% B-6 basal requirement and PM for the lowest intake group. PL and total B-6 values were significantly correlated (r = 0.99, p &lt; 0.0001). Significant differences (p &lt; 0.01) in plasma PL and total B-6 concentrations were observed for rats with inadequate and adequate B-6 intakes. The method seemed to be a sensitive indicator of vitamin B-6 status in rats thus indicating potential for use with humans.","abstract_has_math":false,"creators":["Hefferan, Theresa E."],"institution":"Virginia Polytechnic Institute and State University","degree_name":"M.S.","degree_level":"masters","degree_discipline":"Human Nutrition and Foods","degree_department":"Human Nutrition and Foods","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1983,"date_issued":"1983","date_published":"1983","updated_at":"2026-07-22T22:19:03Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/113493","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Human Nutrition and Foods"]},{"key":"dc:creator","label":"Author","values":["Hefferan, Theresa E."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2023-01-27T04:47:47Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2023-01-27T04:47:47Z"]},{"key":"dc:date.issued","label":"Date","values":["1983"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Human Nutrition and Foods"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/113493"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A high performance liquid chromatographic method was developed for the separation and quantitation of the B-6 vitamers in rat plasma. The separation time was under 20 minutes. The analyses were accomplished using a reverse phase C₁₈ column; a binary system of methanol and heptane sulfonic acid, the ion pair; and a fluorescence detector using 300 nm excitation and 375 nm emission filters. The minimum detectable quantity was determined to be 5 ng for PL and PN and 4 ng for PM. Samples were extracted using potato acid phosphatase; phosphorylated forms of the B-6 vitamers were converted to nonphosphorylated. The B-6 vitamers were measured in plasma from rats whjch had intakes of vitamin B-6 ranging from 7% - 551% of the basal requirement for the vitamin. PL was found to be the predominate B-6 vitamer in plasma from animals consuming <50% B-6 basal requirement and PM for the lowest intake group. PL and total B-6 values were significantly correlated (r = 0.99, p < 0.0001). Significant differences (p < 0.01) in plasma PL and total B-6 concentrations were observed for rats with inadequate and adequate B-6 intakes. The method seemed to be a sensitive indicator of vitamin B-6 status in rats thus indicating potential for use with humans."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["M.S."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Separation and quantitation of B-6 vitamers in rat plasma by high performance liquid chromatography"]}]}],"canonical_facts":{"dc:contributor.department":["Human Nutrition and Foods"],"dc:creator":["Hefferan, Theresa E."],"dc:date.accessioned":["2023-01-27T04:47:47Z"],"dc:date.available":["2023-01-27T04:47:47Z"],"dc:date.issued":["1983"],"dc:description.abstract":["A high performance liquid chromatographic method was developed for the separation and quantitation of the B-6 vitamers in rat plasma. The separation time was under 20 minutes. The analyses were accomplished using a reverse phase C₁₈ column; a binary system of methanol and heptane sulfonic acid, the ion pair; and a fluorescence detector using 300 nm excitation and 375 nm emission filters. The minimum detectable quantity was determined to be 5 ng for PL and PN and 4 ng for PM. Samples were extracted using potato acid phosphatase; phosphorylated forms of the B-6 vitamers were converted to nonphosphorylated. The B-6 vitamers were measured in plasma from rats whjch had intakes of vitamin B-6 ranging from 7% - 551% of the basal requirement for the vitamin. PL was found to be the predominate B-6 vitamer in plasma from animals consuming <50% B-6 basal requirement and PM for the lowest intake group. PL and total B-6 values were significantly correlated (r = 0.99, p < 0.0001). Significant differences (p < 0.01) in plasma PL and total B-6 concentrations were observed for rats with inadequate and adequate B-6 intakes. The method seemed to be a sensitive indicator of vitamin B-6 status in rats thus indicating potential for use with humans."],"dc:description.degree":["M.S."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/113493"],"dc:language.iso":["en"],"dc:publisher":["Virginia Polytechnic Institute and State University"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Separation and quantitation of B-6 vitamers in rat plasma by high performance liquid chromatography"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Human Nutrition and Foods"],"thesis:degree_level":["masters"],"thesis:degree_name":["M.S."],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:03Z"}