{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/112809"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/112809","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Functional and Morphological Characteristics of Smooth Muscle in the Rat Vagina","abstract":"The vagina is an essential organ of the female reproductive system that has been largely understudied in the field of biomechanics. The ability of the vagina to contract gives rise to a set of active mechanical properties that contribute to the complex function of this organ in-vivo. After briefly reviewing experimental studies on the active properties of the vagina, including the differences in contractility with respect to anatomic regions and orientations, neural pathways, life events, pelvic floor disorders, and surgical mesh treatment, we present our novel experimental studies that aim toward filling existing knowledge gaps on vaginal tissue morphology and contractile function of the vagina. First, we quantified the large heterogeneous deformations that the vagina experiences during contractions for the first time. For this study, vaginal specimens were subjected to isometric planar biaxial tests, during which they were induced to contract via KCl at four applied equi-biaxial stretches. The digital image correlation method was used to perform full-field strain analysis during each contraction. The vagina was found to have anisotropic contractile behavior, generating higher forces and experiencing higher magnitude strains along the longitudinal direction (LD) than along the circumferential direction (CD) during contractions. Then, we performed the first detailed quantification of the distribution and alignment of vaginal smooth muscle and nerves throughout the vagina. Toward this goal, vaginas from adult female rats were subjected to a tissue clearing and immunohistochemistry protocol. Tissue clearing increased the transparency of the specimens such that organs could be imaged without sectioning, thus preserving the 3D architecture of the tissue. This analysis revealed a bimodal distribution of muscle alignment angles, with a significantly higher proportion of muscle oriented along the LD than along the CD of the organ. The morphologic and functional properties of the smooth muscle within the healthy vagina need to be fully investigated so that detrimental alterations in vaginal contractility, such as those caused by pelvic floor disorders and current treatment strategies, can be prevented.","abstract_html":"The vagina is an essential organ of the female reproductive system that has been largely understudied in the field of biomechanics. The ability of the vagina to contract gives rise to a set of active mechanical properties that contribute to the complex function of this organ in-vivo. After briefly reviewing experimental studies on the active properties of the vagina, including the differences in contractility with respect to anatomic regions and orientations, neural pathways, life events, pelvic floor disorders, and surgical mesh treatment, we present our novel experimental studies that aim toward filling existing knowledge gaps on vaginal tissue morphology and contractile function of the vagina. First, we quantified the large heterogeneous deformations that the vagina experiences during contractions for the first time. For this study, vaginal specimens were subjected to isometric planar biaxial tests, during which they were induced to contract via KCl at four applied equi-biaxial stretches. The digital image correlation method was used to perform full-field strain analysis during each contraction. The vagina was found to have anisotropic contractile behavior, generating higher forces and experiencing higher magnitude strains along the longitudinal direction (LD) than along the circumferential direction (CD) during contractions. Then, we performed the first detailed quantification of the distribution and alignment of vaginal smooth muscle and nerves throughout the vagina. Toward this goal, vaginas from adult female rats were subjected to a tissue clearing and immunohistochemistry protocol. Tissue clearing increased the transparency of the specimens such that organs could be imaged without sectioning, thus preserving the 3D architecture of the tissue. This analysis revealed a bimodal distribution of muscle alignment angles, with a significantly higher proportion of muscle oriented along the LD than along the CD of the organ. The morphologic and functional properties of the smooth muscle within the healthy vagina need to be fully investigated so that detrimental alterations in vaginal contractility, such as those caused by pelvic floor disorders and current treatment strategies, can be prevented.","abstract_has_math":false,"creators":["Huntington, Alyssa Joan"],"institution":"Virginia Tech","degree_name":"Doctor of Philosophy","degree_level":"doctoral","degree_discipline":"Engineering Mechanics","degree_department":"Engineering Science and Mechanics","school":null,"contributors":[],"advisors":[],"committee_chairs":["De Vita, Raffaella"],"committee_members":["Case, Scott W.","Boreyko, Jonathan B.","Wayne, Jennifer S.","Wang, Vincent M."],"year":2021,"date_issued":"2021-06-15","date_published":"2021-06-15","updated_at":"2026-07-22T22:20:35Z","subjects":["mechanics","soft tissue","reproductive","smooth muscle"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["vt_gsexam:30113"],"render_values":[{"text":"vt_gsexam:30113","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/112809","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["De Vita, Raffaella"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Case, Scott W.","Boreyko, Jonathan B.","Wayne, Jennifer S.","Wang, Vincent M."]},{"key":"dc:contributor.department","label":"Department","values":["Engineering Science and Mechanics"]},{"key":"dc:creator","label":"Author","values":["Huntington, Alyssa Joan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2022-12-08T07:00:07Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-12-08T07:00:07Z"]},{"key":"dc:date.issued","label":"Date","values":["2021-06-15"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Engineering Mechanics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["mechanics","soft tissue","reproductive","smooth muscle"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["vt_gsexam:30113"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/112809"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The vagina is an essential organ of the female reproductive system that has been largely understudied in the field of biomechanics. The ability of the vagina to contract gives rise to a set of active mechanical properties that contribute to the complex function of this organ in-vivo. After briefly reviewing experimental studies on the active properties of the vagina, including the differences in contractility with respect to anatomic regions and orientations, neural pathways, life events, pelvic floor disorders, and surgical mesh treatment, we present our novel experimental studies that aim toward filling existing knowledge gaps on vaginal tissue morphology and contractile function of the vagina. First, we quantified the large heterogeneous deformations that the vagina experiences during contractions for the first time. For this study, vaginal specimens were subjected to isometric planar biaxial tests, during which they were induced to contract via KCl at four applied equi-biaxial stretches. The digital image correlation method was used to perform full-field strain analysis during each contraction. The vagina was found to have anisotropic contractile behavior, generating higher forces and experiencing higher magnitude strains along the longitudinal direction (LD) than along the circumferential direction (CD) during contractions. Then, we performed the first detailed quantification of the distribution and alignment of vaginal smooth muscle and nerves throughout the vagina. Toward this goal, vaginas from adult female rats were subjected to a tissue clearing and immunohistochemistry protocol. Tissue clearing increased the transparency of the specimens such that organs could be imaged without sectioning, thus preserving the 3D architecture of the tissue. This analysis revealed a bimodal distribution of muscle alignment angles, with a significantly higher proportion of muscle oriented along the LD than along the CD of the organ. The morphologic and functional properties of the smooth muscle within the healthy vagina need to be fully investigated so that detrimental alterations in vaginal contractility, such as those caused by pelvic floor disorders and current treatment strategies, can be prevented."]},{"key":"dc:description.abstractgeneral","label":"General Abstract","values":["The vagina is an essential organ of the female reproductive system that has been largely understudied in the field of biomechanics. The ability of the vagina to contract gives rise to a set of active mechanical properties that contribute to the complex function of this organ in-vivo. After briefly reviewing experimental studies on the active properties of the vagina, including the differences in contractility with respect to anatomic regions and orientations, neural pathways, life events, pelvic floor disorders, and surgical mesh treatment, we present our novel experimental studies that aim toward filling existing knowledge gaps on vaginal tissue morphology and contractile function of the vagina. First, we quantified the large heterogeneous deformations that the vagina experiences during contractions for the first time. For this study, vaginal specimens were subjected to isometric planar biaxial tests, during which they were induced to contract via KCl at four applied equi-biaxial stretches. The digital image correlation method was used to perform full-field strain analysis during each contraction. The vagina was found to have anisotropic contractile behavior, generating higher forces and experiencing higher magnitude strains along the longitudinal direction (LD) than along the circumferential direction (CD) during contractions. Then, we performed the first detailed quantification of the distribution and alignment of vaginal smooth muscle and nerves throughout the vagina. Toward this goal, vaginas from adult female rats were subjected to a tissue clearing and immunohistochemistry protocol. Tissue clearing increased the transparency of the specimens such that organs could be imaged without sectioning, thus preserving the 3D architecture of the tissue. This analysis revealed a bimodal distribution of muscle alignment angles, with a significantly higher proportion of muscle oriented along the LD than along the CD of the organ. The morphologic and functional properties of the smooth muscle within the healthy vagina need to be fully investigated so that detrimental alterations in vaginal contractility, such as those caused by pelvic floor disorders and current treatment strategies, can be prevented."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Doctor of Philosophy"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["ETD"]},{"key":"dc:title","label":"Title","values":["Functional and Morphological Characteristics of Smooth Muscle in the Rat Vagina"]}]}],"canonical_facts":{"dc:contributor.committeechair":["De Vita, Raffaella"],"dc:contributor.committeemember":["Case, Scott W.","Boreyko, Jonathan B.","Wayne, Jennifer S.","Wang, Vincent M."],"dc:contributor.department":["Engineering Science and Mechanics"],"dc:creator":["Huntington, Alyssa Joan"],"dc:date.accessioned":["2022-12-08T07:00:07Z"],"dc:date.available":["2022-12-08T07:00:07Z"],"dc:date.issued":["2021-06-15"],"dc:description.abstract":["The vagina is an essential organ of the female reproductive system that has been largely understudied in the field of biomechanics. The ability of the vagina to contract gives rise to a set of active mechanical properties that contribute to the complex function of this organ in-vivo. After briefly reviewing experimental studies on the active properties of the vagina, including the differences in contractility with respect to anatomic regions and orientations, neural pathways, life events, pelvic floor disorders, and surgical mesh treatment, we present our novel experimental studies that aim toward filling existing knowledge gaps on vaginal tissue morphology and contractile function of the vagina. First, we quantified the large heterogeneous deformations that the vagina experiences during contractions for the first time. For this study, vaginal specimens were subjected to isometric planar biaxial tests, during which they were induced to contract via KCl at four applied equi-biaxial stretches. The digital image correlation method was used to perform full-field strain analysis during each contraction. The vagina was found to have anisotropic contractile behavior, generating higher forces and experiencing higher magnitude strains along the longitudinal direction (LD) than along the circumferential direction (CD) during contractions. Then, we performed the first detailed quantification of the distribution and alignment of vaginal smooth muscle and nerves throughout the vagina. Toward this goal, vaginas from adult female rats were subjected to a tissue clearing and immunohistochemistry protocol. Tissue clearing increased the transparency of the specimens such that organs could be imaged without sectioning, thus preserving the 3D architecture of the tissue. This analysis revealed a bimodal distribution of muscle alignment angles, with a significantly higher proportion of muscle oriented along the LD than along the CD of the organ. The morphologic and functional properties of the smooth muscle within the healthy vagina need to be fully investigated so that detrimental alterations in vaginal contractility, such as those caused by pelvic floor disorders and current treatment strategies, can be prevented."],"dc:description.abstractgeneral":["The vagina is an essential organ of the female reproductive system that has been largely understudied in the field of biomechanics. The ability of the vagina to contract gives rise to a set of active mechanical properties that contribute to the complex function of this organ in-vivo. After briefly reviewing experimental studies on the active properties of the vagina, including the differences in contractility with respect to anatomic regions and orientations, neural pathways, life events, pelvic floor disorders, and surgical mesh treatment, we present our novel experimental studies that aim toward filling existing knowledge gaps on vaginal tissue morphology and contractile function of the vagina. First, we quantified the large heterogeneous deformations that the vagina experiences during contractions for the first time. For this study, vaginal specimens were subjected to isometric planar biaxial tests, during which they were induced to contract via KCl at four applied equi-biaxial stretches. The digital image correlation method was used to perform full-field strain analysis during each contraction. The vagina was found to have anisotropic contractile behavior, generating higher forces and experiencing higher magnitude strains along the longitudinal direction (LD) than along the circumferential direction (CD) during contractions. Then, we performed the first detailed quantification of the distribution and alignment of vaginal smooth muscle and nerves throughout the vagina. Toward this goal, vaginas from adult female rats were subjected to a tissue clearing and immunohistochemistry protocol. Tissue clearing increased the transparency of the specimens such that organs could be imaged without sectioning, thus preserving the 3D architecture of the tissue. This analysis revealed a bimodal distribution of muscle alignment angles, with a significantly higher proportion of muscle oriented along the LD than along the CD of the organ. The morphologic and functional properties of the smooth muscle within the healthy vagina need to be fully investigated so that detrimental alterations in vaginal contractility, such as those caused by pelvic floor disorders and current treatment strategies, can be prevented."],"dc:description.degree":["Doctor of Philosophy"],"dc:format.medium":["ETD"],"dc:identifier.other":["vt_gsexam:30113"],"dc:identifier.uri":["http://hdl.handle.net/10919/112809"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["mechanics","soft tissue","reproductive","smooth muscle"],"dc:title":["Functional and Morphological Characteristics of Smooth Muscle in the Rat Vagina"],"dc:type":["Dissertation"],"thesis:degree_discipline":["Engineering Mechanics"],"thesis:degree_level":["doctoral"],"thesis:degree_name":["Doctor of Philosophy"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:35Z"}