Macrophage regulation of T lymphocyte subsets in normal and tumor- bearing hosts

The mixed lymphocyte reaction (MLR) was used to examine the blastogenic response of normal and tumor-bearing host (TBH) murine T lymphocyte subpopulations in the presence of normal and TBH macrophage (MΦ)-derived factors. Rapidly proliferating normal and TBH Lyt-1⁺ T cells were inhibited by high doses of normal or TBH MΦ culture supernatants. This inhibition could be removed by dilution of the MΦ supernatants. TBH MΦ culture supernatants exhibited high dose inhibition and low dose enhancement of slowly proliferating normal and TBH Lyt-2⁺ T cells. Normal host MΦ culture supernatants inhibited normal host Lyt-2⁺ cells at high doses and enhanced at low doses, but enhanced TBH Lyt-2⁺ cell proliferation at both low and high doses. Enhancement of the proliferative response of Lyt-2⁺ cytotoxic/suppressor cells in the presence of normal and TBH MΦ culture supernatants is discussed as to its application to the progressive decline of cell-mediated immunity in the TBH. MΦ culture supernatants were found to contain two high molecular weight (80,000 and 45,000) inhibitory factors, and a lower molecular weight (13,000) enhancing factor as evidenced by SDS-polyacrylamide gel electrophoresis and G-1OO Sephadex column chromatography. The data suggest that the enhancer may be interleukin 1. Prostaglandins and interferon are potent inhibitors of the immune response, and although they were detected in very small quantities in the MΦ culture supernatants, their contribution to the inhibitory activity observed in those supernatants could not be ruled out.

Name thesis:degree_name

M.S.

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Microbiology

Department dc:contributor.department

Microbiology

Grantor dc:publisher

Virginia Polytechnic Institute and State University

Year dc:date.issued

1983

Author dc:creator

• Black, Sandra Pettit

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/10919/112478

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/112478