{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/109899"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/109899","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Flight activity and hemolymph diacylglyceride concentrations in Heliothis zea (Boddie) (Lepidoptera:Noctuidae)","abstract":"In this study, a sensitive high performance thin-layer chromatography method (HPTLC) was developed to detect lipids in microgram (ug) quantities of hemolymph. The effect of age, diurnal rhythms, and flight activity on hemolymph 1,2-diacylglyceride concentrations in male and female Heliothis zea (Boddie) (Lepidoptera: Noctuidae) were quantitated. The HPTLC method involved a two-stage, one dimensional solvent system, 1 ). benzene:ether(diethyl):ethanol:acetic acid, 50:40:2.0:0.2; 2). hexane:heptane: ether (diethyl): acetic acid, 63:18:18:1. The solvent system separated alkanes, steryl ester, mono-, di- and triacylglycerides, free fatty acids and cholesterol. Lipids were detected with a fluorescent reagent, 1 mM 6-p-toludino-2-napthalenesulfonic acid (TNS) in 95% methanol. Detection sensitivities were 50 ng qualitatively and 500 ng quantitatively. Most male and female Heliothis zea flight activity occurred between 17⁰⁰ and 3⁰⁰ EST each day. Male flight activity after the day of eclosion through 6 days was 0.8, 1.0, 7.0, 3.3, 3.8, 2.8 and 4.0 times the flight activity of the females. Females had a unimodal pattern of flight activity between 17⁰⁰ and 19⁰⁰ Males had a bimodal pattern between 17⁰⁰ and 19⁰⁰, but another higher peak of flight activity existed between 20⁰⁰ and 24⁰⁰. The hemolymph of Heliothis zea males and females prior to flight (17⁰⁰) was composed of mainly diacylglycerides (50% w/w), and triacylglycerides (35% w/w). In addition, cholesterol esters (2%) and less than 1% monoacylglycerides and cholesterol were present, but free fatty acids were not detectable ( <0.5 ug/ul). The lipid class, 1,2-diacylglycerides, apparently are used for flight energy. They occured in the highest concentrations in both males and females prior to flight (17⁰⁰) (approximately 32 ug/ul), then decreased steadily throughout flight to approximately 16 ug/ul at 2⁰⁰ when flight activity was reduced or ceased. Males and females appear to have similar 1,2-diacylglyceride concentrations prior to, during and after flight activity.","abstract_html":"In this study, a sensitive high performance thin-layer chromatography method (HPTLC) was developed to detect lipids in microgram (ug) quantities of hemolymph. The effect of age, diurnal rhythms, and flight activity on hemolymph 1,2-diacylglyceride concentrations in male and female Heliothis zea (Boddie) (Lepidoptera: Noctuidae) were quantitated. The HPTLC method involved a two-stage, one dimensional solvent system, 1 ). benzene:ether(diethyl):ethanol:acetic acid, 50:40:2.0:0.2; 2). hexane:heptane: ether (diethyl): acetic acid, 63:18:18:1. The solvent system separated alkanes, steryl ester, mono-, di- and triacylglycerides, free fatty acids and cholesterol. Lipids were detected with a fluorescent reagent, 1 mM 6-p-toludino-2-napthalenesulfonic acid (TNS) in 95% methanol. Detection sensitivities were 50 ng qualitatively and 500 ng quantitatively. Most male and female Heliothis zea flight activity occurred between 17⁰⁰ and 3⁰⁰ EST each day. Male flight activity after the day of eclosion through 6 days was 0.8, 1.0, 7.0, 3.3, 3.8, 2.8 and 4.0 times the flight activity of the females. Females had a unimodal pattern of flight activity between 17⁰⁰ and 19⁰⁰ Males had a bimodal pattern between 17⁰⁰ and 19⁰⁰, but another higher peak of flight activity existed between 20⁰⁰ and 24⁰⁰. The hemolymph of Heliothis zea males and females prior to flight (17⁰⁰) was composed of mainly diacylglycerides (50% w/w), and triacylglycerides (35% w/w). In addition, cholesterol esters (2%) and less than 1% monoacylglycerides and cholesterol were present, but free fatty acids were not detectable ( &lt;0.5 ug/ul). The lipid class, 1,2-diacylglycerides, apparently are used for flight energy. They occured in the highest concentrations in both males and females prior to flight (17⁰⁰) (approximately 32 ug/ul), then decreased steadily throughout flight to approximately 16 ug/ul at 2⁰⁰ when flight activity was reduced or ceased. Males and females appear to have similar 1,2-diacylglyceride concentrations prior to, during and after flight activity.","abstract_has_math":false,"creators":["Judge, David Nixon"],"institution":"Virginia Polytechnic Institute and State University","degree_name":"M.S.","degree_level":"masters","degree_discipline":"Entomology","degree_department":"Entomology","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1988,"date_issued":"1988","date_published":"1988","updated_at":"2026-07-22T22:20:16Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/109899","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Entomology"]},{"key":"dc:creator","label":"Author","values":["Judge, David Nixon"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2022-05-09T15:45:00Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-05-09T15:45:00Z"]},{"key":"dc:date.issued","label":"Date","values":["1988"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Entomology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/109899"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In this study, a sensitive high performance thin-layer chromatography method (HPTLC) was developed to detect lipids in microgram (ug) quantities of hemolymph. The effect of age, diurnal rhythms, and flight activity on hemolymph 1,2-diacylglyceride concentrations in male and female Heliothis zea (Boddie) (Lepidoptera: Noctuidae) were quantitated. The HPTLC method involved a two-stage, one dimensional solvent system, 1 ). benzene:ether(diethyl):ethanol:acetic acid, 50:40:2.0:0.2; 2). hexane:heptane: ether (diethyl): acetic acid, 63:18:18:1. The solvent system separated alkanes, steryl ester, mono-, di- and triacylglycerides, free fatty acids and cholesterol. Lipids were detected with a fluorescent reagent, 1 mM 6-p-toludino-2-napthalenesulfonic acid (TNS) in 95% methanol. Detection sensitivities were 50 ng qualitatively and 500 ng quantitatively. Most male and female Heliothis zea flight activity occurred between 17⁰⁰ and 3⁰⁰ EST each day. Male flight activity after the day of eclosion through 6 days was 0.8, 1.0, 7.0, 3.3, 3.8, 2.8 and 4.0 times the flight activity of the females. Females had a unimodal pattern of flight activity between 17⁰⁰ and 19⁰⁰ Males had a bimodal pattern between 17⁰⁰ and 19⁰⁰, but another higher peak of flight activity existed between 20⁰⁰ and 24⁰⁰. The hemolymph of Heliothis zea males and females prior to flight (17⁰⁰) was composed of mainly diacylglycerides (50% w/w), and triacylglycerides (35% w/w). In addition, cholesterol esters (2%) and less than 1% monoacylglycerides and cholesterol were present, but free fatty acids were not detectable ( <0.5 ug/ul). The lipid class, 1,2-diacylglycerides, apparently are used for flight energy. They occured in the highest concentrations in both males and females prior to flight (17⁰⁰) (approximately 32 ug/ul), then decreased steadily throughout flight to approximately 16 ug/ul at 2⁰⁰ when flight activity was reduced or ceased. Males and females appear to have similar 1,2-diacylglyceride concentrations prior to, during and after flight activity."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["M.S."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Flight activity and hemolymph diacylglyceride concentrations in Heliothis zea (Boddie) (Lepidoptera:Noctuidae)"]}]}],"canonical_facts":{"dc:contributor.department":["Entomology"],"dc:creator":["Judge, David Nixon"],"dc:date.accessioned":["2022-05-09T15:45:00Z"],"dc:date.available":["2022-05-09T15:45:00Z"],"dc:date.issued":["1988"],"dc:description.abstract":["In this study, a sensitive high performance thin-layer chromatography method (HPTLC) was developed to detect lipids in microgram (ug) quantities of hemolymph. The effect of age, diurnal rhythms, and flight activity on hemolymph 1,2-diacylglyceride concentrations in male and female Heliothis zea (Boddie) (Lepidoptera: Noctuidae) were quantitated. The HPTLC method involved a two-stage, one dimensional solvent system, 1 ). benzene:ether(diethyl):ethanol:acetic acid, 50:40:2.0:0.2; 2). hexane:heptane: ether (diethyl): acetic acid, 63:18:18:1. The solvent system separated alkanes, steryl ester, mono-, di- and triacylglycerides, free fatty acids and cholesterol. Lipids were detected with a fluorescent reagent, 1 mM 6-p-toludino-2-napthalenesulfonic acid (TNS) in 95% methanol. Detection sensitivities were 50 ng qualitatively and 500 ng quantitatively. Most male and female Heliothis zea flight activity occurred between 17⁰⁰ and 3⁰⁰ EST each day. Male flight activity after the day of eclosion through 6 days was 0.8, 1.0, 7.0, 3.3, 3.8, 2.8 and 4.0 times the flight activity of the females. Females had a unimodal pattern of flight activity between 17⁰⁰ and 19⁰⁰ Males had a bimodal pattern between 17⁰⁰ and 19⁰⁰, but another higher peak of flight activity existed between 20⁰⁰ and 24⁰⁰. The hemolymph of Heliothis zea males and females prior to flight (17⁰⁰) was composed of mainly diacylglycerides (50% w/w), and triacylglycerides (35% w/w). In addition, cholesterol esters (2%) and less than 1% monoacylglycerides and cholesterol were present, but free fatty acids were not detectable ( <0.5 ug/ul). The lipid class, 1,2-diacylglycerides, apparently are used for flight energy. They occured in the highest concentrations in both males and females prior to flight (17⁰⁰) (approximately 32 ug/ul), then decreased steadily throughout flight to approximately 16 ug/ul at 2⁰⁰ when flight activity was reduced or ceased. Males and females appear to have similar 1,2-diacylglyceride concentrations prior to, during and after flight activity."],"dc:description.degree":["M.S."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/109899"],"dc:language.iso":["en"],"dc:publisher":["Virginia Polytechnic Institute and State University"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Flight activity and hemolymph diacylglyceride concentrations in Heliothis zea (Boddie) (Lepidoptera:Noctuidae)"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Entomology"],"thesis:degree_level":["masters"],"thesis:degree_name":["M.S."],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:16Z"}