{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/103878"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/103878","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Evaluating the role of the fission yeast cyclin B Cdc13 in cell size homeostasis","abstract":"Most cellular proteins retain a stable concentration as cells grow and divide, but there are exceptions. Some cell cycle regulators change in concentration with cell size. In fission yeast, Cdc13 (cyclin B), an important activator of the core cell cycle kinase Cdc2 (CDK1), increases in concentration as cells grow. It has been proposed that the concentration of such cell cycle regulators serves as a proxy for cell size and makes cell cycle progression dependent on cell size, thereby contributing to cell size homeostasis. The underlying mechanisms for the size-dependent scaling of these cell cycle regulators are poorly understood. Here, I show that Cdc13 protein concentration, but not mRNA concentration, increases with cell size. Furthermore, only the nuclear, but not the cytoplasmic, fraction of Cdc13 increases in concentration as cell size increases. Computational modeling along with half-life measurements suggests that stabilization of Cdc13 in the nucleus plays an important role in establishing this pattern. Taken together, my results suggest that Cdc13 scales with time, and therefore only indirectly—not directly—with cell size. This leaves open the possibility that Cdc13 contributes to cell size homeostasis, but in a different way than originally proposed.","abstract_html":"Most cellular proteins retain a stable concentration as cells grow and divide, but there are exceptions. Some cell cycle regulators change in concentration with cell size. In fission yeast, Cdc13 (cyclin B), an important activator of the core cell cycle kinase Cdc2 (CDK1), increases in concentration as cells grow. It has been proposed that the concentration of such cell cycle regulators serves as a proxy for cell size and makes cell cycle progression dependent on cell size, thereby contributing to cell size homeostasis. The underlying mechanisms for the size-dependent scaling of these cell cycle regulators are poorly understood. Here, I show that Cdc13 protein concentration, but not mRNA concentration, increases with cell size. Furthermore, only the nuclear, but not the cytoplasmic, fraction of Cdc13 increases in concentration as cell size increases. Computational modeling along with half-life measurements suggests that stabilization of Cdc13 in the nucleus plays an important role in establishing this pattern. Taken together, my results suggest that Cdc13 scales with time, and therefore only indirectly—not directly—with cell size. This leaves open the possibility that Cdc13 contributes to cell size homeostasis, but in a different way than originally proposed.","abstract_has_math":false,"creators":["Rogers, Jessie Michaela"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Biological Sciences","degree_department":"Biological Sciences","school":null,"contributors":[],"advisors":[],"committee_chairs":["Hauf, Silke"],"committee_members":["Price, Michael Scott","Winkel, Brenda S. 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Some cell cycle regulators change in concentration with cell size. In fission yeast, Cdc13 (cyclin B), an important activator of the core cell cycle kinase Cdc2 (CDK1), increases in concentration as cells grow. It has been proposed that the concentration of such cell cycle regulators serves as a proxy for cell size and makes cell cycle progression dependent on cell size, thereby contributing to cell size homeostasis. The underlying mechanisms for the size-dependent scaling of these cell cycle regulators are poorly understood. Here, I show that Cdc13 protein concentration, but not mRNA concentration, increases with cell size. Furthermore, only the nuclear, but not the cytoplasmic, fraction of Cdc13 increases in concentration as cell size increases. Computational modeling along with half-life measurements suggests that stabilization of Cdc13 in the nucleus plays an important role in establishing this pattern. Taken together, my results suggest that Cdc13 scales with time, and therefore only indirectly—not directly—with cell size. This leaves open the possibility that Cdc13 contributes to cell size homeostasis, but in a different way than originally proposed."]},{"key":"dc:description.abstractgeneral","label":"General Abstract","values":["Cells maintain their size very efficiently, but how they manage to do so is not well characterized. It has been suggested that cells sense their size by the size-dependent concentration changes of cell cycle proteins. I have investigated how cyclin B may serve as such a proxy for cell size in fission yeast. My data suggest that fission yeast cyclin B indirectly scales with cell size through an unknown time-based mechanism."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["ETD"]},{"key":"dc:title","label":"Title","values":["Evaluating the role of the fission yeast cyclin B Cdc13 in cell size homeostasis"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Hauf, Silke"],"dc:contributor.committeemember":["Price, Michael Scott","Winkel, Brenda S. J.","Chen, Jing"],"dc:contributor.department":["Biological Sciences"],"dc:creator":["Rogers, Jessie Michaela"],"dc:date.accessioned":["2021-06-16T08:00:43Z"],"dc:date.available":["2021-06-16T08:00:43Z"],"dc:date.issued":["2021-06-15"],"dc:description.abstract":["Most cellular proteins retain a stable concentration as cells grow and divide, but there are exceptions. Some cell cycle regulators change in concentration with cell size. 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