{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/101172"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/101172","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Degradation of tertiary butyl alcohol by a Pseudomonas sp. isolated from groundwater","abstract":"A <i>Pseudomonas</i> sp. capable of degrading tertiary butyl alcohol (TBA) as a sole carbon source, was isolated from a groundwater aquifer (50 ft. deep) at a petroleum refinery. The most probable number (MPN) of TBA degrading microorganisms was 4.9 x 10³ organisms/g (dry wt) of subsurface soil. Pristine subsurface soils, which did not have a history of petroleum contamination, had MPNs of < 2 TBA degrading organisms/g (dry wt) indicating a natural enrichment process at the refinery site. The Q<sub>O2</sub> of <i>Pseudomonas</i> sp. was 4.2 ml O₂/mg dry wt/h when TBA was the substrate. The optimum pH for growth was 7.0. The organism grew faster in continuous culture when TBA was the sole carbon source with a doubling time 33.6 h. The doubling time in batch culture was 112.3 h. When yeast extract was added to a mineral salts + TBA medium to concentrations greater than 1 mg/ml, TBA degradation was inhibited. When the yeast extract concentration was 0.1 mg/ml, a diauxy effect was seen in the growth rate. This suggested that TBA degradation by <i>Pseudomonas</i> sp. was subject to a regulatory mechanism.","abstract_html":"A &lt;i&gt;Pseudomonas&lt;/i&gt; sp. capable of degrading tertiary butyl alcohol (TBA) as a sole carbon source, was isolated from a groundwater aquifer (50 ft. deep) at a petroleum refinery. The most probable number (MPN) of TBA degrading microorganisms was 4.9 x 10³ organisms/g (dry wt) of subsurface soil. Pristine subsurface soils, which did not have a history of petroleum contamination, had MPNs of &lt; 2 TBA degrading organisms/g (dry wt) indicating a natural enrichment process at the refinery site. The Q&lt;sub&gt;O2&lt;/sub&gt; of &lt;i&gt;Pseudomonas&lt;/i&gt; sp. was 4.2 ml O₂/mg dry wt/h when TBA was the substrate. The optimum pH for growth was 7.0. The organism grew faster in continuous culture when TBA was the sole carbon source with a doubling time 33.6 h. The doubling time in batch culture was 112.3 h. When yeast extract was added to a mineral salts + TBA medium to concentrations greater than 1 mg/ml, TBA degradation was inhibited. When the yeast extract concentration was 0.1 mg/ml, a diauxy effect was seen in the growth rate. This suggested that TBA degradation by &lt;i&gt;Pseudomonas&lt;/i&gt; sp. was subject to a regulatory mechanism.","abstract_has_math":false,"creators":["Chadduck, James B."],"institution":"Virginia Polytechnic Institute and State University","degree_name":"M.S.","degree_level":"masters","degree_discipline":"Microbiology","degree_department":"Microbiology","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1987,"date_issued":"1987","date_published":"1987","updated_at":"2026-07-22T22:19:21Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/101172","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Microbiology"]},{"key":"dc:creator","label":"Author","values":["Chadduck, James B."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2020-12-14T16:34:42Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2020-12-14T16:34:42Z"]},{"key":"dc:date.issued","label":"Date","values":["1987"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/101172"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A <i>Pseudomonas</i> sp. capable of degrading tertiary butyl alcohol (TBA) as a sole carbon source, was isolated from a groundwater aquifer (50 ft. deep) at a petroleum refinery. The most probable number (MPN) of TBA degrading microorganisms was 4.9 x 10³ organisms/g (dry wt) of subsurface soil. Pristine subsurface soils, which did not have a history of petroleum contamination, had MPNs of < 2 TBA degrading organisms/g (dry wt) indicating a natural enrichment process at the refinery site. The Q<sub>O2</sub> of <i>Pseudomonas</i> sp. was 4.2 ml O₂/mg dry wt/h when TBA was the substrate. The optimum pH for growth was 7.0. The organism grew faster in continuous culture when TBA was the sole carbon source with a doubling time 33.6 h. The doubling time in batch culture was 112.3 h. When yeast extract was added to a mineral salts + TBA medium to concentrations greater than 1 mg/ml, TBA degradation was inhibited. When the yeast extract concentration was 0.1 mg/ml, a diauxy effect was seen in the growth rate. This suggested that TBA degradation by <i>Pseudomonas</i> sp. was subject to a regulatory mechanism."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["M.S."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Degradation of tertiary butyl alcohol by a Pseudomonas sp. isolated from groundwater"]}]}],"canonical_facts":{"dc:contributor.department":["Microbiology"],"dc:creator":["Chadduck, James B."],"dc:date.accessioned":["2020-12-14T16:34:42Z"],"dc:date.available":["2020-12-14T16:34:42Z"],"dc:date.issued":["1987"],"dc:description.abstract":["A <i>Pseudomonas</i> sp. capable of degrading tertiary butyl alcohol (TBA) as a sole carbon source, was isolated from a groundwater aquifer (50 ft. deep) at a petroleum refinery. The most probable number (MPN) of TBA degrading microorganisms was 4.9 x 10³ organisms/g (dry wt) of subsurface soil. Pristine subsurface soils, which did not have a history of petroleum contamination, had MPNs of < 2 TBA degrading organisms/g (dry wt) indicating a natural enrichment process at the refinery site. The Q<sub>O2</sub> of <i>Pseudomonas</i> sp. was 4.2 ml O₂/mg dry wt/h when TBA was the substrate. The optimum pH for growth was 7.0. The organism grew faster in continuous culture when TBA was the sole carbon source with a doubling time 33.6 h. The doubling time in batch culture was 112.3 h. When yeast extract was added to a mineral salts + TBA medium to concentrations greater than 1 mg/ml, TBA degradation was inhibited. When the yeast extract concentration was 0.1 mg/ml, a diauxy effect was seen in the growth rate. This suggested that TBA degradation by <i>Pseudomonas</i> sp. was subject to a regulatory mechanism."],"dc:description.degree":["M.S."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/101172"],"dc:language.iso":["en"],"dc:publisher":["Virginia Polytechnic Institute and State University"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Degradation of tertiary butyl alcohol by a Pseudomonas sp. isolated from groundwater"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["masters"],"thesis:degree_name":["M.S."],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:21Z"}