Virginia Tech
A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model
Abstract
dc:description.abstractThis thesis project serves to fill experimental gaps needed to advance the goal of performing pre-clinical trials using an orthotopic rat glioblastoma model to evaluate the efficacy of high-frequency electroporation (H-FIRE) and QUAD-CTX tumor receptor-targeted cytotoxic conjugate therapies, individually and in combination, in selectively and thoroughly treating glioblastoma multiforme. In order to achieve this, an appropriate model must be developed and characterized. I have transduced F98 rat glioma cells to express red-shifted firefly luciferase, which will facilitate longitudinal tumor monitoring in vivo through bioluminescent imaging. I have characterized their response to H-FIRE relative to DI TNC1 rat astrocytes. I have demonstrated the presence of the molecular targets of QUAD in F98 cells. The in vitro characterization of this model has enabled preclinical studies of this promising glioblastoma therapy in an immunocompetent rat model, an important step before advancing ultimately to clinical human trials.
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- masters
- Discipline thesis:degree_discipline
- Biomedical Engineering
- Department dc:contributor.department
- Department of Biomedical Engineering and Mechanics
- Grantor dc:publisher
- Virginia Tech
- Year dc:date.issued
- 2020
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Thomas, Sean C.
- Chair dc:contributor.committeechair
-
- Verbridge, Scott
- Committee members dc:contributor.committeemember
-
- Robertson, John L.
- Davalos, Rafael V.
Subjects
dc:subject × 11Rights
dc:rights- Statement dc:rights
-
- In Copyright
- Licence dc:rights.uri
Identifiers
dc:identifier.*- Dc Identifier Other
- vt_gsexam:27779
- OAI identifier oai:identifier
- oai:vtechworks.lib.vt.edu:10919/100772