{"id":{"repo_id":"vilnius","oai_identifier":"oai:vu.lt:elaba:76894409"},"canonical_url":"https://search.dev.ndltd.org/etd/vilnius/oai:vu.lt:elaba:76894409","repository":{"repo_id":"vilnius","name":"Vilnius University","base_url":"https://epublications.vu.lt/oai"},"display":{"title":"Su onkologinėmis ligomis siejamas pre-iRNR splaisingas: splaisingo veiksnių ir hipoksinės mikroaplinkos tyrimas /","abstract":"Oncological diseases remain one of the unsolved problems of the entire society as the oncology-related morbidity and mortality has been systematically increasing. As pre-mRNA splicing is a highly important process in regulating gene expression, it is not surprising that changes in splicing have been observed in many different diseases. Unfortunately, the reasons for the expression of disease-associated mRNA isoforms and their regulatory factors have not been completely understood yet. This study describes the research of splicing factors and the expression of tumor-associated mRNA isoforms in healthy and tumor gastrointestinal organ tissues and tumors cell lines, and examines whether/how hypoxic microenvironment in tumor cell lines affects tumor-associated pre-mRNA splicing changes. Studies in this work have shown differences in the expression of SRSF1, SRSF2, U2AF65, U2AF35 and KHSRP splicing factors and tumor-associated Fas and Rac1 mRNA isoforms in tumor tissues and commonly used tumor cell lines. Our results indicate that reduced oxygen content in the cell environment (hypoxia) affects Fas pre-mRNA alternative splicing. The results obtained in this work provide valuable information about the processes initiating diseases in various systems.","abstract_html":"Oncological diseases remain one of the unsolved problems of the entire society as the oncology-related morbidity and mortality has been systematically increasing. As pre-mRNA splicing is a highly important process in regulating gene expression, it is not surprising that changes in splicing have been observed in many different diseases. Unfortunately, the reasons for the expression of disease-associated mRNA isoforms and their regulatory factors have not been completely understood yet. This study describes the research of splicing factors and the expression of tumor-associated mRNA isoforms in healthy and tumor gastrointestinal organ tissues and tumors cell lines, and examines whether/how hypoxic microenvironment in tumor cell lines affects tumor-associated pre-mRNA splicing changes. Studies in this work have shown differences in the expression of SRSF1, SRSF2, U2AF65, U2AF35 and KHSRP splicing factors and tumor-associated Fas and Rac1 mRNA isoforms in tumor tissues and commonly used tumor cell lines. Our results indicate that reduced oxygen content in the cell environment (hypoxia) affects Fas pre-mRNA alternative splicing. The results obtained in this work provide valuable information about the processes initiating diseases in various systems.","abstract_has_math":false,"creators":["Pečiulienė, Inga,"],"institution":"Institutional Repository of Vilnius University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020","date_published":"2020","updated_at":"2026-07-24T05:55:48Z","subjects":["dissertation ; hypoxia ; cancer ; pre-mRNA ; splicing"],"languages":["lit"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://repository.vu.lt/VU:ELABAETD76894409&prefLang=en_US","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Pečiulienė, Inga,"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2020"]},{"key":"dc:publisher","label":"Institution","values":["Institutional Repository of Vilnius University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/doi/10.15388/vu.thesis.104","https://epublications.vu.lt/object/elaba:76894409/76894409.pdf"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dissertation ; hypoxia ; cancer ; pre-mRNA ; splicing"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["lit"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://repository.vu.lt/VU:ELABAETD76894409&prefLang=en_US"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Oncological diseases remain one of the unsolved problems of the entire society as the oncology-related morbidity and mortality has been systematically increasing. As pre-mRNA splicing is a highly important process in regulating gene expression, it is not surprising that changes in splicing have been observed in many different diseases. Unfortunately, the reasons for the expression of disease-associated mRNA isoforms and their regulatory factors have not been completely understood yet. This study describes the research of splicing factors and the expression of tumor-associated mRNA isoforms in healthy and tumor gastrointestinal organ tissues and tumors cell lines, and examines whether/how hypoxic microenvironment in tumor cell lines affects tumor-associated pre-mRNA splicing changes. Studies in this work have shown differences in the expression of SRSF1, SRSF2, U2AF65, U2AF35 and KHSRP splicing factors and tumor-associated Fas and Rac1 mRNA isoforms in tumor tissues and commonly used tumor cell lines. Our results indicate that reduced oxygen content in the cell environment (hypoxia) affects Fas pre-mRNA alternative splicing. The results obtained in this work provide valuable information about the processes initiating diseases in various systems."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Su onkologinėmis ligomis siejamas pre-iRNR splaisingas: splaisingo veiksnių ir hipoksinės mikroaplinkos tyrimas /","Pre-mRNA splicing associated with oncological diseases: a study of splicing factors and hypoxic microenvironment."]}]}],"canonical_facts":{"dc:creator":["Pečiulienė, Inga,"],"dc:date":["2020"],"dc:description":["Oncological diseases remain one of the unsolved problems of the entire society as the oncology-related morbidity and mortality has been systematically increasing. As pre-mRNA splicing is a highly important process in regulating gene expression, it is not surprising that changes in splicing have been observed in many different diseases. Unfortunately, the reasons for the expression of disease-associated mRNA isoforms and their regulatory factors have not been completely understood yet. This study describes the research of splicing factors and the expression of tumor-associated mRNA isoforms in healthy and tumor gastrointestinal organ tissues and tumors cell lines, and examines whether/how hypoxic microenvironment in tumor cell lines affects tumor-associated pre-mRNA splicing changes. Studies in this work have shown differences in the expression of SRSF1, SRSF2, U2AF65, U2AF35 and KHSRP splicing factors and tumor-associated Fas and Rac1 mRNA isoforms in tumor tissues and commonly used tumor cell lines. Our results indicate that reduced oxygen content in the cell environment (hypoxia) affects Fas pre-mRNA alternative splicing. The results obtained in this work provide valuable information about the processes initiating diseases in various systems."],"dc:format":["application/pdf"],"dc:identifier":["https://repository.vu.lt/VU:ELABAETD76894409&prefLang=en_US"],"dc:language":["lit"],"dc:publisher":["Institutional Repository of Vilnius University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/doi/10.15388/vu.thesis.104","https://epublications.vu.lt/object/elaba:76894409/76894409.pdf"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:subject":["dissertation ; hypoxia ; cancer ; pre-mRNA ; splicing"],"dc:title":["Su onkologinėmis ligomis siejamas pre-iRNR splaisingas: splaisingo veiksnių ir hipoksinės mikroaplinkos tyrimas /","Pre-mRNA splicing associated with oncological diseases: a study of splicing factors and hypoxic microenvironment."],"dc:type":["info:eu-repo/semantics/doctoralThesis"]},"updated_at":"2026-07-24T05:55:48Z"}