{"id":{"repo_id":"vilnius","oai_identifier":"oai:vu.lt:elaba:1916546"},"canonical_url":"https://search.dev.ndltd.org/etd/vilnius/oai:vu.lt:elaba:1916546","repository":{"repo_id":"vilnius","name":"Vilnius University","base_url":"https://epublications.vu.lt/oai"},"display":{"title":"Endoribonukleazių poveikio ankstyviesiems bakteriofago T4 transkriptams tyrimas /","abstract":"Phage T4 has developed a complex mechanism of ribonucleases control, which needs yet to be investigated. Apart from the impact of endoribonuclease RegB, no other effects of Escherichia coli- or phage-encoded proteins are known to be involved in the degradation of early mRNAs. This study has aimed to identify E. coli endoribonucleases that are involved in secondary processing in RegB-cleaved T4 mRNAs and to determine what phage T4-encoded factors affect the activity of these enzymes. We have shown that the endonucleolytic events at secondary sites of RegB-processed transcripts involve RNases G and E. The RNase G appears to be the main ribonuclease that cleaves all known secondary targets. Moreover, the revealed targets are the first RNase G targets identified in the bacteriophage T4 mRNA. This study has revealed that RNase G can be covalently modified during the infection cycle of bacteriophage T4. However, such modifications do not affect its activity related to the origin of secondary cuts in RegB-processed T4 mRNA. Another important finding is that T4K10 phage encodes defective polynucleotidkinase (PNK). In this study, we have shown that the G14D mutation of phage T4K10 PNK impairs 5'-kinase activity in vivo, as well as in vitro, and leads to the diminished processing of RegB-cleaved transcripts. This study has revealed that both, the T4 RNase RegB- and PNK-mediated activity of the E. coli RNases E and G is designed to accelerate degradation of phage T4 early transcripts.","abstract_html":"Phage T4 has developed a complex mechanism of ribonucleases control, which needs yet to be investigated. Apart from the impact of endoribonuclease RegB, no other effects of Escherichia coli- or phage-encoded proteins are known to be involved in the degradation of early mRNAs. This study has aimed to identify E. coli endoribonucleases that are involved in secondary processing in RegB-cleaved T4 mRNAs and to determine what phage T4-encoded factors affect the activity of these enzymes. We have shown that the endonucleolytic events at secondary sites of RegB-processed transcripts involve RNases G and E. The RNase G appears to be the main ribonuclease that cleaves all known secondary targets. Moreover, the revealed targets are the first RNase G targets identified in the bacteriophage T4 mRNA. This study has revealed that RNase G can be covalently modified during the infection cycle of bacteriophage T4. However, such modifications do not affect its activity related to the origin of secondary cuts in RegB-processed T4 mRNA. Another important finding is that T4K10 phage encodes defective polynucleotidkinase (PNK). In this study, we have shown that the G14D mutation of phage T4K10 PNK impairs 5'-kinase activity in vivo, as well as in vitro, and leads to the diminished processing of RegB-cleaved transcripts. This study has revealed that both, the T4 RNase RegB- and PNK-mediated activity of the E. coli RNases E and G is designed to accelerate degradation of phage T4 early transcripts.","abstract_has_math":false,"creators":["Strazdaitė Žielienė, Živilė,"],"institution":"Institutional Repository of Vilnius University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Truncaitė, Lidija"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013","date_published":"2013","updated_at":"2026-07-24T05:55:31Z","subjects":["Bacteriophage T4 ; RNase G ; RegB ; secondary processing ; PNK."],"languages":["lit"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://repository.vu.lt/VU:ELABAETD1916546&prefLang=en_US","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Truncaitė, Lidija"]},{"key":"dc:creator","label":"Author","values":["Strazdaitė Žielienė, Živilė,"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013"]},{"key":"dc:publisher","label":"Institution","values":["Institutional Repository of Vilnius University"]},{"key":"dc:relation","label":"Dc Relation","values":["https://epublications.vu.lt/object/elaba:1916546/1916546.pdf"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bacteriophage T4 ; RNase G ; RegB ; secondary processing ; PNK."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["lit"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://repository.vu.lt/VU:ELABAETD1916546&prefLang=en_US"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Phage T4 has developed a complex mechanism of ribonucleases control, which needs yet to be investigated. Apart from the impact of endoribonuclease RegB, no other effects of Escherichia coli- or phage-encoded proteins are known to be involved in the degradation of early mRNAs. This study has aimed to identify E. coli endoribonucleases that are involved in secondary processing in RegB-cleaved T4 mRNAs and to determine what phage T4-encoded factors affect the activity of these enzymes. We have shown that the endonucleolytic events at secondary sites of RegB-processed transcripts involve RNases G and E. The RNase G appears to be the main ribonuclease that cleaves all known secondary targets. Moreover, the revealed targets are the first RNase G targets identified in the bacteriophage T4 mRNA. This study has revealed that RNase G can be covalently modified during the infection cycle of bacteriophage T4. However, such modifications do not affect its activity related to the origin of secondary cuts in RegB-processed T4 mRNA. Another important finding is that T4K10 phage encodes defective polynucleotidkinase (PNK). In this study, we have shown that the G14D mutation of phage T4K10 PNK impairs 5'-kinase activity in vivo, as well as in vitro, and leads to the diminished processing of RegB-cleaved transcripts. This study has revealed that both, the T4 RNase RegB- and PNK-mediated activity of the E. coli RNases E and G is designed to accelerate degradation of phage T4 early transcripts."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Endoribonukleazių poveikio ankstyviesiems bakteriofago T4 transkriptams tyrimas /","Investigation of the impact of endoribonucleases on bacteriophage T4 early transcripts."]}]}],"canonical_facts":{"dc:contributor":["Truncaitė, Lidija"],"dc:creator":["Strazdaitė Žielienė, Živilė,"],"dc:date":["2013"],"dc:description":["Phage T4 has developed a complex mechanism of ribonucleases control, which needs yet to be investigated. Apart from the impact of endoribonuclease RegB, no other effects of Escherichia coli- or phage-encoded proteins are known to be involved in the degradation of early mRNAs. This study has aimed to identify E. coli endoribonucleases that are involved in secondary processing in RegB-cleaved T4 mRNAs and to determine what phage T4-encoded factors affect the activity of these enzymes. We have shown that the endonucleolytic events at secondary sites of RegB-processed transcripts involve RNases G and E. The RNase G appears to be the main ribonuclease that cleaves all known secondary targets. Moreover, the revealed targets are the first RNase G targets identified in the bacteriophage T4 mRNA. This study has revealed that RNase G can be covalently modified during the infection cycle of bacteriophage T4. However, such modifications do not affect its activity related to the origin of secondary cuts in RegB-processed T4 mRNA. Another important finding is that T4K10 phage encodes defective polynucleotidkinase (PNK). In this study, we have shown that the G14D mutation of phage T4K10 PNK impairs 5'-kinase activity in vivo, as well as in vitro, and leads to the diminished processing of RegB-cleaved transcripts. This study has revealed that both, the T4 RNase RegB- and PNK-mediated activity of the E. coli RNases E and G is designed to accelerate degradation of phage T4 early transcripts."],"dc:format":["application/pdf"],"dc:identifier":["https://repository.vu.lt/VU:ELABAETD1916546&prefLang=en_US"],"dc:language":["lit"],"dc:publisher":["Institutional Repository of Vilnius University"],"dc:relation":["https://epublications.vu.lt/object/elaba:1916546/1916546.pdf"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:subject":["Bacteriophage T4 ; RNase G ; RegB ; secondary processing ; PNK."],"dc:title":["Endoribonukleazių poveikio ankstyviesiems bakteriofago T4 transkriptams tyrimas /","Investigation of the impact of endoribonucleases on bacteriophage T4 early transcripts."],"dc:type":["info:eu-repo/semantics/doctoralThesis"]},"updated_at":"2026-07-24T05:55:31Z"}