{"id":{"repo_id":"vilnius","oai_identifier":"oai:vu.lt:elaba:16941432"},"canonical_url":"https://search.dev.ndltd.org/etd/vilnius/oai:vu.lt:elaba:16941432","repository":{"repo_id":"vilnius","name":"Vilnius University","base_url":"https://epublications.vu.lt/oai"},"display":{"title":"Identification and enantioselective determination of protein amino acids in fertilizers by hydrophilic interaction and chiral chromatography coupled to tandem mass spectrometry /","abstract":"Hydrolyzed protein fertilizers (HPF) are alternative nitrogen source for plants used in agriculture. The quality of HPF products is determined by quantity and enantiomeric purity of present protein amino acids in HPF product. High performance liquid chromatography coupled (HPLC) to tandem mass spectrometry (MS/MS) was involved for amino acids identification and enantioselective quantification studies. Direct amino acids identification assay in HPF samples was developed in hydrophilic interaction chromatography (HILIC) mode with tandem MS/MS detection. The developed method delivers fast analysis and superior specificity. Enantioselective HPLC on a glycopeptide antibiotic teicoplanin-based chiral stationary phase coupled to tandem mass spectrometry was developed for fast and reliable enantioseparation and determination of protein amino acids in HPF samples. Under optimized conditions, the majority (15 of 19) of D/L-amino acid pairs were resolved with a resolution factor (Rs) higher than 1.5 with a run time of 15 min. The method was validated in terms of linearity, limits of detection, limits of quantitation, precision, and accuracy. The developed method was successfully applied for determination of the free amino acid enantiomers in five commercially available hydrolyzed protein fertilizer samples.","abstract_html":"Hydrolyzed protein fertilizers (HPF) are alternative nitrogen source for plants used in agriculture. The quality of HPF products is determined by quantity and enantiomeric purity of present protein amino acids in HPF product. High performance liquid chromatography coupled (HPLC) to tandem mass spectrometry (MS/MS) was involved for amino acids identification and enantioselective quantification studies. Direct amino acids identification assay in HPF samples was developed in hydrophilic interaction chromatography (HILIC) mode with tandem MS/MS detection. The developed method delivers fast analysis and superior specificity. Enantioselective HPLC on a glycopeptide antibiotic teicoplanin-based chiral stationary phase coupled to tandem mass spectrometry was developed for fast and reliable enantioseparation and determination of protein amino acids in HPF samples. Under optimized conditions, the majority (15 of 19) of D/L-amino acid pairs were resolved with a resolution factor (Rs) higher than 1.5 with a run time of 15 min. The method was validated in terms of linearity, limits of detection, limits of quantitation, precision, and accuracy. The developed method was successfully applied for determination of the free amino acid enantiomers in five commercially available hydrolyzed protein fertilizer samples.","abstract_has_math":false,"creators":["Taujenis, Lukas,"],"institution":"Institutional Repository of Vilnius University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Padarauskas, Audrius"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016","date_published":"2016","updated_at":"2026-07-24T05:55:44Z","subjects":["HILIC ; chiral chromatography ; tandem mass spectrometry ; amino acids ; fertilizers"],"languages":["eng"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://repository.vu.lt/VU:ELABAETD16941432&prefLang=en_US","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Padarauskas, Audrius"]},{"key":"dc:creator","label":"Author","values":["Taujenis, Lukas,"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016"]},{"key":"dc:publisher","label":"Institution","values":["Institutional Repository of Vilnius University"]},{"key":"dc:relation","label":"Dc Relation","values":["https://epublications.vu.lt/object/elaba:16941432/16941432.pdf"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["HILIC ; chiral chromatography ; tandem mass spectrometry ; amino acids ; fertilizers"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://repository.vu.lt/VU:ELABAETD16941432&prefLang=en_US"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Hydrolyzed protein fertilizers (HPF) are alternative nitrogen source for plants used in agriculture. The quality of HPF products is determined by quantity and enantiomeric purity of present protein amino acids in HPF product. High performance liquid chromatography coupled (HPLC) to tandem mass spectrometry (MS/MS) was involved for amino acids identification and enantioselective quantification studies. Direct amino acids identification assay in HPF samples was developed in hydrophilic interaction chromatography (HILIC) mode with tandem MS/MS detection. The developed method delivers fast analysis and superior specificity. Enantioselective HPLC on a glycopeptide antibiotic teicoplanin-based chiral stationary phase coupled to tandem mass spectrometry was developed for fast and reliable enantioseparation and determination of protein amino acids in HPF samples. Under optimized conditions, the majority (15 of 19) of D/L-amino acid pairs were resolved with a resolution factor (Rs) higher than 1.5 with a run time of 15 min. The method was validated in terms of linearity, limits of detection, limits of quantitation, precision, and accuracy. The developed method was successfully applied for determination of the free amino acid enantiomers in five commercially available hydrolyzed protein fertilizer samples."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Identification and enantioselective determination of protein amino acids in fertilizers by hydrophilic interaction and chiral chromatography coupled to tandem mass spectrometry /","Baltymų aminorūgščių identifikavimas ir stereoselektyvus nustatymas trąšose hidrofilinės sąveikos ir chiralinės skysčių chromatografijos - tandeminės masių spektrometrijos metodais."]}]}],"canonical_facts":{"dc:contributor":["Padarauskas, Audrius"],"dc:creator":["Taujenis, Lukas,"],"dc:date":["2016"],"dc:description":["Hydrolyzed protein fertilizers (HPF) are alternative nitrogen source for plants used in agriculture. The quality of HPF products is determined by quantity and enantiomeric purity of present protein amino acids in HPF product. High performance liquid chromatography coupled (HPLC) to tandem mass spectrometry (MS/MS) was involved for amino acids identification and enantioselective quantification studies. Direct amino acids identification assay in HPF samples was developed in hydrophilic interaction chromatography (HILIC) mode with tandem MS/MS detection. The developed method delivers fast analysis and superior specificity. Enantioselective HPLC on a glycopeptide antibiotic teicoplanin-based chiral stationary phase coupled to tandem mass spectrometry was developed for fast and reliable enantioseparation and determination of protein amino acids in HPF samples. Under optimized conditions, the majority (15 of 19) of D/L-amino acid pairs were resolved with a resolution factor (Rs) higher than 1.5 with a run time of 15 min. The method was validated in terms of linearity, limits of detection, limits of quantitation, precision, and accuracy. The developed method was successfully applied for determination of the free amino acid enantiomers in five commercially available hydrolyzed protein fertilizer samples."],"dc:format":["application/pdf"],"dc:identifier":["https://repository.vu.lt/VU:ELABAETD16941432&prefLang=en_US"],"dc:language":["eng"],"dc:publisher":["Institutional Repository of Vilnius University"],"dc:relation":["https://epublications.vu.lt/object/elaba:16941432/16941432.pdf"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:subject":["HILIC ; chiral chromatography ; tandem mass spectrometry ; amino acids ; fertilizers"],"dc:title":["Identification and enantioselective determination of protein amino acids in fertilizers by hydrophilic interaction and chiral chromatography coupled to tandem mass spectrometry /","Baltymų aminorūgščių identifikavimas ir stereoselektyvus nustatymas trąšose hidrofilinės sąveikos ir chiralinės skysčių chromatografijos - tandeminės masių spektrometrijos metodais."],"dc:type":["info:eu-repo/semantics/doctoralThesis"]},"updated_at":"2026-07-24T05:55:44Z"}