{"id":{"repo_id":"vilnius","oai_identifier":"oai:vu.lt:elaba:112886347"},"canonical_url":"https://search.dev.ndltd.org/etd/vilnius/oai:vu.lt:elaba:112886347","repository":{"repo_id":"vilnius","name":"Vilnius University","base_url":"https://epublications.vu.lt/oai"},"display":{"title":"Scanning electrochemical microscopy for the investigation of biosensors /","abstract":"Saccharomyces cerevisiae cells were electrochemically investigated in a double redox mediator system with varying lipophilic mediators and optimal parameters were found. The yeast cells were later modified with a conductive polymer - polypyrrole and multi walled carbon nanotubes for evaluating changes in the charge transfer. These modified cells were examined with atomic force microscopy, scanning electron microscopy, cyclic voltammetry and scanning electrochemical microscopy after the immobilization on a carbon felt electrode. The in-situ microorganism assisted polymerization with Aspergillus niger and Rhizoctania sp. cells was studied amperometrically by immobilizing them on a graphite rod and using it as a working electrode. The modification was also tested by immobilizing the cells on a Petri dish and evaluated by the Scanning electrochemical microscope. The other line of experiments involved the detection of the human growth hormone by electrochemical means. The antibodies against the human growth hormone were labelled with glucose oxidase which was detectable with the scanning electrochemical microscope in a solution containing glucose. The human growth hormone was detected in a non-competitive sandwich mode immunoassay with the glucose oxidase labelled human growth antibody in a solution containing glucose and potassium ferricyanide as the redox mediator in a substrate generation – tip collection scanning electrochemical microscopy mode.","abstract_html":"Saccharomyces cerevisiae cells were electrochemically investigated in a double redox mediator system with varying lipophilic mediators and optimal parameters were found. The yeast cells were later modified with a conductive polymer - polypyrrole and multi walled carbon nanotubes for evaluating changes in the charge transfer. These modified cells were examined with atomic force microscopy, scanning electron microscopy, cyclic voltammetry and scanning electrochemical microscopy after the immobilization on a carbon felt electrode. The in-situ microorganism assisted polymerization with Aspergillus niger and Rhizoctania sp. cells was studied amperometrically by immobilizing them on a graphite rod and using it as a working electrode. The modification was also tested by immobilizing the cells on a Petri dish and evaluated by the Scanning electrochemical microscope. The other line of experiments involved the detection of the human growth hormone by electrochemical means. The antibodies against the human growth hormone were labelled with glucose oxidase which was detectable with the scanning electrochemical microscope in a solution containing glucose. The human growth hormone was detected in a non-competitive sandwich mode immunoassay with the glucose oxidase labelled human growth antibody in a solution containing glucose and potassium ferricyanide as the redox mediator in a substrate generation – tip collection scanning electrochemical microscopy mode.","abstract_has_math":false,"creators":["Kisieliūtė, Aura,"],"institution":"Institutional Repository of Vilnius University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Ramanavičius, Arūnas"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021","date_published":"2021","updated_at":"2026-07-24T05:55:35Z","subjects":["Scanning electrochemical microscopy ; biosensors ; redox ; immune sensors ; polypyrrole"],"languages":["eng"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://repository.vu.lt/VU:ELABAETD112886347&prefLang=en_US","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ramanavičius, Arūnas"]},{"key":"dc:creator","label":"Author","values":["Kisieliūtė, Aura,"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021"]},{"key":"dc:publisher","label":"Institution","values":["Institutional Repository of Vilnius University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/doi/10.15388/vu.thesis.256","https://epublications.vu.lt/object/elaba:112886347/112886347.pdf"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Scanning electrochemical microscopy ; biosensors ; redox ; immune sensors ; polypyrrole"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://repository.vu.lt/VU:ELABAETD112886347&prefLang=en_US"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Saccharomyces cerevisiae cells were electrochemically investigated in a double redox mediator system with varying lipophilic mediators and optimal parameters were found. The yeast cells were later modified with a conductive polymer - polypyrrole and multi walled carbon nanotubes for evaluating changes in the charge transfer. These modified cells were examined with atomic force microscopy, scanning electron microscopy, cyclic voltammetry and scanning electrochemical microscopy after the immobilization on a carbon felt electrode. The in-situ microorganism assisted polymerization with Aspergillus niger and Rhizoctania sp. cells was studied amperometrically by immobilizing them on a graphite rod and using it as a working electrode. The modification was also tested by immobilizing the cells on a Petri dish and evaluated by the Scanning electrochemical microscope. The other line of experiments involved the detection of the human growth hormone by electrochemical means. The antibodies against the human growth hormone were labelled with glucose oxidase which was detectable with the scanning electrochemical microscope in a solution containing glucose. The human growth hormone was detected in a non-competitive sandwich mode immunoassay with the glucose oxidase labelled human growth antibody in a solution containing glucose and potassium ferricyanide as the redox mediator in a substrate generation – tip collection scanning electrochemical microscopy mode."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Scanning electrochemical microscopy for the investigation of biosensors /","Skenuojančios elektrocheminės mikroskopijos taikymas biologinių jutiklių tyrimuose."]}]}],"canonical_facts":{"dc:contributor":["Ramanavičius, Arūnas"],"dc:creator":["Kisieliūtė, Aura,"],"dc:date":["2021"],"dc:description":["Saccharomyces cerevisiae cells were electrochemically investigated in a double redox mediator system with varying lipophilic mediators and optimal parameters were found. The yeast cells were later modified with a conductive polymer - polypyrrole and multi walled carbon nanotubes for evaluating changes in the charge transfer. These modified cells were examined with atomic force microscopy, scanning electron microscopy, cyclic voltammetry and scanning electrochemical microscopy after the immobilization on a carbon felt electrode. The in-situ microorganism assisted polymerization with Aspergillus niger and Rhizoctania sp. cells was studied amperometrically by immobilizing them on a graphite rod and using it as a working electrode. The modification was also tested by immobilizing the cells on a Petri dish and evaluated by the Scanning electrochemical microscope. The other line of experiments involved the detection of the human growth hormone by electrochemical means. The antibodies against the human growth hormone were labelled with glucose oxidase which was detectable with the scanning electrochemical microscope in a solution containing glucose. The human growth hormone was detected in a non-competitive sandwich mode immunoassay with the glucose oxidase labelled human growth antibody in a solution containing glucose and potassium ferricyanide as the redox mediator in a substrate generation – tip collection scanning electrochemical microscopy mode."],"dc:format":["application/pdf"],"dc:identifier":["https://repository.vu.lt/VU:ELABAETD112886347&prefLang=en_US"],"dc:language":["eng"],"dc:publisher":["Institutional Repository of Vilnius University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/doi/10.15388/vu.thesis.256","https://epublications.vu.lt/object/elaba:112886347/112886347.pdf"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:subject":["Scanning electrochemical microscopy ; biosensors ; redox ; immune sensors ; polypyrrole"],"dc:title":["Scanning electrochemical microscopy for the investigation of biosensors /","Skenuojančios elektrocheminės mikroskopijos taikymas biologinių jutiklių tyrimuose."],"dc:type":["info:eu-repo/semantics/doctoralThesis"]},"updated_at":"2026-07-24T05:55:35Z"}