https://search.dev.ndltd.org/etd/venda/oai:univendspace.univen.ac.za:123456789/2703
type: CreativeWork
name: Pharmacogenetic analysis of admc polymorphisms affecting the efficacy of TB treatment in tuberculosis patients from Tanzania and Uganda, and pharmalogical evaluation of potent medicinal plant extracts used in the treatment of tuberculosis
description: Background: Despite the availability of antibiotics which can cure tuberculosis (TB), it remains the leading cause of death from a single infectious disease worldwide. Recent research has shown that the way anti-tuberculous drugs are processed by the body (pharmacokinetics) and their effects on the disease (pharmacodynamics) play a crucial role in determining the success of treatment. To find new treatments which are less harmful than current regimens, medicinal plants are being explored for their potential to produce compounds with anti-TB properties. Therefore, it was hypothesised that the effectiveness of anti-TB drugs may also depend on an individual's ADME polymorphisms. Aim: This study was aimed at evaluating the pharmacogenetics of Tanzanian and Ugandan tuberculosis patients, and pharmacological effects of medicinal plants [S. brachypetala (MMV004), R. caffra (MMV003), S. molle (MMV005), Z. mucronata (MMV002) and S. petersiana (MMV001)], used for the treatment of symptoms related to tuberculosis infection. Methodology: Saliva samples were collected from the study participants at treatment initiatiion using Genotek tissue collection kit. DNA Genotek extraction procedure was used to extact DNA from the samples and used in evaluation of custom-designed ADME targets for polymorphisms using real-time polymerase chain reaction on 96 well plates. The pharmacogenetic analysis included target genes responsible for the ADME of isoniazid, rifampin, pyrazinamide, and levofloxacin/moxifloxacin, which are currently the most widely used anti-TB drugs, including medicinal plants. For the pharmacological evaluation of the medicinal plants, plant materials were dried and ground it into fine powder and extracted with solvents of varying polarity. The phytochemical profile of each plant species was assessed using Thin Layer Chromatography (TLC) plates developed in three solvent systems - benzene/ethanol/ammonium hydroxide (BEA), chloroform/ethyl acetate/formic acid (CEF), and et…
abstract: Background: Despite the availability of antibiotics which can cure tuberculosis (TB), it remains the leading cause of death from a single infectious disease worldwide. Recent research has shown that the way anti-tuberculous drugs are processed by the body (pharmacokinetics) and their effects on the disease (pharmacodynamics) play a crucial role in determining the success of treatment. To find new treatments which are less harmful than current regimens, medicinal plants are being explored for their potential to produce compounds with anti-TB properties. Therefore, it was hypothesised that the effectiveness of anti-TB drugs may also depend on an individual's ADME polymorphisms. Aim: This study was aimed at evaluating the pharmacogenetics of Tanzanian and Ugandan tuberculosis patients, and pharmacological effects of medicinal plants [S. brachypetala (MMV004), R. caffra (MMV003), S. molle (MMV005), Z. mucronata (MMV002) and S. petersiana (MMV001)], used for the treatment of symptoms related to tuberculosis infection. Methodology: Saliva samples were collected from the study participants at treatment initiatiion using Genotek tissue collection kit. DNA Genotek extraction procedure was used to extact DNA from the samples and used in evaluation of custom-designed ADME targets for polymorphisms using real-time polymerase chain reaction on 96 well plates. The pharmacogenetic analysis included target genes responsible for the ADME of isoniazid, rifampin, pyrazinamide, and levofloxacin/moxifloxacin, which are currently the most widely used anti-TB drugs, including medicinal plants. For the pharmacological evaluation of the medicinal plants, plant materials were dried and ground it into fine powder and extracted with solvents of varying polarity. The phytochemical profile of each plant species was assessed using Thin Layer Chromatography (TLC) plates developed in three solvent systems - benzene/ethanol/ammonium hydroxide (BEA), chloroform/ethyl acetate/formic acid (CEF), and et…
author: Masiphephethu, M. V.
language: en
date published: 2024-05-17
date modified: 2026-07-27T21:57:55Z
provider: University of Venda
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Venda