Synthesis and Characterisation of Metallic Nanoparticles from Medicinal Plant Extracts and their Application in combating Antibiotic-Resistant Bacterial Pathogens
type: CreativeWork
description: Background: The rise of antibiotic-resistant (ABR) bacterial pathogens is a major global health concern, especially in the case of respiratory tract infections (RTIs), which can make treatment more difficult. According to statistics, more than 2 million lives were lost due to ABR bacterial infections in 2019, highlighting the seriousness of the situation. This study aims to propose a novel approach to combating ABR bacteria by synthesising metallic nanoparticles (MNPs) from medicinal plants. Integrating nanoparticle-based strategies with medicinal plants demonstrates considerable promise in addressing RTIs. Methodology: The present study investigated the extracts of Spirostachys africana (S. africana), a medicinal plant traditionally used by healers to treat various infections. Extracts were prepared using methanol, ethanol, acetone, and distilled water. A comprehensive approach was employed to synthesise and characterise silver (SA-AgNPs) and gold (SAAuNPs) nanoparticles from S. africana extracts. Methods of characterisation included Ultraviolet-Visible spectrophotometry (UV-Vis), X-ray diffraction (XRD), Dynamic light scattering (DLS), Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), and Liquid chromatography and mass spectroscopy (LC-MS). The antimicrobial activity of the nanoparticles and extracts was tested against the World Health Organization (WHO) ABR priority pathogens using agar well diffusion and microdilution assays. We further, assessed the cytotoxicity, anti-inflammatory, and antioxidant activities using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Griess assays, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, respectively. Results: The UV-Vis analysis revealed surface plasmon resonance (SPR) peaks at 480 nm and 541 nm, indicating the formation of SA-AgNPs and SA-AuNPs, respectively. XRD showed face-centred cubic structures with crystalline sizes ranging from 9–19 nm for SA-AgNPs and 9–10 …
abstract: Background: The rise of antibiotic-resistant (ABR) bacterial pathogens is a major global health concern, especially in the case of respiratory tract infections (RTIs), which can make treatment more difficult. According to statistics, more than 2 million lives were lost due to ABR bacterial infections in 2019, highlighting the seriousness of the situation. This study aims to propose a novel approach to combating ABR bacteria by synthesising metallic nanoparticles (MNPs) from medicinal plants. Integrating nanoparticle-based strategies with medicinal plants demonstrates considerable promise in addressing RTIs. Methodology: The present study investigated the extracts of Spirostachys africana (S. africana), a medicinal plant traditionally used by healers to treat various infections. Extracts were prepared using methanol, ethanol, acetone, and distilled water. A comprehensive approach was employed to synthesise and characterise silver (SA-AgNPs) and gold (SAAuNPs) nanoparticles from S. africana extracts. Methods of characterisation included Ultraviolet-Visible spectrophotometry (UV-Vis), X-ray diffraction (XRD), Dynamic light scattering (DLS), Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), and Liquid chromatography and mass spectroscopy (LC-MS). The antimicrobial activity of the nanoparticles and extracts was tested against the World Health Organization (WHO) ABR priority pathogens using agar well diffusion and microdilution assays. We further, assessed the cytotoxicity, anti-inflammatory, and antioxidant activities using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Griess assays, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, respectively. Results: The UV-Vis analysis revealed surface plasmon resonance (SPR) peaks at 480 nm and 541 nm, indicating the formation of SA-AgNPs and SA-AuNPs, respectively. XRD showed face-centred cubic structures with crystalline sizes ranging from 9–19 nm for SA-AgNPs and 9–10 …
author: Nemaguvhuni, Murunwa Felicia
language: en
keywords: Antibiotic -resistant bacteria, Respiratory tract infections, SpiroStachys Africana, Metallic nanoparticles, Antimicrobial activity, Cytotoxicity, Anti-inflammatory activity, UCTD
date published: 2024-09-06
date modified: 2026-07-27T21:57:20Z
provider: University of Venda
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Venda