{"id":{"repo_id":"vcu","oai_identifier":"oai:scholarscompass.vcu.edu:etd-2438"},"canonical_url":"https://search.dev.ndltd.org/etd/vcu/oai:scholarscompass.vcu.edu:etd-2438","repository":{"repo_id":"vcu","name":"Virginia Commonwealth University","base_url":"https://scholarscompass.vcu.edu/do/oai/"},"display":{"title":"Inhibition of Margination and Diapedesis of Neutrophils by Protein Synthesis Blockade","abstract":"Acute Respiratory Distress Syndrome (ARDS), an age-old clinical problem facing the Emergency Department and Intensive Care Units of all health systems, is a common debilitating lung condition consequent upon severe systemic inflammation. Although several studies have gone into understanding the epidemiology and pathogenesis of the disease thus making way for new advances in treatment strategies, there seems to be no known study tailored to its prevention. Neutrophil extravasation within the tissues during inflammation is the hallmark of this syndrome. Our study sought to block excessive neutrophil infiltration by inhibiting the biosynthesis of some essential proteins necessary for the process. In this initial study, neutrophil transmigration was successfully reduced by 66% using protein synthesis inhibitors, a combination of puromycin and anisomycin. Our strategy, if fine-tuned, could form the basis of a new clinical strategy for the prevention of ARDS.","abstract_html":"Acute Respiratory Distress Syndrome (ARDS), an age-old clinical problem facing the Emergency Department and Intensive Care Units of all health systems, is a common debilitating lung condition consequent upon severe systemic inflammation. Although several studies have gone into understanding the epidemiology and pathogenesis of the disease thus making way for new advances in treatment strategies, there seems to be no known study tailored to its prevention. Neutrophil extravasation within the tissues during inflammation is the hallmark of this syndrome. Our study sought to block excessive neutrophil infiltration by inhibiting the biosynthesis of some essential proteins necessary for the process. In this initial study, neutrophil transmigration was successfully reduced by 66% using protein synthesis inhibitors, a combination of puromycin and anisomycin. Our strategy, if fine-tuned, could form the basis of a new clinical strategy for the prevention of ARDS.","abstract_has_math":false,"creators":["Acquah, Phyllis V."],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Dr. Sarah Spiegal"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006-01-01T08:00:00Z","date_published":"2006-01-01T08:00:00Z","updated_at":"2026-07-24T05:55:25Z","subjects":["respiratory","immune response","lung disease","ARDS","Biochemistry, Biophysics, and Structural Biology","Life Sciences"],"languages":[],"rights":["© The Author"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarscompass.vcu.edu/etd/1439"],"render_values":[{"text":"https://scholarscompass.vcu.edu/etd/1439","href":"https://scholarscompass.vcu.edu/etd/1439","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25772/K9TX-DX77","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Sarah Spiegal"]},{"key":"dc:creator","label":"Author","values":["Acquah, Phyllis V."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-09T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["respiratory","immune response","lung disease","ARDS","Biochemistry, Biophysics, and Structural Biology","Life Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© The Author"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.25772/K9TX-DX77","https://scholarscompass.vcu.edu/etd/1439"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Acute Respiratory Distress Syndrome (ARDS), an age-old clinical problem facing the Emergency Department and Intensive Care Units of all health systems, is a common debilitating lung condition consequent upon severe systemic inflammation. Although several studies have gone into understanding the epidemiology and pathogenesis of the disease thus making way for new advances in treatment strategies, there seems to be no known study tailored to its prevention. Neutrophil extravasation within the tissues during inflammation is the hallmark of this syndrome. Our study sought to block excessive neutrophil infiltration by inhibiting the biosynthesis of some essential proteins necessary for the process. In this initial study, neutrophil transmigration was successfully reduced by 66% using protein synthesis inhibitors, a combination of puromycin and anisomycin. Our strategy, if fine-tuned, could form the basis of a new clinical strategy for the prevention of ARDS."]},{"key":"dc:title","label":"Title","values":["Inhibition of Margination and Diapedesis of Neutrophils by Protein Synthesis Blockade"]}]}],"canonical_facts":{"dc:contributor":["Dr. Sarah Spiegal"],"dc:creator":["Acquah, Phyllis V."],"dc:date.available":["2014-07-09T07:00:00Z"],"dc:description.abstract":["Acute Respiratory Distress Syndrome (ARDS), an age-old clinical problem facing the Emergency Department and Intensive Care Units of all health systems, is a common debilitating lung condition consequent upon severe systemic inflammation. Although several studies have gone into understanding the epidemiology and pathogenesis of the disease thus making way for new advances in treatment strategies, there seems to be no known study tailored to its prevention. Neutrophil extravasation within the tissues during inflammation is the hallmark of this syndrome. Our study sought to block excessive neutrophil infiltration by inhibiting the biosynthesis of some essential proteins necessary for the process. In this initial study, neutrophil transmigration was successfully reduced by 66% using protein synthesis inhibitors, a combination of puromycin and anisomycin. Our strategy, if fine-tuned, could form the basis of a new clinical strategy for the prevention of ARDS."],"dc:identifier":["https://doi.org/10.25772/K9TX-DX77","https://scholarscompass.vcu.edu/etd/1439"],"dc:rights":["© The Author"],"dc:subject":["respiratory","immune response","lung disease","ARDS","Biochemistry, Biophysics, and Structural Biology","Life Sciences"],"dc:title":["Inhibition of Margination and Diapedesis of Neutrophils by Protein Synthesis Blockade"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T05:55:25Z"}