{"id":{"repo_id":"vcu","oai_identifier":"oai:scholarscompass.vcu.edu:etd-2414"},"canonical_url":"https://search.dev.ndltd.org/etd/vcu/oai:scholarscompass.vcu.edu:etd-2414","repository":{"repo_id":"vcu","name":"Virginia Commonwealth University","base_url":"https://scholarscompass.vcu.edu/do/oai/"},"display":{"title":"Interleukin-10 Suppresses Mast Cell IgE Receptor Expression And Signaling In Vitro And In Vivo","abstract":"Background: Mast cells are known for their role in allergy, asthma, and systemic anaphylaxis, and have been shown to play a role in inflammatory disease. Interleukin-10 can regulate inflammatory responses both in vitro and in vivo, and may be a natural regulator of mast cell activation.Objective: To examine Interleukin-10 mediated regulation of FcεRI expression and related downstream signaling molecules, and to determine how this affects mast cell function in vitro and in vivo.Methods: Mast cell FcεRI expression was evaluated with and without IL-10 treatment in human lung and skin mast cells, and on peritoneal mast cells from mice overexpressing IL-10 via injection or a transgenic model. Mast cell function was evaluated by observing responses of IL-10 treated mice to passive systemic anaphylaxis.Results: Interleukin-10 inhibited FcεRI expression on mouse and human mast cells, both in vitro and in vivo. IL-10 also suppressed expression of the key signaling molecules Syk, Fyn, Akt and Stat5. Mice chronically overexpressing IL-10 had a reduced response to passive systemic anaphylaxis, indicating impaired mast cell activation.Conclusion: Interleukin-10 suppresses mast cell FcεRI expression in vitro and in vivo, and reduces IgE-mediated activation. The anti-inflammatory effects of IL-10 may relate to its suppression of critical signaling molecules.Clinical Implications: Interleukin-10 polymorphism is associated with increased IgE levels and incidence of atopic disease; hence IL-10 dysregulation may affect atopic etiology. Further, IL-10 therapy is a possible treatment for atopic allergy and asthma.","abstract_html":"Background: Mast cells are known for their role in allergy, asthma, and systemic anaphylaxis, and have been shown to play a role in inflammatory disease. Interleukin-10 can regulate inflammatory responses both in vitro and in vivo, and may be a natural regulator of mast cell activation.Objective: To examine Interleukin-10 mediated regulation of FcεRI expression and related downstream signaling molecules, and to determine how this affects mast cell function in vitro and in vivo.Methods: Mast cell FcεRI expression was evaluated with and without IL-10 treatment in human lung and skin mast cells, and on peritoneal mast cells from mice overexpressing IL-10 via injection or a transgenic model. Mast cell function was evaluated by observing responses of IL-10 treated mice to passive systemic anaphylaxis.Results: Interleukin-10 inhibited FcεRI expression on mouse and human mast cells, both in vitro and in vivo. IL-10 also suppressed expression of the key signaling molecules Syk, Fyn, Akt and Stat5. Mice chronically overexpressing IL-10 had a reduced response to passive systemic anaphylaxis, indicating impaired mast cell activation.Conclusion: Interleukin-10 suppresses mast cell FcεRI expression in vitro and in vivo, and reduces IgE-mediated activation. The anti-inflammatory effects of IL-10 may relate to its suppression of critical signaling molecules.Clinical Implications: Interleukin-10 polymorphism is associated with increased IgE levels and incidence of atopic disease; hence IL-10 dysregulation may affect atopic etiology. Further, IL-10 therapy is a possible treatment for atopic allergy and asthma.","abstract_has_math":false,"creators":["Kennedy, Sarah B."],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Dr. John J. Ryan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007-01-01T08:00:00Z","date_published":"2007-01-01T08:00:00Z","updated_at":"2026-07-24T05:55:25Z","subjects":["IL-10","FcεRI","cell regulator","mast cell","Biology","Life Sciences"],"languages":[],"rights":["© The Author"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarscompass.vcu.edu/etd/1415"],"render_values":[{"text":"https://scholarscompass.vcu.edu/etd/1415","href":"https://scholarscompass.vcu.edu/etd/1415","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25772/0NEM-KJ14","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. John J. Ryan"]},{"key":"dc:creator","label":"Author","values":["Kennedy, Sarah B."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-09T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["IL-10","FcεRI","cell regulator","mast cell","Biology","Life Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© The Author"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.25772/0NEM-KJ14","https://scholarscompass.vcu.edu/etd/1415"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Background: Mast cells are known for their role in allergy, asthma, and systemic anaphylaxis, and have been shown to play a role in inflammatory disease. Interleukin-10 can regulate inflammatory responses both in vitro and in vivo, and may be a natural regulator of mast cell activation.Objective: To examine Interleukin-10 mediated regulation of FcεRI expression and related downstream signaling molecules, and to determine how this affects mast cell function in vitro and in vivo.Methods: Mast cell FcεRI expression was evaluated with and without IL-10 treatment in human lung and skin mast cells, and on peritoneal mast cells from mice overexpressing IL-10 via injection or a transgenic model. Mast cell function was evaluated by observing responses of IL-10 treated mice to passive systemic anaphylaxis.Results: Interleukin-10 inhibited FcεRI expression on mouse and human mast cells, both in vitro and in vivo. IL-10 also suppressed expression of the key signaling molecules Syk, Fyn, Akt and Stat5. Mice chronically overexpressing IL-10 had a reduced response to passive systemic anaphylaxis, indicating impaired mast cell activation.Conclusion: Interleukin-10 suppresses mast cell FcεRI expression in vitro and in vivo, and reduces IgE-mediated activation. The anti-inflammatory effects of IL-10 may relate to its suppression of critical signaling molecules.Clinical Implications: Interleukin-10 polymorphism is associated with increased IgE levels and incidence of atopic disease; hence IL-10 dysregulation may affect atopic etiology. Further, IL-10 therapy is a possible treatment for atopic allergy and asthma."]},{"key":"dc:title","label":"Title","values":["Interleukin-10 Suppresses Mast Cell IgE Receptor Expression And Signaling In Vitro And In Vivo"]}]}],"canonical_facts":{"dc:contributor":["Dr. John J. Ryan"],"dc:creator":["Kennedy, Sarah B."],"dc:date.available":["2014-07-09T07:00:00Z"],"dc:description.abstract":["Background: Mast cells are known for their role in allergy, asthma, and systemic anaphylaxis, and have been shown to play a role in inflammatory disease. Interleukin-10 can regulate inflammatory responses both in vitro and in vivo, and may be a natural regulator of mast cell activation.Objective: To examine Interleukin-10 mediated regulation of FcεRI expression and related downstream signaling molecules, and to determine how this affects mast cell function in vitro and in vivo.Methods: Mast cell FcεRI expression was evaluated with and without IL-10 treatment in human lung and skin mast cells, and on peritoneal mast cells from mice overexpressing IL-10 via injection or a transgenic model. Mast cell function was evaluated by observing responses of IL-10 treated mice to passive systemic anaphylaxis.Results: Interleukin-10 inhibited FcεRI expression on mouse and human mast cells, both in vitro and in vivo. IL-10 also suppressed expression of the key signaling molecules Syk, Fyn, Akt and Stat5. Mice chronically overexpressing IL-10 had a reduced response to passive systemic anaphylaxis, indicating impaired mast cell activation.Conclusion: Interleukin-10 suppresses mast cell FcεRI expression in vitro and in vivo, and reduces IgE-mediated activation. The anti-inflammatory effects of IL-10 may relate to its suppression of critical signaling molecules.Clinical Implications: Interleukin-10 polymorphism is associated with increased IgE levels and incidence of atopic disease; hence IL-10 dysregulation may affect atopic etiology. Further, IL-10 therapy is a possible treatment for atopic allergy and asthma."],"dc:identifier":["https://doi.org/10.25772/0NEM-KJ14","https://scholarscompass.vcu.edu/etd/1415"],"dc:rights":["© The Author"],"dc:subject":["IL-10","FcεRI","cell regulator","mast cell","Biology","Life Sciences"],"dc:title":["Interleukin-10 Suppresses Mast Cell IgE Receptor Expression And Signaling In Vitro And In Vivo"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T05:55:25Z"}