Cytokine and Chemokine Profiles in a Rat Model of Hemorrhagic Shock after Immuno-Modulation by Androstenetriol

Further understanding of the cellular and molecular mechanisms involved in traumatic injury, and how they are modulated during drug interaction, can facilitate novel treatment strategies for future trauma patients. We hypothesize that the pharmacological agent, Androstenetriol (AET), up regulates host immune response by modulating the continued expression of mediators, including cytokines. In a double-blinded experiment, rats were hemorrhaged, driven by volume or pressure conditions, then resuscitated with fluids and packed red blood cells following a subcutaneous injection of either vehicle or drug. Blood was collected at various time points and cytokine levels were determined by analyses with both multiplex and conventional ELISA assays. Both MCP-1 and GRO/KC increase in surviving animals; trauma increases IL-lα levels in rat plasma, whereas hemorrhage decreases IL-lα over time; IL-6 plasma levels measured 6 hours after hemorrhage may correlate with mortality; AET may act by mechanisms to modify specific TH1 cytokines (INF-y) to promote survival.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biochemistry

Year dc:date.available

2005

Author dc:creator

• Paccione, Kristin E

Contributors dc:contributor

• Dr. Robert F. Diegelman

• hemorrage
• shock
• AET
• traumatic injury
• Biochemistry, Biophysics, and Structural Biology
• Life Sciences

Statement dc:rights

• © The Author

Identifier

https://scholarscompass.vcu.edu/etd/1389

OAI identifier oai:identifier

oai:scholarscompass.vcu.edu:etd-2388