{"id":{"repo_id":"vcu","oai_identifier":"oai:scholarscompass.vcu.edu:etd-2126"},"canonical_url":"https://search.dev.ndltd.org/etd/vcu/oai:scholarscompass.vcu.edu:etd-2126","repository":{"repo_id":"vcu","name":"Virginia Commonwealth University","base_url":"https://scholarscompass.vcu.edu/do/oai/"},"display":{"title":"Autocrine Effects of Catecholamines on Macrophage Release of Interleukin-6 (IL-6)","abstract":"Effects of norepinephrine (NE) on macrophage cytokine release are complex because the cells have both α2 and β2 adrenergic receptors, which mediate opposing actions. Furthermore, β2-adrenergic agonists are reported to have both stimulatory and inhibitory effects on interleukin-6 (IL-6). This study was designed to clarify the autocrine role of macrophage-derived NE on IL-6 production in activated peritoneal macrophages. Effects of NE on IL-6 production in the RAW264.7 macrophage cell-line also were investigated. Treatment of activated peritoneal macrophages with endotoxin, the α2-adrenergic antagonists yohimbine or RS79948 revealed that the α2-adrenergic receptor mediates a stimulatory autocrine action of catecholamines on IL-6 production. When peritoneal macrophages were treated with the β2 antagonist ICI-118,551 (ICI), there was both inhibition and stimulation of IL-6. Treatment of RAW264.7 macrophages with high and low concentrations of NE and various concentrations of ICI provided evidence that the concentration of NE determines whether the β2-adrenergic receptor mediates stimulation or inhibition of IL-6 production.","abstract_html":"Effects of norepinephrine (NE) on macrophage cytokine release are complex because the cells have both α2 and β2 adrenergic receptors, which mediate opposing actions. Furthermore, β2-adrenergic agonists are reported to have both stimulatory and inhibitory effects on interleukin-6 (IL-6). This study was designed to clarify the autocrine role of macrophage-derived NE on IL-6 production in activated peritoneal macrophages. Effects of NE on IL-6 production in the RAW264.7 macrophage cell-line also were investigated. Treatment of activated peritoneal macrophages with endotoxin, the α2-adrenergic antagonists yohimbine or RS79948 revealed that the α2-adrenergic receptor mediates a stimulatory autocrine action of catecholamines on IL-6 production. When peritoneal macrophages were treated with the β2 antagonist ICI-118,551 (ICI), there was both inhibition and stimulation of IL-6. Treatment of RAW264.7 macrophages with high and low concentrations of NE and various concentrations of ICI provided evidence that the concentration of NE determines whether the β2-adrenergic receptor mediates stimulation or inhibition of IL-6 production.","abstract_has_math":false,"creators":["Poe, Shaunta D."],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Dr. Jennifer K. Stewart","Dr. Krista J. Fischer-Stenger"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006-01-01T08:00:00Z","date_published":"2006-01-01T08:00:00Z","updated_at":"2026-07-24T05:54:53Z","subjects":["peritoneal","norepinephrine","agonist","receptor","Biology","Life Sciences"],"languages":[],"rights":["© The Author"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarscompass.vcu.edu/etd/1127"],"render_values":[{"text":"https://scholarscompass.vcu.edu/etd/1127","href":"https://scholarscompass.vcu.edu/etd/1127","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25772/5VA8-0E08","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Jennifer K. Stewart","Dr. Krista J. 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Furthermore, β2-adrenergic agonists are reported to have both stimulatory and inhibitory effects on interleukin-6 (IL-6). This study was designed to clarify the autocrine role of macrophage-derived NE on IL-6 production in activated peritoneal macrophages. Effects of NE on IL-6 production in the RAW264.7 macrophage cell-line also were investigated. Treatment of activated peritoneal macrophages with endotoxin, the α2-adrenergic antagonists yohimbine or RS79948 revealed that the α2-adrenergic receptor mediates a stimulatory autocrine action of catecholamines on IL-6 production. When peritoneal macrophages were treated with the β2 antagonist ICI-118,551 (ICI), there was both inhibition and stimulation of IL-6. Treatment of RAW264.7 macrophages with high and low concentrations of NE and various concentrations of ICI provided evidence that the concentration of NE determines whether the β2-adrenergic receptor mediates stimulation or inhibition of IL-6 production."]},{"key":"dc:title","label":"Title","values":["Autocrine Effects of Catecholamines on Macrophage Release of Interleukin-6 (IL-6)"]}]}],"canonical_facts":{"dc:contributor":["Dr. Jennifer K. Stewart","Dr. Krista J. Fischer-Stenger"],"dc:creator":["Poe, Shaunta D."],"dc:date.available":["2014-07-09T07:00:00Z"],"dc:description.abstract":["Effects of norepinephrine (NE) on macrophage cytokine release are complex because the cells have both α2 and β2 adrenergic receptors, which mediate opposing actions. Furthermore, β2-adrenergic agonists are reported to have both stimulatory and inhibitory effects on interleukin-6 (IL-6). This study was designed to clarify the autocrine role of macrophage-derived NE on IL-6 production in activated peritoneal macrophages. Effects of NE on IL-6 production in the RAW264.7 macrophage cell-line also were investigated. Treatment of activated peritoneal macrophages with endotoxin, the α2-adrenergic antagonists yohimbine or RS79948 revealed that the α2-adrenergic receptor mediates a stimulatory autocrine action of catecholamines on IL-6 production. When peritoneal macrophages were treated with the β2 antagonist ICI-118,551 (ICI), there was both inhibition and stimulation of IL-6. Treatment of RAW264.7 macrophages with high and low concentrations of NE and various concentrations of ICI provided evidence that the concentration of NE determines whether the β2-adrenergic receptor mediates stimulation or inhibition of IL-6 production."],"dc:identifier":["https://doi.org/10.25772/5VA8-0E08","https://scholarscompass.vcu.edu/etd/1127"],"dc:rights":["© The Author"],"dc:subject":["peritoneal","norepinephrine","agonist","receptor","Biology","Life Sciences"],"dc:title":["Autocrine Effects of Catecholamines on Macrophage Release of Interleukin-6 (IL-6)"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T05:54:53Z"}