A Model for Studying Vasogenic Brain Edema

Convection-enhanced delivery (CED) is a proven method for targeted drug delivery to the brain that circumvents the blood-brain barrier (BBB). Little study has been conducted in understanding CED in pathological brain states. This is of importance when dealing with chemotherapeutic agent delivery to brain tumors, where vasogenic edema (VE) exists. The current study aims to characterize a model of VE suitable for studying CED.VE was produced in the right hemisphere of the rat brain using multiple infusions of hyperosmotic mannitol (0.25mL/kg/s over 30 seconds) delivered through the right internal carotid artery. Magnetic resonance imaging (MRI) revealed consistent edema formation and high water levels in the ipsilateral gray and white matter within an hour of the first infusion. Evan's Blue (EB) staining verified that VE has formed. However, apparent diffusion coefficient (ADC) and histological examination revealed also that some possible cytotoxic edema formed.This model provides a reproducible technique for generating a large area of edema for CED study. Further studies with lower doses of mannitol, while titrating to changes in ADC and values for fractional water content, may modify this model with a greater component of VE and less cerebral toxicity.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Anatomy & Neurobiology

Year dc:date.available

2006

Author dc:creator

• Shukla, Anshu

Contributors dc:contributor

• Dr. William C. Broaddus

• edema
• vasogenic edema
• hyperosmotic mannitol
• blood-brain barrier
• targeted drug delivery
• Anatomy
• Medicine and Health Sciences
• Nervous System

Statement dc:rights

• © The Author

Identifier

https://scholarscompass.vcu.edu/etd/1031

OAI identifier oai:identifier

oai:scholarscompass.vcu.edu:etd-2030