{"id":{"repo_id":"vcu","oai_identifier":"oai:scholarscompass.vcu.edu:etd-1823"},"canonical_url":"https://search.dev.ndltd.org/etd/vcu/oai:scholarscompass.vcu.edu:etd-1823","repository":{"repo_id":"vcu","name":"Virginia Commonwealth University","base_url":"https://scholarscompass.vcu.edu/do/oai/"},"display":{"title":"THE EFFECT OF SILENCING THE WILMS' TUMOR 1 GENE ON THE RADIATION SENSITIVITY OF GLIOBLASTOMA CELLS","abstract":"Glioblastomas are among the most devastating of human cancers with a median survival of only 9-12 months. This type of brain tumor is incurable, largely due its remarkable proliferative capacity and resistance to current treatments. High levels of the Wilms' Tumor 1 (WTI) gene have been identified in glioblastomas, suggesting an oncogenic function. Moreover, known WT1 target genes have been implicated in resistance to radiation. To determine the role of WT1 in radiation resistance, two glioblastoma cell lines expressing WT1 were treated with siRNAs to silence this gene. Confirmation of WT1 knockdown was achieved through real-time PCR and Western blot. After treatment with siRNA, cells were irradiated, and cell survival was assessed using a luminescent ATP assay and clonogenic survival assay. We demonstrate that treatment with WT1 siRNA increased the radiation sensitivity in both cell lines. These findings suggest that WT1 functions to protect glioblastoma cells from radiation-induced death.","abstract_html":"Glioblastomas are among the most devastating of human cancers with a median survival of only 9-12 months. This type of brain tumor is incurable, largely due its remarkable proliferative capacity and resistance to current treatments. High levels of the Wilms' Tumor 1 (WTI) gene have been identified in glioblastomas, suggesting an oncogenic function. Moreover, known WT1 target genes have been implicated in resistance to radiation. To determine the role of WT1 in radiation resistance, two glioblastoma cell lines expressing WT1 were treated with siRNAs to silence this gene. Confirmation of WT1 knockdown was achieved through real-time PCR and Western blot. After treatment with siRNA, cells were irradiated, and cell survival was assessed using a luminescent ATP assay and clonogenic survival assay. We demonstrate that treatment with WT1 siRNA increased the radiation sensitivity in both cell lines. These findings suggest that WT1 functions to protect glioblastoma cells from radiation-induced death.","abstract_has_math":false,"creators":["Chan, Dana C."],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Anatomy & Neurobiology","degree_department":null,"school":null,"contributors":["Dr. William C. Broaddus"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006-01-01T08:00:00Z","date_published":"2006-01-01T08:00:00Z","updated_at":"2026-07-24T05:54:21Z","subjects":["radiation resistance","WT1","cancer","Anatomy","Medicine and Health Sciences","Nervous System"],"languages":[],"rights":["© The Author"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarscompass.vcu.edu/etd/824"],"render_values":[{"text":"https://scholarscompass.vcu.edu/etd/824","href":"https://scholarscompass.vcu.edu/etd/824","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25772/GJC4-C658","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. William C. 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This type of brain tumor is incurable, largely due its remarkable proliferative capacity and resistance to current treatments. High levels of the Wilms' Tumor 1 (WTI) gene have been identified in glioblastomas, suggesting an oncogenic function. Moreover, known WT1 target genes have been implicated in resistance to radiation. To determine the role of WT1 in radiation resistance, two glioblastoma cell lines expressing WT1 were treated with siRNAs to silence this gene. Confirmation of WT1 knockdown was achieved through real-time PCR and Western blot. After treatment with siRNA, cells were irradiated, and cell survival was assessed using a luminescent ATP assay and clonogenic survival assay. We demonstrate that treatment with WT1 siRNA increased the radiation sensitivity in both cell lines. These findings suggest that WT1 functions to protect glioblastoma cells from radiation-induced death."]},{"key":"dc:title","label":"Title","values":["THE EFFECT OF SILENCING THE WILMS' TUMOR 1 GENE ON THE RADIATION SENSITIVITY OF GLIOBLASTOMA CELLS"]}]}],"canonical_facts":{"dc:contributor":["Dr. William C. Broaddus"],"dc:creator":["Chan, Dana C."],"dc:date.available":["2014-07-09T07:00:00Z"],"dc:description.abstract":["Glioblastomas are among the most devastating of human cancers with a median survival of only 9-12 months. This type of brain tumor is incurable, largely due its remarkable proliferative capacity and resistance to current treatments. High levels of the Wilms' Tumor 1 (WTI) gene have been identified in glioblastomas, suggesting an oncogenic function. Moreover, known WT1 target genes have been implicated in resistance to radiation. To determine the role of WT1 in radiation resistance, two glioblastoma cell lines expressing WT1 were treated with siRNAs to silence this gene. Confirmation of WT1 knockdown was achieved through real-time PCR and Western blot. After treatment with siRNA, cells were irradiated, and cell survival was assessed using a luminescent ATP assay and clonogenic survival assay. We demonstrate that treatment with WT1 siRNA increased the radiation sensitivity in both cell lines. These findings suggest that WT1 functions to protect glioblastoma cells from radiation-induced death."],"dc:identifier":["https://doi.org/10.25772/GJC4-C658","https://scholarscompass.vcu.edu/etd/824"],"dc:rights":["© The Author"],"dc:subject":["radiation resistance","WT1","cancer","Anatomy","Medicine and Health Sciences","Nervous System"],"dc:title":["THE EFFECT OF SILENCING THE WILMS' TUMOR 1 GENE ON THE RADIATION SENSITIVITY OF GLIOBLASTOMA CELLS"],"thesis:degree_discipline":["Anatomy & Neurobiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T05:54:21Z"}