{"id":{"repo_id":"vcu","oai_identifier":"oai:scholarscompass.vcu.edu:etd-1705"},"canonical_url":"https://search.dev.ndltd.org/etd/vcu/oai:scholarscompass.vcu.edu:etd-1705","repository":{"repo_id":"vcu","name":"Virginia Commonwealth University","base_url":"https://scholarscompass.vcu.edu/do/oai/"},"display":{"title":"Modulation of the Serotonin Reuptake Transporter in RAW264.7 Macrophages","abstract":"Serotonin (5-HT) plays an important role as both a neurotransmitter and animmune modulator. The serotonin reuptake transporter (SERT) clears the extracellular space of 5-HT, which decreases the effects of 5-HT on target cells. This study demonstrated that the RAW264.7 macrophage cell line expresses SERT function, measured by assays of 3H-5HT uptake. The 5-HT uptake in RAW264.7 macrophages was more than 10-fold that of peritoneal macrophages, indicating that these cells are an excellent model for studying regulation of the SERT. Activation of macrophages with lipopolysaccharide (LPS) increased SERT activity in a time- and concentration-dependent manner and Western blots indicate that the increase in activity is partially due to LPS-induced increases in total SERT protein. Both unstimulated and LPS-stimulated activity was inhibited by the specific SERT inhibitor fluoxetine (IC50= 5-8 nM) and was reduced by the anti-inflammatory cytokine interleukin-10. Changes in extracellular concentrations of interleukin-lβ and tumor necrosis factor-α did not affect SERT activity.","abstract_html":"Serotonin (5-HT) plays an important role as both a neurotransmitter and animmune modulator. The serotonin reuptake transporter (SERT) clears the extracellular space of 5-HT, which decreases the effects of 5-HT on target cells. This study demonstrated that the RAW264.7 macrophage cell line expresses SERT function, measured by assays of 3H-5HT uptake. The 5-HT uptake in RAW264.7 macrophages was more than 10-fold that of peritoneal macrophages, indicating that these cells are an excellent model for studying regulation of the SERT. Activation of macrophages with lipopolysaccharide (LPS) increased SERT activity in a time- and concentration-dependent manner and Western blots indicate that the increase in activity is partially due to LPS-induced increases in total SERT protein. Both unstimulated and LPS-stimulated activity was inhibited by the specific SERT inhibitor fluoxetine (IC50= 5-8 nM) and was reduced by the anti-inflammatory cytokine interleukin-10. Changes in extracellular concentrations of interleukin-lβ and tumor necrosis factor-α did not affect SERT activity.","abstract_has_math":false,"creators":["Malubay, Sienna Marie Arenas"],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Dr. Jennifer K. Stewart"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006-01-01T08:00:00Z","date_published":"2006-01-01T08:00:00Z","updated_at":"2026-07-24T05:54:21Z","subjects":["SERT regulation","Biology","Life Sciences"],"languages":[],"rights":["© The Author"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarscompass.vcu.edu/etd/706"],"render_values":[{"text":"https://scholarscompass.vcu.edu/etd/706","href":"https://scholarscompass.vcu.edu/etd/706","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25772/MHWD-S410","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Jennifer K. 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The serotonin reuptake transporter (SERT) clears the extracellular space of 5-HT, which decreases the effects of 5-HT on target cells. This study demonstrated that the RAW264.7 macrophage cell line expresses SERT function, measured by assays of 3H-5HT uptake. The 5-HT uptake in RAW264.7 macrophages was more than 10-fold that of peritoneal macrophages, indicating that these cells are an excellent model for studying regulation of the SERT. Activation of macrophages with lipopolysaccharide (LPS) increased SERT activity in a time- and concentration-dependent manner and Western blots indicate that the increase in activity is partially due to LPS-induced increases in total SERT protein. Both unstimulated and LPS-stimulated activity was inhibited by the specific SERT inhibitor fluoxetine (IC50= 5-8 nM) and was reduced by the anti-inflammatory cytokine interleukin-10. Changes in extracellular concentrations of interleukin-lβ and tumor necrosis factor-α did not affect SERT activity."]},{"key":"dc:title","label":"Title","values":["Modulation of the Serotonin Reuptake Transporter in RAW264.7 Macrophages"]}]}],"canonical_facts":{"dc:contributor":["Dr. Jennifer K. Stewart"],"dc:creator":["Malubay, Sienna Marie Arenas"],"dc:date.available":["2014-07-09T07:00:00Z"],"dc:description.abstract":["Serotonin (5-HT) plays an important role as both a neurotransmitter and animmune modulator. The serotonin reuptake transporter (SERT) clears the extracellular space of 5-HT, which decreases the effects of 5-HT on target cells. This study demonstrated that the RAW264.7 macrophage cell line expresses SERT function, measured by assays of 3H-5HT uptake. The 5-HT uptake in RAW264.7 macrophages was more than 10-fold that of peritoneal macrophages, indicating that these cells are an excellent model for studying regulation of the SERT. Activation of macrophages with lipopolysaccharide (LPS) increased SERT activity in a time- and concentration-dependent manner and Western blots indicate that the increase in activity is partially due to LPS-induced increases in total SERT protein. Both unstimulated and LPS-stimulated activity was inhibited by the specific SERT inhibitor fluoxetine (IC50= 5-8 nM) and was reduced by the anti-inflammatory cytokine interleukin-10. Changes in extracellular concentrations of interleukin-lβ and tumor necrosis factor-α did not affect SERT activity."],"dc:identifier":["https://doi.org/10.25772/MHWD-S410","https://scholarscompass.vcu.edu/etd/706"],"dc:rights":["© The Author"],"dc:subject":["SERT regulation","Biology","Life Sciences"],"dc:title":["Modulation of the Serotonin Reuptake Transporter in RAW264.7 Macrophages"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T05:54:21Z"}