{"id":{"repo_id":"varna","oai_identifier":"oai:repository.mu-varna.bg:nls/4114"},"canonical_url":"https://search.dev.ndltd.org/etd/varna/oai:repository.mu-varna.bg:nls/4114","repository":{"repo_id":"varna","name":"Medical University of Varna","base_url":"https://repository.mu-varna.bg/oai/request"},"display":{"title":"Biomarkers for Personalised Treatment Approach in Patients with Multiple Myeloma // Биомаркери за персонализиран подход при подбора на лечение при пациенти с миеломна болест","abstract":"The thesis aims to evaluate the role of circulating microRNAs (miRs) as diagnostic and prognostic biomarkers in multiple myeloma (MM). The cohort includes 56 untreated MM patients and 12 healthy controls, with a focus on the quantitative determination of expression levels of five selected miRs: miR-199a-5p, miR-126-5p, miR-497-5p, miR-214-3p, and miR-373-3p. The results show a significant decrease in miR-126-5p and miR-199a-5p in newly diagnosed MM patients compared to healthy controls, suggesting their potential utility as early diagnostic biomarkers. Longitudinal analysis demonstrates a significant increase in miR-126-5p levels after therapy, correlating with treatment response. In contrast, elevated expression levels of miR-214-3p and miR-497-5p are associated with unfavourable clinical outcomes, indicating their potential as prognostic indicators. Receiver operating characteristic (ROC) curve analyses reveal that miR-199a-5p has high sensitivity (87.04%) for distinguishing MM patients from controls, whereas miR-126-5p exhibits balanced diagnostic accuracy. These findings support integrating miR profiling into clinical practice to improve risk assessment and facilitate personalised therapeutic approaches in MM, ultimately aiming to enhance patient survival and quality of life.","abstract_html":"The thesis aims to evaluate the role of circulating microRNAs (miRs) as diagnostic and prognostic biomarkers in multiple myeloma (MM). The cohort includes 56 untreated MM patients and 12 healthy controls, with a focus on the quantitative determination of expression levels of five selected miRs: miR-199a-5p, miR-126-5p, miR-497-5p, miR-214-3p, and miR-373-3p. The results show a significant decrease in miR-126-5p and miR-199a-5p in newly diagnosed MM patients compared to healthy controls, suggesting their potential utility as early diagnostic biomarkers. Longitudinal analysis demonstrates a significant increase in miR-126-5p levels after therapy, correlating with treatment response. In contrast, elevated expression levels of miR-214-3p and miR-497-5p are associated with unfavourable clinical outcomes, indicating their potential as prognostic indicators. Receiver operating characteristic (ROC) curve analyses reveal that miR-199a-5p has high sensitivity (87.04%) for distinguishing MM patients from controls, whereas miR-126-5p exhibits balanced diagnostic accuracy. These findings support integrating miR profiling into clinical practice to improve risk assessment and facilitate personalised therapeutic approaches in MM, ultimately aiming to enhance patient survival and quality of life.","abstract_has_math":false,"creators":["Lukanov, Radi","Луканов, Ради"],"institution":"Medical University of Varna","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-07-17T13:36:26Z","date_published":"2025-07-17T13:36:26Z","updated_at":"2026-07-27T21:56:42Z","subjects":["multiple myeloma","biomarkers","diagnostic accuracy","Хематология / Haematology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://repository.mu-varna.bg/handle/nls/4114","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Lukanov, Radi","Луканов, Ради"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-07-17T13:36:26Z","134"]},{"key":"dc:publisher","label":"Institution","values":["Medical University of Varna"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["multiple myeloma","biomarkers","diagnostic accuracy","Хематология / Haematology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.mu-varna.bg/handle/nls/4114"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis aims to evaluate the role of circulating microRNAs (miRs) as diagnostic and prognostic biomarkers in multiple myeloma (MM). The cohort includes 56 untreated MM patients and 12 healthy controls, with a focus on the quantitative determination of expression levels of five selected miRs: miR-199a-5p, miR-126-5p, miR-497-5p, miR-214-3p, and miR-373-3p. The results show a significant decrease in miR-126-5p and miR-199a-5p in newly diagnosed MM patients compared to healthy controls, suggesting their potential utility as early diagnostic biomarkers. Longitudinal analysis demonstrates a significant increase in miR-126-5p levels after therapy, correlating with treatment response. In contrast, elevated expression levels of miR-214-3p and miR-497-5p are associated with unfavourable clinical outcomes, indicating their potential as prognostic indicators. Receiver operating characteristic (ROC) curve analyses reveal that miR-199a-5p has high sensitivity (87.04%) for distinguishing MM patients from controls, whereas miR-126-5p exhibits balanced diagnostic accuracy. These findings support integrating miR profiling into clinical practice to improve risk assessment and facilitate personalised therapeutic approaches in MM, ultimately aiming to enhance patient survival and quality of life.","Целта на настоящият научен труд е да оцени ролята на циркулиращите микроРНК (miRs) като диагностични и прогностични биомаркери при множествен миелом (ММ). Кохортата включва 56 пациенти с нелекуван ММ и 12 здрави контроли, като акцентът е поставен върху количественото определение на нивата на експресия на пет избрани miRs: miR-199a-5p, miR-126-5p, miR-497-5p, miR-214-3p и miR-373-3p. Резултатите показват значително намаление на miR-126-5p и miR-199a-5p при ново диагностицирани пациенти с ММ в сравнение със здравите контроли, което предполага тяхната потенциална полезност като биомаркери за ранна диагностика на заболяването. Лонгитудиналният анализ демонстрира, че нивата на miR-126-5p значително нарастват след терапия, корелирайки с отговора на лечението. Обратно, miR-214-3p и miR-497-5p показват повишени нива на експресия, свързани с неблагоприятни клинични резултати, което указва на техния потенциал като прогностични индикатори. Анализите на кривите на оперативната характеристика (ROC) показват, че miR-199a-5p има висока чувствителност (87.04%) за разграничаване на пациентите с ММ от контролната група, докато miR-126-5p демонстрира балансирана диагностична точност. Резултатите подкрепят интегрирането на miR профилирането в клиничната практика за подобряване на оценката на риска и улесняване на персонализираните терапевтични подходи при ММ, с крайна цел да се увеличи оцеляването на пациентите и качеството на живота им."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Biomarkers for Personalised Treatment Approach in Patients with Multiple Myeloma // Биомаркери за персонализиран подход при подбора на лечение при пациенти с миеломна болест"]}]}],"canonical_facts":{"dc:creator":["Lukanov, Radi","Луканов, Ради"],"dc:date":["2025-07-17T13:36:26Z","134"],"dc:description":["The thesis aims to evaluate the role of circulating microRNAs (miRs) as diagnostic and prognostic biomarkers in multiple myeloma (MM). The cohort includes 56 untreated MM patients and 12 healthy controls, with a focus on the quantitative determination of expression levels of five selected miRs: miR-199a-5p, miR-126-5p, miR-497-5p, miR-214-3p, and miR-373-3p. The results show a significant decrease in miR-126-5p and miR-199a-5p in newly diagnosed MM patients compared to healthy controls, suggesting their potential utility as early diagnostic biomarkers. Longitudinal analysis demonstrates a significant increase in miR-126-5p levels after therapy, correlating with treatment response. In contrast, elevated expression levels of miR-214-3p and miR-497-5p are associated with unfavourable clinical outcomes, indicating their potential as prognostic indicators. Receiver operating characteristic (ROC) curve analyses reveal that miR-199a-5p has high sensitivity (87.04%) for distinguishing MM patients from controls, whereas miR-126-5p exhibits balanced diagnostic accuracy. These findings support integrating miR profiling into clinical practice to improve risk assessment and facilitate personalised therapeutic approaches in MM, ultimately aiming to enhance patient survival and quality of life.","Целта на настоящият научен труд е да оцени ролята на циркулиращите микроРНК (miRs) като диагностични и прогностични биомаркери при множествен миелом (ММ). Кохортата включва 56 пациенти с нелекуван ММ и 12 здрави контроли, като акцентът е поставен върху количественото определение на нивата на експресия на пет избрани miRs: miR-199a-5p, miR-126-5p, miR-497-5p, miR-214-3p и miR-373-3p. Резултатите показват значително намаление на miR-126-5p и miR-199a-5p при ново диагностицирани пациенти с ММ в сравнение със здравите контроли, което предполага тяхната потенциална полезност като биомаркери за ранна диагностика на заболяването. Лонгитудиналният анализ демонстрира, че нивата на miR-126-5p значително нарастват след терапия, корелирайки с отговора на лечението. Обратно, miR-214-3p и miR-497-5p показват повишени нива на експресия, свързани с неблагоприятни клинични резултати, което указва на техния потенциал като прогностични индикатори. Анализите на кривите на оперативната характеристика (ROC) показват, че miR-199a-5p има висока чувствителност (87.04%) за разграничаване на пациентите с ММ от контролната група, докато miR-126-5p демонстрира балансирана диагностична точност. Резултатите подкрепят интегрирането на miR профилирането в клиничната практика за подобряване на оценката на риска и улесняване на персонализираните терапевтични подходи при ММ, с крайна цел да се увеличи оцеляването на пациентите и качеството на живота им."],"dc:format":["application/pdf"],"dc:identifier":["http://repository.mu-varna.bg/handle/nls/4114"],"dc:publisher":["Medical University of Varna"],"dc:subject":["multiple myeloma","biomarkers","diagnostic accuracy","Хематология / Haematology"],"dc:title":["Biomarkers for Personalised Treatment Approach in Patients with Multiple Myeloma // Биомаркери за персонализиран подход при подбора на лечение при пациенти с миеломна болест"],"dc:type":["thesis"]},"updated_at":"2026-07-27T21:56:42Z"}