{"id":{"repo_id":"varna","oai_identifier":"oai:repository.mu-varna.bg:nls/4026"},"canonical_url":"https://search.dev.ndltd.org/etd/varna/oai:repository.mu-varna.bg:nls/4026","repository":{"repo_id":"varna","name":"Medical University of Varna","base_url":"https://repository.mu-varna.bg/oai/request"},"display":{"title":"Evaluation of HB&L Automated System for Rapid Diagnosis of Urinary Tract Infections // Оценка на автоматизираната система HB&L за бърза диагностика на инфекции на уринарния тракт","abstract":"The thesis presents a prospective study of the etiological spectrum and antimicrobial resistance of the most common uropathogens in 842 outpatients in Varna over a 7-month period (October 2020 - April 2021). An automated HB&L laser nephelometry system, Alifax, was used for rapid 4-hour screening of 1600 urine samples. For the first time in Bulgaria, a parallel study of residual antibiotic activity was performed to improve the clinical interpretation of the test. Patients were recruited based on clinical symptomatology, chemical examination of urine and sediment, and a completed medical questionnaire. Antibiotic susceptibility was determined by 3 methods: standard culture method according to Bauer-Kirby, automated HB&L system and automated VITEK 2 system. An analysis of the errors obtained by the automated system was performed in a short time (3-5 hours) against the culture 24-hour reference method. Additionally, the susceptibility to three antibiotic agents used in outpatient practice was studied: fosfomycin, nitrofurantoin, and 5-nitroxoline. A high concordance of results was demonstrated. The system showed high specificity, sensitivity, and positive and negative predictive values. Based on the results, a working algorithm for the accurate and rapid diagnosis of urinary tract infections on the day of puncture was developed. Recommendations for empirical therapy in patients after a study of local resistance in the Varna region were made.","abstract_html":"The thesis presents a prospective study of the etiological spectrum and antimicrobial resistance of the most common uropathogens in 842 outpatients in Varna over a 7-month period (October 2020 - April 2021). An automated HB&L laser nephelometry system, Alifax, was used for rapid 4-hour screening of 1600 urine samples. For the first time in Bulgaria, a parallel study of residual antibiotic activity was performed to improve the clinical interpretation of the test. Patients were recruited based on clinical symptomatology, chemical examination of urine and sediment, and a completed medical questionnaire. Antibiotic susceptibility was determined by 3 methods: standard culture method according to Bauer-Kirby, automated HB&L system and automated VITEK 2 system. An analysis of the errors obtained by the automated system was performed in a short time (3-5 hours) against the culture 24-hour reference method. Additionally, the susceptibility to three antibiotic agents used in outpatient practice was studied: fosfomycin, nitrofurantoin, and 5-nitroxoline. A high concordance of results was demonstrated. The system showed high specificity, sensitivity, and positive and negative predictive values. Based on the results, a working algorithm for the accurate and rapid diagnosis of urinary tract infections on the day of puncture was developed. Recommendations for empirical therapy in patients after a study of local resistance in the Varna region were made.","abstract_has_math":false,"creators":["Snegarova-Toneva, Viktoriya","Снегарова-Тонева, Виктория"],"institution":"Medical University of Varna","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-04-29T11:53:18Z","date_published":"2025-04-29T11:53:18Z","updated_at":"2026-07-27T21:56:46Z","subjects":["antimicrobial resistance","urine","urinary tract infections","rapid diagnosis","Микробиология и вирусология / Microbiology and Virology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://repository.mu-varna.bg/handle/nls/4026","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Snegarova-Toneva, Viktoriya","Снегарова-Тонева, Виктория"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-04-29T11:53:18Z","2024"]},{"key":"dc:publisher","label":"Institution","values":["Medical University of Varna"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["antimicrobial resistance","urine","urinary tract infections","rapid diagnosis","Микробиология и вирусология / Microbiology and Virology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.mu-varna.bg/handle/nls/4026"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis presents a prospective study of the etiological spectrum and antimicrobial resistance of the most common uropathogens in 842 outpatients in Varna over a 7-month period (October 2020 - April 2021). An automated HB&L laser nephelometry system, Alifax, was used for rapid 4-hour screening of 1600 urine samples. For the first time in Bulgaria, a parallel study of residual antibiotic activity was performed to improve the clinical interpretation of the test. Patients were recruited based on clinical symptomatology, chemical examination of urine and sediment, and a completed medical questionnaire. Antibiotic susceptibility was determined by 3 methods: standard culture method according to Bauer-Kirby, automated HB&L system and automated VITEK 2 system. An analysis of the errors obtained by the automated system was performed in a short time (3-5 hours) against the culture 24-hour reference method. Additionally, the susceptibility to three antibiotic agents used in outpatient practice was studied: fosfomycin, nitrofurantoin, and 5-nitroxoline. A high concordance of results was demonstrated. The system showed high specificity, sensitivity, and positive and negative predictive values. Based on the results, a working algorithm for the accurate and rapid diagnosis of urinary tract infections on the day of puncture was developed. Recommendations for empirical therapy in patients after a study of local resistance in the Varna region were made.","Дисертационният труд се базира на проспективно проучване на етиологичния спектър и антимикробната резистентност на най-честите уропатогени в 842 амбулаторни пациенти във Варна за 7 месечен период (октомври 2020 - април 2021). За целта е използвана автоматизирана система за лазерна нефелометрия HB&L, Alifax за бърз 4 часов скрининг на 1600 проби урина. За първи път в България е осъществено паралелно изследване на остатъчна антибиотична активност, за да се подобри клиничната интерпретация на теста. Пациентите са набирани на база клинична симптоматика, химично изследване на урина и седимент, попълнена медицинска анкета. Антибиотичната чувствителност е определена по 3 методики: стандартен културелен метод по Bauer – Kirby, автоматизирана система HB&L и автоматизирана система VITEK 2. Осъществен е анализ на получените грешки на автоматизираната система за кратко време (3-5 часа) спрямо културелния 24 часов референтен метод. Допълнително е проучена чувствителността към три антибиотични препарата, използвани в амбулаторната практика: fosfomycin, nitrofurantoin, 5-nitroxoline. Доказва се висока конкордантност на резултатите. Системата показва висока специфичност, чувствителност, положителна и отрицателна предиктивна стойност. Базирайки се на получените резултати е разработен работен алгоритъм за точна и бърза диагностика на уроинфекциите в деня на пободването. Изготвени са препоръки за емпирична терапия при пациенти след проучване на локалната резистентност във Варненски регион."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Evaluation of HB&L Automated System for Rapid Diagnosis of Urinary Tract Infections // Оценка на автоматизираната система HB&L за бърза диагностика на инфекции на уринарния тракт"]}]}],"canonical_facts":{"dc:creator":["Snegarova-Toneva, Viktoriya","Снегарова-Тонева, Виктория"],"dc:date":["2025-04-29T11:53:18Z","2024"],"dc:description":["The thesis presents a prospective study of the etiological spectrum and antimicrobial resistance of the most common uropathogens in 842 outpatients in Varna over a 7-month period (October 2020 - April 2021). An automated HB&L laser nephelometry system, Alifax, was used for rapid 4-hour screening of 1600 urine samples. For the first time in Bulgaria, a parallel study of residual antibiotic activity was performed to improve the clinical interpretation of the test. Patients were recruited based on clinical symptomatology, chemical examination of urine and sediment, and a completed medical questionnaire. Antibiotic susceptibility was determined by 3 methods: standard culture method according to Bauer-Kirby, automated HB&L system and automated VITEK 2 system. An analysis of the errors obtained by the automated system was performed in a short time (3-5 hours) against the culture 24-hour reference method. Additionally, the susceptibility to three antibiotic agents used in outpatient practice was studied: fosfomycin, nitrofurantoin, and 5-nitroxoline. A high concordance of results was demonstrated. The system showed high specificity, sensitivity, and positive and negative predictive values. Based on the results, a working algorithm for the accurate and rapid diagnosis of urinary tract infections on the day of puncture was developed. Recommendations for empirical therapy in patients after a study of local resistance in the Varna region were made.","Дисертационният труд се базира на проспективно проучване на етиологичния спектър и антимикробната резистентност на най-честите уропатогени в 842 амбулаторни пациенти във Варна за 7 месечен период (октомври 2020 - април 2021). За целта е използвана автоматизирана система за лазерна нефелометрия HB&L, Alifax за бърз 4 часов скрининг на 1600 проби урина. За първи път в България е осъществено паралелно изследване на остатъчна антибиотична активност, за да се подобри клиничната интерпретация на теста. Пациентите са набирани на база клинична симптоматика, химично изследване на урина и седимент, попълнена медицинска анкета. Антибиотичната чувствителност е определена по 3 методики: стандартен културелен метод по Bauer – Kirby, автоматизирана система HB&L и автоматизирана система VITEK 2. Осъществен е анализ на получените грешки на автоматизираната система за кратко време (3-5 часа) спрямо културелния 24 часов референтен метод. Допълнително е проучена чувствителността към три антибиотични препарата, използвани в амбулаторната практика: fosfomycin, nitrofurantoin, 5-nitroxoline. Доказва се висока конкордантност на резултатите. Системата показва висока специфичност, чувствителност, положителна и отрицателна предиктивна стойност. Базирайки се на получените резултати е разработен работен алгоритъм за точна и бърза диагностика на уроинфекциите в деня на пободването. Изготвени са препоръки за емпирична терапия при пациенти след проучване на локалната резистентност във Варненски регион."],"dc:format":["application/pdf"],"dc:identifier":["http://repository.mu-varna.bg/handle/nls/4026"],"dc:publisher":["Medical University of Varna"],"dc:subject":["antimicrobial resistance","urine","urinary tract infections","rapid diagnosis","Микробиология и вирусология / Microbiology and Virology"],"dc:title":["Evaluation of HB&L Automated System for Rapid Diagnosis of Urinary Tract Infections // Оценка на автоматизираната система HB&L за бърза диагностика на инфекции на уринарния тракт"],"dc:type":["thesis"]},"updated_at":"2026-07-27T21:56:46Z"}