{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/37522"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/37522","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"SUFU in SHH signalling mediated myogenesis","abstract":"Myogenesis is defined as the formation of skeletal muscle tissue during embryonic development and involves a multitude of cellular signalling pathways. Among these include the Sonic hedgehog (Shh) signalling pathway which must be deactivated for differentiation into muscle cells to occur. However, less is known regarding the pathways operation during cell differentiation and whether Suppressor of Fused (SUFU), the protein inhibitor of Shh signalling, plays a role. To address this, mouse C2C12 myoblast cells were utilized as a model and differentiated into muscle cells to identify the presence of SUFU during this time. Experiments in qRT-PCR show a decrease in Shh responsive gene transcripts after induced differentiation thus confirming the deactivation of the pathway during this time. Alongside this, immunoblotting results show the absence of SUFU during proliferation of the cells and its presence throughout differentiation. These results postulate a role for SUFU as an inhibitor during Shh mediated myogenesis.","abstract_html":"Myogenesis is defined as the formation of skeletal muscle tissue during embryonic development and involves a multitude of cellular signalling pathways. Among these include the Sonic hedgehog (Shh) signalling pathway which must be deactivated for differentiation into muscle cells to occur. However, less is known regarding the pathways operation during cell differentiation and whether Suppressor of Fused (SUFU), the protein inhibitor of Shh signalling, plays a role. To address this, mouse C2C12 myoblast cells were utilized as a model and differentiated into muscle cells to identify the presence of SUFU during this time. Experiments in qRT-PCR show a decrease in Shh responsive gene transcripts after induced differentiation thus confirming the deactivation of the pathway during this time. Alongside this, immunoblotting results show the absence of SUFU during proliferation of the cells and its presence throughout differentiation. These results postulate a role for SUFU as an inhibitor during Shh mediated myogenesis.","abstract_has_math":false,"creators":["Abdullah, Suleyman"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Kelly, Gregory"],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12-11","date_published":"2023-12-11","updated_at":"2026-07-27T21:56:11Z","subjects":["Muscle","Myogenesis","Development","Differentiation","Sonic hedgehog","Suppressor of Fused"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/37522","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Kelly, Gregory"]},{"key":"dc:creator","label":"Author","values":["Abdullah, Suleyman"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T21:37:40Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T21:37:40Z"]},{"key":"dc:date.issued","label":"Date","values":["2023-12-11"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Muscle","Myogenesis","Development","Differentiation","Sonic hedgehog","Suppressor of Fused"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/37522"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Collaborative Specialization: Developmental Biology","The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Myogenesis is defined as the formation of skeletal muscle tissue during embryonic development and involves a multitude of cellular signalling pathways. Among these include the Sonic hedgehog (Shh) signalling pathway which must be deactivated for differentiation into muscle cells to occur. However, less is known regarding the pathways operation during cell differentiation and whether Suppressor of Fused (SUFU), the protein inhibitor of Shh signalling, plays a role. To address this, mouse C2C12 myoblast cells were utilized as a model and differentiated into muscle cells to identify the presence of SUFU during this time. Experiments in qRT-PCR show a decrease in Shh responsive gene transcripts after induced differentiation thus confirming the deactivation of the pathway during this time. Alongside this, immunoblotting results show the absence of SUFU during proliferation of the cells and its presence throughout differentiation. These results postulate a role for SUFU as an inhibitor during Shh mediated myogenesis."]},{"key":"dc:title","label":"Title","values":["SUFU in SHH signalling mediated myogenesis"]}]}],"canonical_facts":{"dc:contributor.advisor":["Kelly, Gregory"],"dc:creator":["Abdullah, Suleyman"],"dc:date.accessioned":["2025-07-10T21:37:40Z"],"dc:date.available":["2025-07-10T21:37:40Z"],"dc:date.issued":["2023-12-11"],"dc:description":["Collaborative Specialization: Developmental Biology","The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Myogenesis is defined as the formation of skeletal muscle tissue during embryonic development and involves a multitude of cellular signalling pathways. Among these include the Sonic hedgehog (Shh) signalling pathway which must be deactivated for differentiation into muscle cells to occur. However, less is known regarding the pathways operation during cell differentiation and whether Suppressor of Fused (SUFU), the protein inhibitor of Shh signalling, plays a role. To address this, mouse C2C12 myoblast cells were utilized as a model and differentiated into muscle cells to identify the presence of SUFU during this time. Experiments in qRT-PCR show a decrease in Shh responsive gene transcripts after induced differentiation thus confirming the deactivation of the pathway during this time. Alongside this, immunoblotting results show the absence of SUFU during proliferation of the cells and its presence throughout differentiation. These results postulate a role for SUFU as an inhibitor during Shh mediated myogenesis."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/37522"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Muscle","Myogenesis","Development","Differentiation","Sonic hedgehog","Suppressor of Fused"],"dc:title":["SUFU in SHH signalling mediated myogenesis"],"dc:type":["thesis"],"thesis:degree_discipline":["Biology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:11Z"}