{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/36491"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/36491","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"The Interaction of the Human Adenovirus E1A Protein with the Human DREF Transcription Factor","abstract":"The human adenovirus (HAdV) E1A protein is the first protein produced post-HAdV infection, and serves two main functions. The first is to modulate host and viral transcription. The second is to induce host cell cycle progression to S phase, to promote an optimal environment for viral replication. E1A performs its functions by binding and manipulating over 50 cellular factors. Interestingly, I found that E1A is capable of interacting with the poorly characterized human DNA replication-related element-binding factor (hDREF). hDREF is a transcription factor associated with the expression of several genes related to the cell cycle. I hypothesized that the interaction between E1A and hDREF would contribute to adenovirus induced transcriptional modulation and viral replication in HAdV-5 infected host cells. Utilizing co-immunoprecipitation experiments, I discovered that E1A can bind hDREF through residues 15-26. Using quantitative real time polymerase chain reaction (RT-PCR), I found that hDREF also increases expression of HAdV-5 E3 and E4 genes, which are trans-activated by E1A. Finally, hDREF expression increases HAdV-5 replication. Further studies will reveal whether or not the E1A-hDREF interaction is specifically responsible for these observed results.","abstract_html":"The human adenovirus (HAdV) E1A protein is the first protein produced post-HAdV infection, and serves two main functions. The first is to modulate host and viral transcription. The second is to induce host cell cycle progression to S phase, to promote an optimal environment for viral replication. E1A performs its functions by binding and manipulating over 50 cellular factors. Interestingly, I found that E1A is capable of interacting with the poorly characterized human DNA replication-related element-binding factor (hDREF). hDREF is a transcription factor associated with the expression of several genes related to the cell cycle. I hypothesized that the interaction between E1A and hDREF would contribute to adenovirus induced transcriptional modulation and viral replication in HAdV-5 infected host cells. Utilizing co-immunoprecipitation experiments, I discovered that E1A can bind hDREF through residues 15-26. Using quantitative real time polymerase chain reaction (RT-PCR), I found that hDREF also increases expression of HAdV-5 E3 and E4 genes, which are trans-activated by E1A. Finally, hDREF expression increases HAdV-5 replication. Further studies will reveal whether or not the E1A-hDREF interaction is specifically responsible for these observed results.","abstract_has_math":false,"creators":["James, Kris M"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Microbiology and Immunology","degree_department":null,"school":null,"contributors":[],"advisors":["Joe Mymryk"],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-08-22","date_published":"2013-08-22","updated_at":"2026-07-27T21:56:07Z","subjects":["human adenovirus","HAdV","E1A","hDREF","co-immoprecipitation"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/36491","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Joe Mymryk"]},{"key":"dc:creator","label":"Author","values":["James, Kris M"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T21:22:20Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T21:22:20Z"]},{"key":"dc:date.issued","label":"Date","values":["2013-08-22"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology and Immunology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["human adenovirus","HAdV","E1A","hDREF","co-immoprecipitation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/36491"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["The human adenovirus (HAdV) E1A protein is the first protein produced post-HAdV infection, and serves two main functions. The first is to modulate host and viral transcription. The second is to induce host cell cycle progression to S phase, to promote an optimal environment for viral replication. E1A performs its functions by binding and manipulating over 50 cellular factors. Interestingly, I found that E1A is capable of interacting with the poorly characterized human DNA replication-related element-binding factor (hDREF). hDREF is a transcription factor associated with the expression of several genes related to the cell cycle. I hypothesized that the interaction between E1A and hDREF would contribute to adenovirus induced transcriptional modulation and viral replication in HAdV-5 infected host cells. Utilizing co-immunoprecipitation experiments, I discovered that E1A can bind hDREF through residues 15-26. Using quantitative real time polymerase chain reaction (RT-PCR), I found that hDREF also increases expression of HAdV-5 E3 and E4 genes, which are trans-activated by E1A. Finally, hDREF expression increases HAdV-5 replication. Further studies will reveal whether or not the E1A-hDREF interaction is specifically responsible for these observed results."]},{"key":"dc:title","label":"Title","values":["The Interaction of the Human Adenovirus E1A Protein with the Human DREF Transcription Factor"]}]}],"canonical_facts":{"dc:contributor.advisor":["Joe Mymryk"],"dc:creator":["James, Kris M"],"dc:date.accessioned":["2025-07-10T21:22:20Z"],"dc:date.available":["2025-07-10T21:22:20Z"],"dc:date.issued":["2013-08-22"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["The human adenovirus (HAdV) E1A protein is the first protein produced post-HAdV infection, and serves two main functions. The first is to modulate host and viral transcription. The second is to induce host cell cycle progression to S phase, to promote an optimal environment for viral replication. E1A performs its functions by binding and manipulating over 50 cellular factors. Interestingly, I found that E1A is capable of interacting with the poorly characterized human DNA replication-related element-binding factor (hDREF). hDREF is a transcription factor associated with the expression of several genes related to the cell cycle. I hypothesized that the interaction between E1A and hDREF would contribute to adenovirus induced transcriptional modulation and viral replication in HAdV-5 infected host cells. Utilizing co-immunoprecipitation experiments, I discovered that E1A can bind hDREF through residues 15-26. Using quantitative real time polymerase chain reaction (RT-PCR), I found that hDREF also increases expression of HAdV-5 E3 and E4 genes, which are trans-activated by E1A. Finally, hDREF expression increases HAdV-5 replication. Further studies will reveal whether or not the E1A-hDREF interaction is specifically responsible for these observed results."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/36491"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["human adenovirus","HAdV","E1A","hDREF","co-immoprecipitation"],"dc:title":["The Interaction of the Human Adenovirus E1A Protein with the Human DREF Transcription Factor"],"dc:type":["thesis"],"thesis:degree_discipline":["Microbiology and Immunology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:07Z"}