HcpE, a potential immuno-modulatory protein from Helicobacter pylori that is dependent on the Disulfide bond protein DsbHP
H. pylori is a human gastric pathogen that colonizes ~ 50% of the world’s population. It can cause gastritis, gastric or duodenal ulcers and also gastric cancer. H. pylori produces Helicobacter cysteine rich protein HcpE, a secreted protein which may play a role in virulence. In this study we show that HcpE is secreted in the culture supernatant both as a soluble protein and in association with outer membrane vesicles, and may play a role in the modulation of H. pylori inflammatory responses. We identified that DsbHP is necessary for HcpE production and secretion in H. pylori, and demonstrated DsbHP has DiSulfide Bond (Dsb) forming activity on reduced lysozyme. Furthermore, we demonstrated that DsbHP has a DsbA-type of activity when expressed in E. coli, despite its similarity with DsbG, and that DsbHP is involved in maintaining redox homeostasis in H. pylori.
Helicobacter pylori, Disulfide bonds, Dsb proteins, Helicobacter Cysteine-rich proteins, Protein secretion
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