{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/35488"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/35488","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Modulation of the Cardiomyocyte Hypertrophic Responses to Endothelin-1 by Adipocytes","abstract":"Leptin and adiponectin are the adipokines that are shown to exert pro-hypertrophic and anti-hypertrophic effects respectively, in cardiomyocytes. We sought to determine the direct interaction between the adipocytes and cardiomyocytes during endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy and determine the role of leptin and adiponectin. The adipose tissue conditioned medium (ACM) inhibited the ET-1-induced hypertrophy in a concentration-dependent manner and this anti-hypertrophic effect was more potent or reversed in the presence of leptin receptor antagonist (LRA; 0.1 nM) or adiponectin receptor-1 antibody (ARA; 100 ng/mL), respectively. ACM from heart failure rats induced by coronary artery ligation or obese rats did not mitigate the ET-1 induced hypertrophy in cardiomyocytes. These studies demonstrate that the net anti-hypertrophic activity of ACM in ET-1-induced cardiac hypertrophy is likely mediated by adiponectin due activation of AMPK and determined by the leptin to adiponectin ratio. Heart failure and obese-induced pathology changes the characteristics of adipocytes although the underlying mechanisms for this effect are not completely known.","abstract_html":"Leptin and adiponectin are the adipokines that are shown to exert pro-hypertrophic and anti-hypertrophic effects respectively, in cardiomyocytes. We sought to determine the direct interaction between the adipocytes and cardiomyocytes during endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy and determine the role of leptin and adiponectin. The adipose tissue conditioned medium (ACM) inhibited the ET-1-induced hypertrophy in a concentration-dependent manner and this anti-hypertrophic effect was more potent or reversed in the presence of leptin receptor antagonist (LRA; 0.1 nM) or adiponectin receptor-1 antibody (ARA; 100 ng/mL), respectively. ACM from heart failure rats induced by coronary artery ligation or obese rats did not mitigate the ET-1 induced hypertrophy in cardiomyocytes. These studies demonstrate that the net anti-hypertrophic activity of ACM in ET-1-induced cardiac hypertrophy is likely mediated by adiponectin due activation of AMPK and determined by the leptin to adiponectin ratio. Heart failure and obese-induced pathology changes the characteristics of adipocytes although the underlying mechanisms for this effect are not completely known.","abstract_has_math":false,"creators":["Bairwa, Suresh Chandra"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Physiology and Pharmacology","degree_department":null,"school":null,"contributors":[],"advisors":["Morris Karmazyn"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-15","date_published":"2014-12-15","updated_at":"2026-07-27T21:56:09Z","subjects":["Cardiac hypertrophy","heart failure","cardiomyocytes","leptin","adiponectin","ACM","adipocytes","ET-1","obesity"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/35488","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Morris Karmazyn"]},{"key":"dc:creator","label":"Author","values":["Bairwa, Suresh Chandra"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T20:38:15Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T20:38:15Z"]},{"key":"dc:date.issued","label":"Date","values":["2014-12-15"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology and Pharmacology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cardiac hypertrophy","heart failure","cardiomyocytes","leptin","adiponectin","ACM","adipocytes","ET-1","obesity"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/35488"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Leptin and adiponectin are the adipokines that are shown to exert pro-hypertrophic and anti-hypertrophic effects respectively, in cardiomyocytes. We sought to determine the direct interaction between the adipocytes and cardiomyocytes during endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy and determine the role of leptin and adiponectin. The adipose tissue conditioned medium (ACM) inhibited the ET-1-induced hypertrophy in a concentration-dependent manner and this anti-hypertrophic effect was more potent or reversed in the presence of leptin receptor antagonist (LRA; 0.1 nM) or adiponectin receptor-1 antibody (ARA; 100 ng/mL), respectively. ACM from heart failure rats induced by coronary artery ligation or obese rats did not mitigate the ET-1 induced hypertrophy in cardiomyocytes. These studies demonstrate that the net anti-hypertrophic activity of ACM in ET-1-induced cardiac hypertrophy is likely mediated by adiponectin due activation of AMPK and determined by the leptin to adiponectin ratio. Heart failure and obese-induced pathology changes the characteristics of adipocytes although the underlying mechanisms for this effect are not completely known."]},{"key":"dc:title","label":"Title","values":["Modulation of the Cardiomyocyte Hypertrophic Responses to Endothelin-1 by Adipocytes"]}]}],"canonical_facts":{"dc:contributor.advisor":["Morris Karmazyn"],"dc:creator":["Bairwa, Suresh Chandra"],"dc:date.accessioned":["2025-07-10T20:38:15Z"],"dc:date.available":["2025-07-10T20:38:15Z"],"dc:date.issued":["2014-12-15"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Leptin and adiponectin are the adipokines that are shown to exert pro-hypertrophic and anti-hypertrophic effects respectively, in cardiomyocytes. We sought to determine the direct interaction between the adipocytes and cardiomyocytes during endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy and determine the role of leptin and adiponectin. The adipose tissue conditioned medium (ACM) inhibited the ET-1-induced hypertrophy in a concentration-dependent manner and this anti-hypertrophic effect was more potent or reversed in the presence of leptin receptor antagonist (LRA; 0.1 nM) or adiponectin receptor-1 antibody (ARA; 100 ng/mL), respectively. ACM from heart failure rats induced by coronary artery ligation or obese rats did not mitigate the ET-1 induced hypertrophy in cardiomyocytes. These studies demonstrate that the net anti-hypertrophic activity of ACM in ET-1-induced cardiac hypertrophy is likely mediated by adiponectin due activation of AMPK and determined by the leptin to adiponectin ratio. Heart failure and obese-induced pathology changes the characteristics of adipocytes although the underlying mechanisms for this effect are not completely known."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/35488"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Cardiac hypertrophy","heart failure","cardiomyocytes","leptin","adiponectin","ACM","adipocytes","ET-1","obesity"],"dc:title":["Modulation of the Cardiomyocyte Hypertrophic Responses to Endothelin-1 by Adipocytes"],"dc:type":["thesis"],"thesis:degree_discipline":["Physiology and Pharmacology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:09Z"}