{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/35159"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/35159","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"The Effects of RHAMM on Cell Division, Cell Motility, and Tumorigenesis","abstract":"Overexpression of a RHAMM isoform (RHAMMΔ163) transforms fibroblasts but the mechanisms underlying this oncogenic function are not well understood. RHAMMΔ163 binds to the mitotic spindle and centrosomes via a C-terminal leucine zipper; these interactions are predicted to regulate genomic stability and cell polarity and proposed to account for the oncogenic function of RHAMMΔ163. We hypothesized that RHAMM leucine zipper maintains mitotic spindle integrity and impacts directional cell migration through its interactions with microtubule and centrosome structures. The consequences of a mutated leucine zipper on cell division, cell motility, and tumorigenesis were assessed. Although mutant RHAMMΔ163 promoted polycentrosomy, it did not alter cell cycle progression and did not strongly affect proliferation or tumorigenesis. However, loss of the leucine zipper function blocked directional movement of fibroblasts without affecting rate of motility. These results suggest that the RHAMM leucine zipper selectively regulates directed migration, which is a centrosome function that contributes to tumorigenesis.","abstract_html":"Overexpression of a RHAMM isoform (RHAMMΔ163) transforms fibroblasts but the mechanisms underlying this oncogenic function are not well understood. RHAMMΔ163 binds to the mitotic spindle and centrosomes via a C-terminal leucine zipper; these interactions are predicted to regulate genomic stability and cell polarity and proposed to account for the oncogenic function of RHAMMΔ163. We hypothesized that RHAMM leucine zipper maintains mitotic spindle integrity and impacts directional cell migration through its interactions with microtubule and centrosome structures. The consequences of a mutated leucine zipper on cell division, cell motility, and tumorigenesis were assessed. Although mutant RHAMMΔ163 promoted polycentrosomy, it did not alter cell cycle progression and did not strongly affect proliferation or tumorigenesis. However, loss of the leucine zipper function blocked directional movement of fibroblasts without affecting rate of motility. These results suggest that the RHAMM leucine zipper selectively regulates directed migration, which is a centrosome function that contributes to tumorigenesis.","abstract_has_math":false,"creators":["Elhayek, Sallie"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":[],"advisors":["Eva Turley"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-08-26","date_published":"2014-08-26","updated_at":"2026-07-27T21:55:56Z","subjects":["RHAMM","leucine zipper","mitosis","microtubules","centrosomes","cell migration"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/35159","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Eva Turley"]},{"key":"dc:creator","label":"Author","values":["Elhayek, Sallie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T20:35:28Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T20:35:28Z"]},{"key":"dc:date.issued","label":"Date","values":["2014-08-26"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["RHAMM","leucine zipper","mitosis","microtubules","centrosomes","cell migration"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/35159"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Overexpression of a RHAMM isoform (RHAMMΔ163) transforms fibroblasts but the mechanisms underlying this oncogenic function are not well understood. RHAMMΔ163 binds to the mitotic spindle and centrosomes via a C-terminal leucine zipper; these interactions are predicted to regulate genomic stability and cell polarity and proposed to account for the oncogenic function of RHAMMΔ163. We hypothesized that RHAMM leucine zipper maintains mitotic spindle integrity and impacts directional cell migration through its interactions with microtubule and centrosome structures. The consequences of a mutated leucine zipper on cell division, cell motility, and tumorigenesis were assessed. Although mutant RHAMMΔ163 promoted polycentrosomy, it did not alter cell cycle progression and did not strongly affect proliferation or tumorigenesis. However, loss of the leucine zipper function blocked directional movement of fibroblasts without affecting rate of motility. These results suggest that the RHAMM leucine zipper selectively regulates directed migration, which is a centrosome function that contributes to tumorigenesis."]},{"key":"dc:title","label":"Title","values":["The Effects of RHAMM on Cell Division, Cell Motility, and Tumorigenesis"]}]}],"canonical_facts":{"dc:contributor.advisor":["Eva Turley"],"dc:creator":["Elhayek, Sallie"],"dc:date.accessioned":["2025-07-10T20:35:28Z"],"dc:date.available":["2025-07-10T20:35:28Z"],"dc:date.issued":["2014-08-26"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Overexpression of a RHAMM isoform (RHAMMΔ163) transforms fibroblasts but the mechanisms underlying this oncogenic function are not well understood. RHAMMΔ163 binds to the mitotic spindle and centrosomes via a C-terminal leucine zipper; these interactions are predicted to regulate genomic stability and cell polarity and proposed to account for the oncogenic function of RHAMMΔ163. We hypothesized that RHAMM leucine zipper maintains mitotic spindle integrity and impacts directional cell migration through its interactions with microtubule and centrosome structures. The consequences of a mutated leucine zipper on cell division, cell motility, and tumorigenesis were assessed. Although mutant RHAMMΔ163 promoted polycentrosomy, it did not alter cell cycle progression and did not strongly affect proliferation or tumorigenesis. However, loss of the leucine zipper function blocked directional movement of fibroblasts without affecting rate of motility. These results suggest that the RHAMM leucine zipper selectively regulates directed migration, which is a centrosome function that contributes to tumorigenesis."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/35159"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["RHAMM","leucine zipper","mitosis","microtubules","centrosomes","cell migration"],"dc:title":["The Effects of RHAMM on Cell Division, Cell Motility, and Tumorigenesis"],"dc:type":["thesis"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:55:56Z"}