{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/33960"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/33960","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Cardiovascular Characterization of Cx40/Panx1 Single and Double Knockout Mice","abstract":"Connexins (Cxs) and pannexins (Panxs) are protein families that form large-pore channels which exist at the plasma membrane for both intracellular and extracellular signaling. Given their potential for overlapping cellular signaling functions we proposed that mice lacking both a connexin and a pannexin would have a severe phenotype. To investigate this possibility we crossed Panx1 null mice with Cx40 knockout mice and characterized the first global connexin/pannexin double knockout mouse. Intriguingly, the combined ablation of both Cx40 and Panx1 caused decreased prenatal and newborn survival, but did not affect the fertility or lifespan of surviving mice. Cx40-/- and Cx40-/-Panx1-/- mice had cardiac hypertrophy, and furthermore, combined channel ablation led to increasing severe hypertension and decreased endothelium dependent vasodilation in Cx40-/-Panx1-/- mice. Overall, these studies suggest that even though Panx1 and Cx40 act via differential mechanisms, they have a co-regulatory role in certain physiological processes such as vascular response.","abstract_html":"Connexins (Cxs) and pannexins (Panxs) are protein families that form large-pore channels which exist at the plasma membrane for both intracellular and extracellular signaling. Given their potential for overlapping cellular signaling functions we proposed that mice lacking both a connexin and a pannexin would have a severe phenotype. To investigate this possibility we crossed Panx1 null mice with Cx40 knockout mice and characterized the first global connexin/pannexin double knockout mouse. Intriguingly, the combined ablation of both Cx40 and Panx1 caused decreased prenatal and newborn survival, but did not affect the fertility or lifespan of surviving mice. Cx40-/- and Cx40-/-Panx1-/- mice had cardiac hypertrophy, and furthermore, combined channel ablation led to increasing severe hypertension and decreased endothelium dependent vasodilation in Cx40-/-Panx1-/- mice. Overall, these studies suggest that even though Panx1 and Cx40 act via differential mechanisms, they have a co-regulatory role in certain physiological processes such as vascular response.","abstract_has_math":false,"creators":["Jelen, Meghan L"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Anatomy and Cell Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Dale Laird"],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-07-25","date_published":"2016-07-25","updated_at":"2026-07-27T21:55:59Z","subjects":["Gap Junctions","Connexins","Pannxins","Cardiovascular System","Cx40","Panx1"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/33960","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Dale Laird"]},{"key":"dc:creator","label":"Author","values":["Jelen, Meghan L"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T19:56:56Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T19:56:56Z"]},{"key":"dc:date.issued","label":"Date","values":["2016-07-25"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Anatomy and Cell Biology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Gap Junctions","Connexins","Pannxins","Cardiovascular System","Cx40","Panx1"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/33960"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Connexins (Cxs) and pannexins (Panxs) are protein families that form large-pore channels which exist at the plasma membrane for both intracellular and extracellular signaling. Given their potential for overlapping cellular signaling functions we proposed that mice lacking both a connexin and a pannexin would have a severe phenotype. To investigate this possibility we crossed Panx1 null mice with Cx40 knockout mice and characterized the first global connexin/pannexin double knockout mouse. Intriguingly, the combined ablation of both Cx40 and Panx1 caused decreased prenatal and newborn survival, but did not affect the fertility or lifespan of surviving mice. Cx40-/- and Cx40-/-Panx1-/- mice had cardiac hypertrophy, and furthermore, combined channel ablation led to increasing severe hypertension and decreased endothelium dependent vasodilation in Cx40-/-Panx1-/- mice. Overall, these studies suggest that even though Panx1 and Cx40 act via differential mechanisms, they have a co-regulatory role in certain physiological processes such as vascular response."]},{"key":"dc:title","label":"Title","values":["Cardiovascular Characterization of Cx40/Panx1 Single and Double Knockout Mice"]}]}],"canonical_facts":{"dc:contributor.advisor":["Dale Laird"],"dc:creator":["Jelen, Meghan L"],"dc:date.accessioned":["2025-07-10T19:56:56Z"],"dc:date.available":["2025-07-10T19:56:56Z"],"dc:date.issued":["2016-07-25"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Connexins (Cxs) and pannexins (Panxs) are protein families that form large-pore channels which exist at the plasma membrane for both intracellular and extracellular signaling. Given their potential for overlapping cellular signaling functions we proposed that mice lacking both a connexin and a pannexin would have a severe phenotype. To investigate this possibility we crossed Panx1 null mice with Cx40 knockout mice and characterized the first global connexin/pannexin double knockout mouse. Intriguingly, the combined ablation of both Cx40 and Panx1 caused decreased prenatal and newborn survival, but did not affect the fertility or lifespan of surviving mice. Cx40-/- and Cx40-/-Panx1-/- mice had cardiac hypertrophy, and furthermore, combined channel ablation led to increasing severe hypertension and decreased endothelium dependent vasodilation in Cx40-/-Panx1-/- mice. Overall, these studies suggest that even though Panx1 and Cx40 act via differential mechanisms, they have a co-regulatory role in certain physiological processes such as vascular response."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/33960"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Gap Junctions","Connexins","Pannxins","Cardiovascular System","Cx40","Panx1"],"dc:title":["Cardiovascular Characterization of Cx40/Panx1 Single and Double Knockout Mice"],"dc:type":["thesis"],"thesis:degree_discipline":["Anatomy and Cell Biology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:55:59Z"}