{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/33671"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/33671","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"A Genetic Test of a Model for Two Activities of Fushi Tarazu Protein in Drosophila Melanogaster","abstract":"The important pair-rule segmentation gene fushi tarazu (ftz) encodes a homeodomain (HD)-containing protein involved in the establishment of even-numbered parasegments during embryonic development. The D. melanogaster ftz is a derived homeotic selector (Hox) gene which lost its homeotic function during the evolution of arthropods. Genetic analyses have shown that FTZ has two distinct activities required during development: HD-dependent and HD-independent FTZ activities. The aim of this study was to test the interaction of the two FTZ activities proposed by Hyduk and Percival-Smith (1996), by generating site-specific mutant ftz alleles for intragenic complementation. CRISPR-mediated homology directed repair (HDR) was used to introduce engineered ftz alleles into the ftz locus. Subsequently, four ftz engineered alleles were constructed in vectors for reintroduction by Recombinase-mediated cassette exchange (RMCE). Despite using multiple approaches no CRISPR mediated HDR events were detected, and therefore, the model could not be tested.","abstract_html":"The important pair-rule segmentation gene fushi tarazu (ftz) encodes a homeodomain (HD)-containing protein involved in the establishment of even-numbered parasegments during embryonic development. The D. melanogaster ftz is a derived homeotic selector (Hox) gene which lost its homeotic function during the evolution of arthropods. Genetic analyses have shown that FTZ has two distinct activities required during development: HD-dependent and HD-independent FTZ activities. The aim of this study was to test the interaction of the two FTZ activities proposed by Hyduk and Percival-Smith (1996), by generating site-specific mutant ftz alleles for intragenic complementation. CRISPR-mediated homology directed repair (HDR) was used to introduce engineered ftz alleles into the ftz locus. Subsequently, four ftz engineered alleles were constructed in vectors for reintroduction by Recombinase-mediated cassette exchange (RMCE). Despite using multiple approaches no CRISPR mediated HDR events were detected, and therefore, the model could not be tested.","abstract_has_math":false,"creators":["Briek, Alaa"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Percival-Smith, Anthony"],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-04-19","date_published":"2016-04-19","updated_at":"2026-07-27T21:56:14Z","subjects":["Drosophila melanogaster","pair rule genes","fushi tarazu","homeodomain","segmentation","CRISPR/Cas9."],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/33671","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Percival-Smith, Anthony"]},{"key":"dc:creator","label":"Author","values":["Briek, Alaa"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T19:54:38Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T19:54:38Z"]},{"key":"dc:date.issued","label":"Date","values":["2016-04-19"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Drosophila melanogaster","pair rule genes","fushi tarazu","homeodomain","segmentation","CRISPR/Cas9."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/33671"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["The important pair-rule segmentation gene fushi tarazu (ftz) encodes a homeodomain (HD)-containing protein involved in the establishment of even-numbered parasegments during embryonic development. The D. melanogaster ftz is a derived homeotic selector (Hox) gene which lost its homeotic function during the evolution of arthropods. Genetic analyses have shown that FTZ has two distinct activities required during development: HD-dependent and HD-independent FTZ activities. The aim of this study was to test the interaction of the two FTZ activities proposed by Hyduk and Percival-Smith (1996), by generating site-specific mutant ftz alleles for intragenic complementation. CRISPR-mediated homology directed repair (HDR) was used to introduce engineered ftz alleles into the ftz locus. Subsequently, four ftz engineered alleles were constructed in vectors for reintroduction by Recombinase-mediated cassette exchange (RMCE). Despite using multiple approaches no CRISPR mediated HDR events were detected, and therefore, the model could not be tested."]},{"key":"dc:title","label":"Title","values":["A Genetic Test of a Model for Two Activities of Fushi Tarazu Protein in Drosophila Melanogaster"]}]}],"canonical_facts":{"dc:contributor.advisor":["Percival-Smith, Anthony"],"dc:creator":["Briek, Alaa"],"dc:date.accessioned":["2025-07-10T19:54:38Z"],"dc:date.available":["2025-07-10T19:54:38Z"],"dc:date.issued":["2016-04-19"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["The important pair-rule segmentation gene fushi tarazu (ftz) encodes a homeodomain (HD)-containing protein involved in the establishment of even-numbered parasegments during embryonic development. The D. melanogaster ftz is a derived homeotic selector (Hox) gene which lost its homeotic function during the evolution of arthropods. Genetic analyses have shown that FTZ has two distinct activities required during development: HD-dependent and HD-independent FTZ activities. The aim of this study was to test the interaction of the two FTZ activities proposed by Hyduk and Percival-Smith (1996), by generating site-specific mutant ftz alleles for intragenic complementation. CRISPR-mediated homology directed repair (HDR) was used to introduce engineered ftz alleles into the ftz locus. Subsequently, four ftz engineered alleles were constructed in vectors for reintroduction by Recombinase-mediated cassette exchange (RMCE). Despite using multiple approaches no CRISPR mediated HDR events were detected, and therefore, the model could not be tested."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/33671"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Drosophila melanogaster","pair rule genes","fushi tarazu","homeodomain","segmentation","CRISPR/Cas9."],"dc:title":["A Genetic Test of a Model for Two Activities of Fushi Tarazu Protein in Drosophila Melanogaster"],"dc:type":["thesis"],"thesis:degree_discipline":["Biology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:14Z"}