{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/33450"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/33450","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Treatment of Ischemia Reperfusion Injury with RNA Interference","abstract":"Ischemia reperfusion injury (IRI) occurs during transplantation and causes apoptosis and inflammation. The purpose of this research was to determine the effect of caspase-3, complement 3, and RelB gene silencing in the reduction of IRI using an in vitro model. LLC-PK1 cells were used along with antimycin A for the in vitro IRI model. Prior to exposure to antimycin A, cells were transfected with caspase-3, C3, and RelB small interfering RNA (siRNA) alone or in combination and then analyzed. The relative risk reduction of apoptosis in antimycin A treated cells with caspase-3 siRNA was 46.6% (p=0.019), RelB siRNA 42.8% (p=0.038), and complement 3 siRNA 13.9% (p=0.968). Combinations caspase-3 and RelB siRNAs showed significant changes, but were similar to transfection with caspase-3 and RelB alone. Caspase-3 and RelB siRNA are effective at reducing apoptosis in an in vitro model of IRI and will be used in future large animal studies.","abstract_html":"Ischemia reperfusion injury (IRI) occurs during transplantation and causes apoptosis and inflammation. The purpose of this research was to determine the effect of caspase-3, complement 3, and RelB gene silencing in the reduction of IRI using an in vitro model. LLC-PK1 cells were used along with antimycin A for the in vitro IRI model. Prior to exposure to antimycin A, cells were transfected with caspase-3, C3, and RelB small interfering RNA (siRNA) alone or in combination and then analyzed. The relative risk reduction of apoptosis in antimycin A treated cells with caspase-3 siRNA was 46.6% (p=0.019), RelB siRNA 42.8% (p=0.038), and complement 3 siRNA 13.9% (p=0.968). Combinations caspase-3 and RelB siRNAs showed significant changes, but were similar to transfection with caspase-3 and RelB alone. Caspase-3 and RelB siRNA are effective at reducing apoptosis in an in vitro model of IRI and will be used in future large animal studies.","abstract_has_math":false,"creators":["Zwiep, Terry M"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Surgery","degree_department":null,"school":null,"contributors":[],"advisors":["Doug Quan"],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-11-17","date_published":"2015-11-17","updated_at":"2026-07-27T21:56:20Z","subjects":["RNA Interference","siRNA","Ischemia Reperfusion Injury","Transplantation","Apoptosis","Hypoxia"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/33450","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Doug Quan"]},{"key":"dc:creator","label":"Author","values":["Zwiep, Terry M"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T19:52:35Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T19:52:35Z"]},{"key":"dc:date.issued","label":"Date","values":["2015-11-17"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Surgery"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["RNA Interference","siRNA","Ischemia Reperfusion Injury","Transplantation","Apoptosis","Hypoxia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/33450"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Ischemia reperfusion injury (IRI) occurs during transplantation and causes apoptosis and inflammation. The purpose of this research was to determine the effect of caspase-3, complement 3, and RelB gene silencing in the reduction of IRI using an in vitro model. LLC-PK1 cells were used along with antimycin A for the in vitro IRI model. Prior to exposure to antimycin A, cells were transfected with caspase-3, C3, and RelB small interfering RNA (siRNA) alone or in combination and then analyzed. The relative risk reduction of apoptosis in antimycin A treated cells with caspase-3 siRNA was 46.6% (p=0.019), RelB siRNA 42.8% (p=0.038), and complement 3 siRNA 13.9% (p=0.968). Combinations caspase-3 and RelB siRNAs showed significant changes, but were similar to transfection with caspase-3 and RelB alone. Caspase-3 and RelB siRNA are effective at reducing apoptosis in an in vitro model of IRI and will be used in future large animal studies."]},{"key":"dc:title","label":"Title","values":["Treatment of Ischemia Reperfusion Injury with RNA Interference"]}]}],"canonical_facts":{"dc:contributor.advisor":["Doug Quan"],"dc:creator":["Zwiep, Terry M"],"dc:date.accessioned":["2025-07-10T19:52:35Z"],"dc:date.available":["2025-07-10T19:52:35Z"],"dc:date.issued":["2015-11-17"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Ischemia reperfusion injury (IRI) occurs during transplantation and causes apoptosis and inflammation. The purpose of this research was to determine the effect of caspase-3, complement 3, and RelB gene silencing in the reduction of IRI using an in vitro model. LLC-PK1 cells were used along with antimycin A for the in vitro IRI model. Prior to exposure to antimycin A, cells were transfected with caspase-3, C3, and RelB small interfering RNA (siRNA) alone or in combination and then analyzed. The relative risk reduction of apoptosis in antimycin A treated cells with caspase-3 siRNA was 46.6% (p=0.019), RelB siRNA 42.8% (p=0.038), and complement 3 siRNA 13.9% (p=0.968). Combinations caspase-3 and RelB siRNAs showed significant changes, but were similar to transfection with caspase-3 and RelB alone. Caspase-3 and RelB siRNA are effective at reducing apoptosis in an in vitro model of IRI and will be used in future large animal studies."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/33450"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["RNA Interference","siRNA","Ischemia Reperfusion Injury","Transplantation","Apoptosis","Hypoxia"],"dc:title":["Treatment of Ischemia Reperfusion Injury with RNA Interference"],"dc:type":["thesis"],"thesis:degree_discipline":["Surgery"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:20Z"}