{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/32725"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/32725","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Neuronal Nitric Oxide synthase regulates the expression of EAAT4 and GAD65/67 in the murine cerebellum","abstract":"The cerebellum is a brain structure that is responsible for coordinating voluntary movement such as balance, coordination, and posture. Purkinje neurons (PNs) are the sole GABAergic output from the cerebellar cortex and receive major excitatory inputs via parallel fibers (PFs) that produce high levels of nitric oxide (NO) via neuronal nitric oxide synthase (nNOS). Previous studies demonstrate that nNOS/NO signaling is crucial for PF-PN synaptic transmission. This study sought to examine nNOS/NO signaling regulation of excitatory amino acid transporter 4 (EAAT4) on PN dendrites and glutamic acid decarboxylase (GAD) expression/function between sexes by using wildtype (WT) and nNOS-/- mice at different postnatal developmental time points. This study revealed that there are gene- and sex-dependent differences in GABA concentration in the cerebellum during early postnatal development. Additionally, there is decreased EAAT4 expression in male nNOS-/- mice. Lastly, there is decreased GAD65/67 expression in both male and female nNOS-/- nice compared to age-matched WT mice. Our results provide new knowledge of NO regulation of cerebellar synaptic transmission, which may advance the development of cerebellar ataxia therapeutics.","abstract_html":"The cerebellum is a brain structure that is responsible for coordinating voluntary movement such as balance, coordination, and posture. Purkinje neurons (PNs) are the sole GABAergic output from the cerebellar cortex and receive major excitatory inputs via parallel fibers (PFs) that produce high levels of nitric oxide (NO) via neuronal nitric oxide synthase (nNOS). Previous studies demonstrate that nNOS/NO signaling is crucial for PF-PN synaptic transmission. This study sought to examine nNOS/NO signaling regulation of excitatory amino acid transporter 4 (EAAT4) on PN dendrites and glutamic acid decarboxylase (GAD) expression/function between sexes by using wildtype (WT) and nNOS-/- mice at different postnatal developmental time points. This study revealed that there are gene- and sex-dependent differences in GABA concentration in the cerebellum during early postnatal development. Additionally, there is decreased EAAT4 expression in male nNOS-/- mice. Lastly, there is decreased GAD65/67 expression in both male and female nNOS-/- nice compared to age-matched WT mice. Our results provide new knowledge of NO regulation of cerebellar synaptic transmission, which may advance the development of cerebellar ataxia therapeutics.","abstract_has_math":false,"creators":["Jassal, Gurneet"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Physiology and Pharmacology","degree_department":null,"school":null,"contributors":[],"advisors":["Wei-Yang Lu"],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-12-16","date_published":"2022-12-16","updated_at":"2026-07-27T21:56:14Z","subjects":["Cerebellum","Neuronal Nitric Oxide Synthase","Nitric Oxide","Purkinje Neuron","Excitotoxicity"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/32725","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Wei-Yang Lu"]},{"key":"dc:creator","label":"Author","values":["Jassal, Gurneet"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T19:35:31Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T19:35:31Z"]},{"key":"dc:date.issued","label":"Date","values":["2022-12-16"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology and Pharmacology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cerebellum","Neuronal Nitric Oxide Synthase","Nitric Oxide","Purkinje Neuron","Excitotoxicity"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/32725"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["The cerebellum is a brain structure that is responsible for coordinating voluntary movement such as balance, coordination, and posture. Purkinje neurons (PNs) are the sole GABAergic output from the cerebellar cortex and receive major excitatory inputs via parallel fibers (PFs) that produce high levels of nitric oxide (NO) via neuronal nitric oxide synthase (nNOS). Previous studies demonstrate that nNOS/NO signaling is crucial for PF-PN synaptic transmission. This study sought to examine nNOS/NO signaling regulation of excitatory amino acid transporter 4 (EAAT4) on PN dendrites and glutamic acid decarboxylase (GAD) expression/function between sexes by using wildtype (WT) and nNOS-/- mice at different postnatal developmental time points. This study revealed that there are gene- and sex-dependent differences in GABA concentration in the cerebellum during early postnatal development. Additionally, there is decreased EAAT4 expression in male nNOS-/- mice. Lastly, there is decreased GAD65/67 expression in both male and female nNOS-/- nice compared to age-matched WT mice. Our results provide new knowledge of NO regulation of cerebellar synaptic transmission, which may advance the development of cerebellar ataxia therapeutics."]},{"key":"dc:title","label":"Title","values":["Neuronal Nitric Oxide synthase regulates the expression of EAAT4 and GAD65/67 in the murine cerebellum"]}]}],"canonical_facts":{"dc:contributor.advisor":["Wei-Yang Lu"],"dc:creator":["Jassal, Gurneet"],"dc:date.accessioned":["2025-07-10T19:35:31Z"],"dc:date.available":["2025-07-10T19:35:31Z"],"dc:date.issued":["2022-12-16"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["The cerebellum is a brain structure that is responsible for coordinating voluntary movement such as balance, coordination, and posture. Purkinje neurons (PNs) are the sole GABAergic output from the cerebellar cortex and receive major excitatory inputs via parallel fibers (PFs) that produce high levels of nitric oxide (NO) via neuronal nitric oxide synthase (nNOS). Previous studies demonstrate that nNOS/NO signaling is crucial for PF-PN synaptic transmission. This study sought to examine nNOS/NO signaling regulation of excitatory amino acid transporter 4 (EAAT4) on PN dendrites and glutamic acid decarboxylase (GAD) expression/function between sexes by using wildtype (WT) and nNOS-/- mice at different postnatal developmental time points. This study revealed that there are gene- and sex-dependent differences in GABA concentration in the cerebellum during early postnatal development. Additionally, there is decreased EAAT4 expression in male nNOS-/- mice. Lastly, there is decreased GAD65/67 expression in both male and female nNOS-/- nice compared to age-matched WT mice. Our results provide new knowledge of NO regulation of cerebellar synaptic transmission, which may advance the development of cerebellar ataxia therapeutics."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/32725"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Cerebellum","Neuronal Nitric Oxide Synthase","Nitric Oxide","Purkinje Neuron","Excitotoxicity"],"dc:title":["Neuronal Nitric Oxide synthase regulates the expression of EAAT4 and GAD65/67 in the murine cerebellum"],"dc:type":["thesis"],"thesis:degree_discipline":["Physiology and Pharmacology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:14Z"}