{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/31497"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/31497","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Effects of ergothioneine on endothelial cell and macrophage characteristics, and markers of atherosclerosis risk under high lipid conditions","abstract":"Ergothioneine (EGT) is an antioxidant and potential anti-inflammatory molecule that may have protective effects against the lipid-induced oxidative stress and inflammation that occurs in the vasculature of individuals with obesity and metabolic syndrome. This thesis addresses the hypothesis that EGT supplementation decreases endothelial cell dysfunction and macrophage inflammatory characteristics under high lipid conditions, and markers of atherosclerotic risk in diet-induced obese mice. Results showed that EGT did not influence endothelial tube formation or stability but reduced nitric oxide production in endothelial cells. Moreover, EGT decreased the production of reactive oxygen species, and potentially nitric oxide, by macrophages. In diet-induced obese mice with characteristics of metabolic syndrome, EGT did not influence circulating lipid, lipoprotein and glucose concentrations which are markers of atherosclerotic risk. In conclusion, EGT has moderate effects on vascular cells in vitro, and does not influence blood glucose and lipid markers of vascular disease risk in diet-induced obese mice.","abstract_html":"Ergothioneine (EGT) is an antioxidant and potential anti-inflammatory molecule that may have protective effects against the lipid-induced oxidative stress and inflammation that occurs in the vasculature of individuals with obesity and metabolic syndrome. This thesis addresses the hypothesis that EGT supplementation decreases endothelial cell dysfunction and macrophage inflammatory characteristics under high lipid conditions, and markers of atherosclerotic risk in diet-induced obese mice. Results showed that EGT did not influence endothelial tube formation or stability but reduced nitric oxide production in endothelial cells. Moreover, EGT decreased the production of reactive oxygen species, and potentially nitric oxide, by macrophages. In diet-induced obese mice with characteristics of metabolic syndrome, EGT did not influence circulating lipid, lipoprotein and glucose concentrations which are markers of atherosclerotic risk. In conclusion, EGT has moderate effects on vascular cells in vitro, and does not influence blood glucose and lipid markers of vascular disease risk in diet-induced obese mice.","abstract_has_math":false,"creators":["Lam-Sidun, Daniel"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Physiology and Pharmacology","degree_department":null,"school":null,"contributors":[],"advisors":["Borradaile, Nica M."],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-08-26","date_published":"2021-08-26","updated_at":"2026-07-27T21:55:54Z","subjects":["ergothioneine","antioxidant","obesity","vascular disease","endothelial cells","macrophages"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/31497","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Borradaile, Nica M."]},{"key":"dc:creator","label":"Author","values":["Lam-Sidun, Daniel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T19:19:45Z"]},{"key":"dc:date.issued","label":"Date","values":["2021-08-26"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology and Pharmacology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ergothioneine","antioxidant","obesity","vascular disease","endothelial cells","macrophages"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/31497"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Ergothioneine (EGT) is an antioxidant and potential anti-inflammatory molecule that may have protective effects against the lipid-induced oxidative stress and inflammation that occurs in the vasculature of individuals with obesity and metabolic syndrome. This thesis addresses the hypothesis that EGT supplementation decreases endothelial cell dysfunction and macrophage inflammatory characteristics under high lipid conditions, and markers of atherosclerotic risk in diet-induced obese mice. Results showed that EGT did not influence endothelial tube formation or stability but reduced nitric oxide production in endothelial cells. Moreover, EGT decreased the production of reactive oxygen species, and potentially nitric oxide, by macrophages. In diet-induced obese mice with characteristics of metabolic syndrome, EGT did not influence circulating lipid, lipoprotein and glucose concentrations which are markers of atherosclerotic risk. In conclusion, EGT has moderate effects on vascular cells in vitro, and does not influence blood glucose and lipid markers of vascular disease risk in diet-induced obese mice."]},{"key":"dc:title","label":"Title","values":["Effects of ergothioneine on endothelial cell and macrophage characteristics, and markers of atherosclerosis risk under high lipid conditions"]}]}],"canonical_facts":{"dc:contributor.advisor":["Borradaile, Nica M."],"dc:creator":["Lam-Sidun, Daniel"],"dc:date.accessioned":["2025-07-10T19:19:45Z"],"dc:date.issued":["2021-08-26"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Ergothioneine (EGT) is an antioxidant and potential anti-inflammatory molecule that may have protective effects against the lipid-induced oxidative stress and inflammation that occurs in the vasculature of individuals with obesity and metabolic syndrome. This thesis addresses the hypothesis that EGT supplementation decreases endothelial cell dysfunction and macrophage inflammatory characteristics under high lipid conditions, and markers of atherosclerotic risk in diet-induced obese mice. Results showed that EGT did not influence endothelial tube formation or stability but reduced nitric oxide production in endothelial cells. Moreover, EGT decreased the production of reactive oxygen species, and potentially nitric oxide, by macrophages. In diet-induced obese mice with characteristics of metabolic syndrome, EGT did not influence circulating lipid, lipoprotein and glucose concentrations which are markers of atherosclerotic risk. In conclusion, EGT has moderate effects on vascular cells in vitro, and does not influence blood glucose and lipid markers of vascular disease risk in diet-induced obese mice."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/31497"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["ergothioneine","antioxidant","obesity","vascular disease","endothelial cells","macrophages"],"dc:title":["Effects of ergothioneine on endothelial cell and macrophage characteristics, and markers of atherosclerosis risk under high lipid conditions"],"dc:type":["thesis"],"thesis:degree_discipline":["Physiology and Pharmacology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:55:54Z"}