{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/28665"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/28665","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Identification and Characterization of Novel Mycotoxin Degrading Enzymes","abstract":"Plant pathogenic fungi throughout the agricultural supply chain produce mycotoxins, which are harmful secondary metabolites that aid fungal colonization and contaminate food and feed. Consuming mycotoxin-contaminated products poses health risks to humans and animals. While several strategies to limit mycotoxin contamination exist, enzymatic biotransformation of mycotoxins into non-toxic forms offers a potentially safe, specific, and robust solution to manage mycotoxins. To identify novel mycotoxin degradation activities, an in-house developed discovery-based pipeline was employed to culture various mixed microbial communities with mycotoxins. Using the pipeline, a novel thermophilic bacterium that degrades Ochratoxin A was discovered. Additionally, a zearalenone hydrolase previously identified with the pipeline was biochemically characterized and demonstrated to hydrolyze additional resorcylic acid lactones beyond zearalenone, including radicicol. Radicicol inhibits the activity of Heat Shock Protein 90, however following enzymatic treatment, this activity was significantly attenuated. Identifying mycotoxin degradation activity supports continued agricultural production while preserving economic and health interests.","abstract_html":"Plant pathogenic fungi throughout the agricultural supply chain produce mycotoxins, which are harmful secondary metabolites that aid fungal colonization and contaminate food and feed. Consuming mycotoxin-contaminated products poses health risks to humans and animals. While several strategies to limit mycotoxin contamination exist, enzymatic biotransformation of mycotoxins into non-toxic forms offers a potentially safe, specific, and robust solution to manage mycotoxins. To identify novel mycotoxin degradation activities, an in-house developed discovery-based pipeline was employed to culture various mixed microbial communities with mycotoxins. Using the pipeline, a novel thermophilic bacterium that degrades Ochratoxin A was discovered. Additionally, a zearalenone hydrolase previously identified with the pipeline was biochemically characterized and demonstrated to hydrolyze additional resorcylic acid lactones beyond zearalenone, including radicicol. Radicicol inhibits the activity of Heat Shock Protein 90, however following enzymatic treatment, this activity was significantly attenuated. Identifying mycotoxin degradation activity supports continued agricultural production while preserving economic and health interests.","abstract_has_math":false,"creators":["Hendricks, Kyle N"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Garnham, Christopher P.","Thorn, Greg"],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-09-16","date_published":"2024-09-16","updated_at":"2026-07-27T21:55:59Z","subjects":["Mycotoxins","Zearalenone","Radicicol","Ochratoxin A","Biotransformation","Aeromicrobium","Chelatococcus","Hydrolase","Peptidase","Agriculture","HSP90"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/28665","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Garnham, Christopher P.","Thorn, Greg"]},{"key":"dc:creator","label":"Author","values":["Hendricks, Kyle N"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T16:13:47Z"]},{"key":"dc:date.issued","label":"Date","values":["2024-09-16"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Mycotoxins","Zearalenone","Radicicol","Ochratoxin A","Biotransformation","Aeromicrobium","Chelatococcus","Hydrolase","Peptidase","Agriculture","HSP90"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/28665"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Plant pathogenic fungi throughout the agricultural supply chain produce mycotoxins, which are harmful secondary metabolites that aid fungal colonization and contaminate food and feed. Consuming mycotoxin-contaminated products poses health risks to humans and animals. While several strategies to limit mycotoxin contamination exist, enzymatic biotransformation of mycotoxins into non-toxic forms offers a potentially safe, specific, and robust solution to manage mycotoxins. To identify novel mycotoxin degradation activities, an in-house developed discovery-based pipeline was employed to culture various mixed microbial communities with mycotoxins. Using the pipeline, a novel thermophilic bacterium that degrades Ochratoxin A was discovered. Additionally, a zearalenone hydrolase previously identified with the pipeline was biochemically characterized and demonstrated to hydrolyze additional resorcylic acid lactones beyond zearalenone, including radicicol. Radicicol inhibits the activity of Heat Shock Protein 90, however following enzymatic treatment, this activity was significantly attenuated. Identifying mycotoxin degradation activity supports continued agricultural production while preserving economic and health interests."]},{"key":"dc:title","label":"Title","values":["Identification and Characterization of Novel Mycotoxin Degrading Enzymes"]}]}],"canonical_facts":{"dc:contributor.advisor":["Garnham, Christopher P.","Thorn, Greg"],"dc:creator":["Hendricks, Kyle N"],"dc:date.accessioned":["2025-07-10T16:13:47Z"],"dc:date.issued":["2024-09-16"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Plant pathogenic fungi throughout the agricultural supply chain produce mycotoxins, which are harmful secondary metabolites that aid fungal colonization and contaminate food and feed. Consuming mycotoxin-contaminated products poses health risks to humans and animals. While several strategies to limit mycotoxin contamination exist, enzymatic biotransformation of mycotoxins into non-toxic forms offers a potentially safe, specific, and robust solution to manage mycotoxins. To identify novel mycotoxin degradation activities, an in-house developed discovery-based pipeline was employed to culture various mixed microbial communities with mycotoxins. Using the pipeline, a novel thermophilic bacterium that degrades Ochratoxin A was discovered. Additionally, a zearalenone hydrolase previously identified with the pipeline was biochemically characterized and demonstrated to hydrolyze additional resorcylic acid lactones beyond zearalenone, including radicicol. Radicicol inhibits the activity of Heat Shock Protein 90, however following enzymatic treatment, this activity was significantly attenuated. 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