{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/28345"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/28345","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"The Effects of Tracheal Occlusion on Wnt Signaling in a Rabbit Model of Congenital Diaphragmatic Hernia","abstract":"Purpose: Tracheal occlusion (TO) reverses pulmonary hypoplasia (PH) in congenital diaphragmatic hernia (CDH), but its effect on epithelial-mesenchymal transition (EMT) in lung development remains poorly understood. The purpose of this study was to a) confirm the CDH rabbit model produced PH which was reversed by TO and b) determine the effects of CDH +/- TO on EMT pathways. Methods: CDH was created at 23 days, TO at 28 days and lung collection at 31 days gestation in fetal rabbits. Lung body weight ratio (LBWR), mean terminal bronchiole density (MTBD), and expression of mRNA and micro-RNA was determined. Results: Fifteen CDH, 15 CDH+TO, 6 sham CDH, and 15 controls were included in the study. LBWR was low in CDH while CDH+TO was similar to controls. MTBD was higher in CDH fetuses and restored to control levels in CDH+TO. miR-33 and MKI67 were increased following TO, while Lgl1 was decreased in CDH+TO. Conclusion: TO reversed PH and stimulated early Wnt signaling in CDH fetal rabbits.","abstract_html":"Purpose: Tracheal occlusion (TO) reverses pulmonary hypoplasia (PH) in congenital diaphragmatic hernia (CDH), but its effect on epithelial-mesenchymal transition (EMT) in lung development remains poorly understood. The purpose of this study was to a) confirm the CDH rabbit model produced PH which was reversed by TO and b) determine the effects of CDH +/- TO on EMT pathways. Methods: CDH was created at 23 days, TO at 28 days and lung collection at 31 days gestation in fetal rabbits. Lung body weight ratio (LBWR), mean terminal bronchiole density (MTBD), and expression of mRNA and micro-RNA was determined. Results: Fifteen CDH, 15 CDH+TO, 6 sham CDH, and 15 controls were included in the study. LBWR was low in CDH while CDH+TO was similar to controls. MTBD was higher in CDH fetuses and restored to control levels in CDH+TO. miR-33 and MKI67 were increased following TO, while Lgl1 was decreased in CDH+TO. Conclusion: TO reversed PH and stimulated early Wnt signaling in CDH fetal rabbits.","abstract_has_math":false,"creators":["Mudri, Martina M"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Surgery","degree_department":null,"school":null,"contributors":[],"advisors":["Bütter, Andreana","Regnault, Timothy R.H."],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-09-05","date_published":"2018-09-05","updated_at":"2026-07-27T21:56:07Z","subjects":["Congenital diaphragmatic hernia (CDH)","tracheal occlusion (TO)","epithelial-mesenchymal transition (EMT)","lung development","Wnt signaling","micro-RNA"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/28345","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Bütter, Andreana","Regnault, Timothy R.H."]},{"key":"dc:creator","label":"Author","values":["Mudri, Martina M"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T16:10:14Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T16:10:14Z"]},{"key":"dc:date.issued","label":"Date","values":["2018-09-05"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Surgery"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Congenital diaphragmatic hernia (CDH)","tracheal occlusion (TO)","epithelial-mesenchymal transition (EMT)","lung development","Wnt signaling","micro-RNA"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/28345"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Purpose: Tracheal occlusion (TO) reverses pulmonary hypoplasia (PH) in congenital diaphragmatic hernia (CDH), but its effect on epithelial-mesenchymal transition (EMT) in lung development remains poorly understood. The purpose of this study was to a) confirm the CDH rabbit model produced PH which was reversed by TO and b) determine the effects of CDH +/- TO on EMT pathways. Methods: CDH was created at 23 days, TO at 28 days and lung collection at 31 days gestation in fetal rabbits. Lung body weight ratio (LBWR), mean terminal bronchiole density (MTBD), and expression of mRNA and micro-RNA was determined. Results: Fifteen CDH, 15 CDH+TO, 6 sham CDH, and 15 controls were included in the study. LBWR was low in CDH while CDH+TO was similar to controls. MTBD was higher in CDH fetuses and restored to control levels in CDH+TO. miR-33 and MKI67 were increased following TO, while Lgl1 was decreased in CDH+TO. Conclusion: TO reversed PH and stimulated early Wnt signaling in CDH fetal rabbits."]},{"key":"dc:title","label":"Title","values":["The Effects of Tracheal Occlusion on Wnt Signaling in a Rabbit Model of Congenital Diaphragmatic Hernia"]}]}],"canonical_facts":{"dc:contributor.advisor":["Bütter, Andreana","Regnault, Timothy R.H."],"dc:creator":["Mudri, Martina M"],"dc:date.accessioned":["2025-07-10T16:10:14Z"],"dc:date.available":["2025-07-10T16:10:14Z"],"dc:date.issued":["2018-09-05"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Purpose: Tracheal occlusion (TO) reverses pulmonary hypoplasia (PH) in congenital diaphragmatic hernia (CDH), but its effect on epithelial-mesenchymal transition (EMT) in lung development remains poorly understood. The purpose of this study was to a) confirm the CDH rabbit model produced PH which was reversed by TO and b) determine the effects of CDH +/- TO on EMT pathways. Methods: CDH was created at 23 days, TO at 28 days and lung collection at 31 days gestation in fetal rabbits. Lung body weight ratio (LBWR), mean terminal bronchiole density (MTBD), and expression of mRNA and micro-RNA was determined. Results: Fifteen CDH, 15 CDH+TO, 6 sham CDH, and 15 controls were included in the study. LBWR was low in CDH while CDH+TO was similar to controls. MTBD was higher in CDH fetuses and restored to control levels in CDH+TO. miR-33 and MKI67 were increased following TO, while Lgl1 was decreased in CDH+TO. Conclusion: TO reversed PH and stimulated early Wnt signaling in CDH fetal rabbits."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/28345"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Congenital diaphragmatic hernia (CDH)","tracheal occlusion (TO)","epithelial-mesenchymal transition (EMT)","lung development","Wnt signaling","micro-RNA"],"dc:title":["The Effects of Tracheal Occlusion on Wnt Signaling in a Rabbit Model of Congenital Diaphragmatic Hernia"],"dc:type":["thesis"],"thesis:degree_discipline":["Surgery"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:07Z"}