{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/27917"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/27917","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Detection and Imaging of Neurotransmitters in Rodent Brain Tissue Using FMP-10 and ZnO Nanoparticle Matrices with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry","abstract":"Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an analytical technique used to visualize the spatial distribution of molecules within biological samples. In our lab, we employ two complementary matrices, 2-fluoro-1-methyl pyridinium (FMP-10) and zinc oxide nanoparticles (ZnO NP), to image neurotransmitters in rodent brain tissue. FMP-10 facilitates on-tissue chemical derivatization to detect 17 neurochemicals, notably dopamine, γ-aminobutyric acid (GABA), serotonin, and norepinephrine. ZnO NP detects 9 neurochemicals, including glutamate and glutamine, which are undetectable by FMP-10. We applied both matrices to investigate neurochemical changes in male and female adolescent rats administered edible Δ-9-tetrahydrocannabinol (THC). Our MALDI MSI data revealed significant downregulation of dopamine, GABA, glutamate, and glutamine levels in the prefrontal cortex and nucleus accumbens of THC-treated males and females. To improve the quantitative accuracy of our MALDI MSI analysis, we evaluated deuterated GABA (GABA-d6) as an internal standard to achieve precise and reproducible quantitation of molecules.","abstract_html":"Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an analytical technique used to visualize the spatial distribution of molecules within biological samples. In our lab, we employ two complementary matrices, 2-fluoro-1-methyl pyridinium (FMP-10) and zinc oxide nanoparticles (ZnO NP), to image neurotransmitters in rodent brain tissue. FMP-10 facilitates on-tissue chemical derivatization to detect 17 neurochemicals, notably dopamine, γ-aminobutyric acid (GABA), serotonin, and norepinephrine. ZnO NP detects 9 neurochemicals, including glutamate and glutamine, which are undetectable by FMP-10. We applied both matrices to investigate neurochemical changes in male and female adolescent rats administered edible Δ-9-tetrahydrocannabinol (THC). Our MALDI MSI data revealed significant downregulation of dopamine, GABA, glutamate, and glutamine levels in the prefrontal cortex and nucleus accumbens of THC-treated males and females. To improve the quantitative accuracy of our MALDI MSI analysis, we evaluated deuterated GABA (GABA-d6) as an internal standard to achieve precise and reproducible quantitation of molecules.","abstract_has_math":false,"creators":["Pusparajah, Mathusha"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":["Yeung, Ken"],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-09-12","date_published":"2024-09-12","updated_at":"2026-07-27T21:56:20Z","subjects":["MALDI","mass spectrometry imaging","FMP-10","ZnO nanoparticles","rodent brain","neurotransmitters"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/27917","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Yeung, Ken"]},{"key":"dc:creator","label":"Author","values":["Pusparajah, Mathusha"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T15:33:59Z"]},{"key":"dc:date.issued","label":"Date","values":["2024-09-12"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["MALDI","mass spectrometry imaging","FMP-10","ZnO nanoparticles","rodent brain","neurotransmitters"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/27917"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an analytical technique used to visualize the spatial distribution of molecules within biological samples. In our lab, we employ two complementary matrices, 2-fluoro-1-methyl pyridinium (FMP-10) and zinc oxide nanoparticles (ZnO NP), to image neurotransmitters in rodent brain tissue. FMP-10 facilitates on-tissue chemical derivatization to detect 17 neurochemicals, notably dopamine, γ-aminobutyric acid (GABA), serotonin, and norepinephrine. ZnO NP detects 9 neurochemicals, including glutamate and glutamine, which are undetectable by FMP-10. We applied both matrices to investigate neurochemical changes in male and female adolescent rats administered edible Δ-9-tetrahydrocannabinol (THC). Our MALDI MSI data revealed significant downregulation of dopamine, GABA, glutamate, and glutamine levels in the prefrontal cortex and nucleus accumbens of THC-treated males and females. To improve the quantitative accuracy of our MALDI MSI analysis, we evaluated deuterated GABA (GABA-d6) as an internal standard to achieve precise and reproducible quantitation of molecules."]},{"key":"dc:title","label":"Title","values":["Detection and Imaging of Neurotransmitters in Rodent Brain Tissue Using FMP-10 and ZnO Nanoparticle Matrices with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry"]}]}],"canonical_facts":{"dc:contributor.advisor":["Yeung, Ken"],"dc:creator":["Pusparajah, Mathusha"],"dc:date.accessioned":["2025-07-10T15:33:59Z"],"dc:date.issued":["2024-09-12"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an analytical technique used to visualize the spatial distribution of molecules within biological samples. In our lab, we employ two complementary matrices, 2-fluoro-1-methyl pyridinium (FMP-10) and zinc oxide nanoparticles (ZnO NP), to image neurotransmitters in rodent brain tissue. FMP-10 facilitates on-tissue chemical derivatization to detect 17 neurochemicals, notably dopamine, γ-aminobutyric acid (GABA), serotonin, and norepinephrine. ZnO NP detects 9 neurochemicals, including glutamate and glutamine, which are undetectable by FMP-10. We applied both matrices to investigate neurochemical changes in male and female adolescent rats administered edible Δ-9-tetrahydrocannabinol (THC). Our MALDI MSI data revealed significant downregulation of dopamine, GABA, glutamate, and glutamine levels in the prefrontal cortex and nucleus accumbens of THC-treated males and females. To improve the quantitative accuracy of our MALDI MSI analysis, we evaluated deuterated GABA (GABA-d6) as an internal standard to achieve precise and reproducible quantitation of molecules."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/27917"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["MALDI","mass spectrometry imaging","FMP-10","ZnO nanoparticles","rodent brain","neurotransmitters"],"dc:title":["Detection and Imaging of Neurotransmitters in Rodent Brain Tissue Using FMP-10 and ZnO Nanoparticle Matrices with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry"],"dc:type":["thesis"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:20Z"}