{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/27791"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/27791","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters","abstract":"Phenylalanine is an important aromatic amino acid synthesized by higher plants, and is a major component of numerous specialized metabolites including structural components, pigments, and defense compounds. The last step in the synthesis of phenylalanine is catalyzed by an enzyme called AROGENATE DEHYDRATASE, of which there are six different isoenzymes encoded by the Arabidopsis genome. All six have specialized roles within the plant, and are differentially expressed during development and under stressful conditions. To deduce the specialized role of each ADT, unique patterns of regulatory motifs were identified for all six ADT promoters, as well as corresponding transcription factors with similar expression profiles to each enzyme. Seven stable transgenic Arabidopsis lines were also generated using ADT promoter-eGFP/GUS constructs to test expression in all tissues during development, and under stressful conditions.","abstract_html":"Phenylalanine is an important aromatic amino acid synthesized by higher plants, and is a major component of numerous specialized metabolites including structural components, pigments, and defense compounds. The last step in the synthesis of phenylalanine is catalyzed by an enzyme called AROGENATE DEHYDRATASE, of which there are six different isoenzymes encoded by the Arabidopsis genome. All six have specialized roles within the plant, and are differentially expressed during development and under stressful conditions. To deduce the specialized role of each ADT, unique patterns of regulatory motifs were identified for all six ADT promoters, as well as corresponding transcription factors with similar expression profiles to each enzyme. Seven stable transgenic Arabidopsis lines were also generated using ADT promoter-eGFP/GUS constructs to test expression in all tissues during development, and under stressful conditions.","abstract_has_math":false,"creators":["Cornelius, Emily J"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Susanne E. Kohalmi"],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-11-03","date_published":"2017-11-03","updated_at":"2026-07-27T21:56:01Z","subjects":["Phenylalanine","specialized metabolism","arogenate dehydratase","cis regulatory motif","co-expression","transcriptional regulation"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/27791","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Susanne E. 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In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Phenylalanine is an important aromatic amino acid synthesized by higher plants, and is a major component of numerous specialized metabolites including structural components, pigments, and defense compounds. The last step in the synthesis of phenylalanine is catalyzed by an enzyme called AROGENATE DEHYDRATASE, of which there are six different isoenzymes encoded by the Arabidopsis genome. All six have specialized roles within the plant, and are differentially expressed during development and under stressful conditions. To deduce the specialized role of each ADT, unique patterns of regulatory motifs were identified for all six ADT promoters, as well as corresponding transcription factors with similar expression profiles to each enzyme. Seven stable transgenic Arabidopsis lines were also generated using ADT promoter-eGFP/GUS constructs to test expression in all tissues during development, and under stressful conditions."]},{"key":"dc:title","label":"Title","values":["Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters"]}]}],"canonical_facts":{"dc:contributor.advisor":["Susanne E. Kohalmi"],"dc:creator":["Cornelius, Emily J"],"dc:date.accessioned":["2025-07-10T15:32:47Z"],"dc:date.available":["2025-07-10T15:32:47Z"],"dc:date.issued":["2017-11-03"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Phenylalanine is an important aromatic amino acid synthesized by higher plants, and is a major component of numerous specialized metabolites including structural components, pigments, and defense compounds. The last step in the synthesis of phenylalanine is catalyzed by an enzyme called AROGENATE DEHYDRATASE, of which there are six different isoenzymes encoded by the Arabidopsis genome. All six have specialized roles within the plant, and are differentially expressed during development and under stressful conditions. To deduce the specialized role of each ADT, unique patterns of regulatory motifs were identified for all six ADT promoters, as well as corresponding transcription factors with similar expression profiles to each enzyme. Seven stable transgenic Arabidopsis lines were also generated using ADT promoter-eGFP/GUS constructs to test expression in all tissues during development, and under stressful conditions."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/27791"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Phenylalanine","specialized metabolism","arogenate dehydratase","cis regulatory motif","co-expression","transcriptional regulation"],"dc:title":["Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters"],"dc:type":["thesis"],"thesis:degree_discipline":["Biology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:01Z"}