Structural And Chemical Organization Of The Rat Interpeduncular Nucleus
type: CreativeWork
description: The structural and chemical organization of the rat interpeduncular nucleus (IPN) was examined employing wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) and immunohistochemistry at the light and electron microscope level. The topographical and ultrastructural arrangements of habenular efferents innervating the IPN were studied using stereotaxic placements of WGA-HRP. The medial habenula provided a prominent afferent input to the many IPN subnuclei, with the lateral and rostral subnuclei receiving a predominantly ipsilateral input, and the intermediate, central and dorsomedial subnuclei receiving a symmetrical projection. Habenular terminals formed distinct axodendritic synapses in the rostral, lateral, intermediate and central subnuclei.;Projections of the fasciculus retroflexus (FR) were stained immunohistochemically for choline acetyltransferase (ChAT) or substance P (SP). These inputs were topographically organized among the IPN subnuclei, such that ChAT-positive axons innervated the rostral, intermediate and central subnuclei, and SP-positive inputs terminated in the lateral, dorsomedial and rostral subnuclei. Characteristic axodendritic synapses of the intermediate, central and rostral subnuclei were composed of ChAT-positive terminals, and those in the lateral, rostral and dorsomedial subnuclei were composed of SP-positive terminals.;A prominent population of neurons and an extensive plexus of axons and terminals stained immunohistochemically for glutamic acid decarboxylase (GAD) were present in the rostral, lateral, central and intermediate subnuclei. Axodendritic and axosomatic synapses possessing GAD immunoreactivity pre - and/or postsynaptically were evident in these subnuclei. GAD-immunoreactive dendrites in the intermediate, central and rostral subnuclei also received ChAT-positive terminals, and those in the lateral subnucleus received SF-positive terminals. SP-positive dendrites in the rostral subnucleus were also in receipt of ChA…
abstract: The structural and chemical organization of the rat interpeduncular nucleus (IPN) was examined employing wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) and immunohistochemistry at the light and electron microscope level. The topographical and ultrastructural arrangements of habenular efferents innervating the IPN were studied using stereotaxic placements of WGA-HRP. The medial habenula provided a prominent afferent input to the many IPN subnuclei, with the lateral and rostral subnuclei receiving a predominantly ipsilateral input, and the intermediate, central and dorsomedial subnuclei receiving a symmetrical projection. Habenular terminals formed distinct axodendritic synapses in the rostral, lateral, intermediate and central subnuclei.;Projections of the fasciculus retroflexus (FR) were stained immunohistochemically for choline acetyltransferase (ChAT) or substance P (SP). These inputs were topographically organized among the IPN subnuclei, such that ChAT-positive axons innervated the rostral, intermediate and central subnuclei, and SP-positive inputs terminated in the lateral, dorsomedial and rostral subnuclei. Characteristic axodendritic synapses of the intermediate, central and rostral subnuclei were composed of ChAT-positive terminals, and those in the lateral, rostral and dorsomedial subnuclei were composed of SP-positive terminals.;A prominent population of neurons and an extensive plexus of axons and terminals stained immunohistochemically for glutamic acid decarboxylase (GAD) were present in the rostral, lateral, central and intermediate subnuclei. Axodendritic and axosomatic synapses possessing GAD immunoreactivity pre - and/or postsynaptically were evident in these subnuclei. GAD-immunoreactive dendrites in the intermediate, central and rostral subnuclei also received ChAT-positive terminals, and those in the lateral subnucleus received SF-positive terminals. SP-positive dendrites in the rostral subnucleus were also in receipt of ChA…
author: Kawaja, Michael David
keywords: Biology, Anatomy
date published: 1989-01-01
date modified: 2026-07-27T21:56:01Z
provider: Western University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Western University

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property ID: Handle
value: https://hdl.handle.net/20.500.14721/21463