{"id":{"repo_id":"utswmed","oai_identifier":"oai:utswmed-ir.tdl.org:2152.5/9535"},"canonical_url":"https://search.dev.ndltd.org/etd/utswmed/oai:utswmed-ir.tdl.org:2152.5/9535","repository":{"repo_id":"utswmed","name":"University of Texas Southwestern Medical Center","base_url":"https://utswmed-ir.tdl.org/server/oai/request"},"display":{"title":"Quantitative Analysis of Integrin Trafficking and Focal Adhesion Turnover to Study Integration of Early Endocytic Trafficking, Signaling and Migration","abstract":"Pages 8-153 are misnumbered as pages 9-154.","abstract_html":"Pages 8-153 are misnumbered as pages 9-154.","abstract_has_math":false,"creators":["Lakoduk, Ashley Marie"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Danuser, Gaudenz","Ross, Theodora","Rosen, Michael K.","Schmid, Sandra"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-06-03T22:31:22Z","date_published":"2021-06-03T22:31:22Z","updated_at":"2026-07-24T05:52:36Z","subjects":["Acid-Base Equilibrium","Cell Movement","Endocytosis","Endosomal Sorting Complexes Required for Transport","Integrin beta1","Signal Transduction"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["1255189246"],"render_values":[{"text":"1255189246","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152.5/9535","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Danuser, Gaudenz","Ross, Theodora","Rosen, Michael K.","Schmid, Sandra"]},{"key":"dc:creator","label":"Author","values":["Lakoduk, Ashley Marie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-06-03T22:31:22Z","2019-05","2019-01-22","May 2019","2021-06-03T22:31:23Z"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Acid-Base Equilibrium","Cell Movement","Endocytosis","Endosomal Sorting Complexes Required for Transport","Integrin beta1","Signal Transduction"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2152.5/9535","1255189246"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Pages 8-153 are misnumbered as pages 9-154.","Early endocytic trafficking is critical for regulating both receptor presence at the plasma membrane and cellular signaling. Clathrin-mediated endocytosis (CME) constitutes a major route of selective receptor internalization, yet little is knowing about how this process is regulated both temporally and spatially. We chose to investigate focal adhesion (FA) and beta-1 integrin turnover as a model system to better understand the spatial, temporal, and cargo-specific regulation of early endocytic trafficking. Importantly, the dynamic turnover of integrins and their associated adhesion complexes through endocytic and recycling pathways has emerged as key mechanism for controlling cancer cell migration and invasion. However, current tools available to study integrin trafficking do not provide adequate spatial information or can induce artifacts. Here, we report the generation and characterization of a neutral and monovalent antibody-based probe to study beta-1 integrin trafficking in cells. Using this tool, we report a novel regulatory mechanism of integrin turnover by a lipid kinase, PIPKI, and discover a mutant p53-driven endosomal signaling nexus that regulates beta-1 integrin recycling and cancer cell invasion. This work demonstrates the importance of the reciprocal crosstalk between early endocytic trafficking and receptor signaling in regulating both normal and tumor cell function."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Quantitative Analysis of Integrin Trafficking and Focal Adhesion Turnover to Study Integration of Early Endocytic Trafficking, Signaling and Migration"]}]}],"canonical_facts":{"dc:contributor":["Danuser, Gaudenz","Ross, Theodora","Rosen, Michael K.","Schmid, Sandra"],"dc:creator":["Lakoduk, Ashley Marie"],"dc:date":["2021-06-03T22:31:22Z","2019-05","2019-01-22","May 2019","2021-06-03T22:31:23Z"],"dc:description":["Pages 8-153 are misnumbered as pages 9-154.","Early endocytic trafficking is critical for regulating both receptor presence at the plasma membrane and cellular signaling. Clathrin-mediated endocytosis (CME) constitutes a major route of selective receptor internalization, yet little is knowing about how this process is regulated both temporally and spatially. We chose to investigate focal adhesion (FA) and beta-1 integrin turnover as a model system to better understand the spatial, temporal, and cargo-specific regulation of early endocytic trafficking. Importantly, the dynamic turnover of integrins and their associated adhesion complexes through endocytic and recycling pathways has emerged as key mechanism for controlling cancer cell migration and invasion. However, current tools available to study integrin trafficking do not provide adequate spatial information or can induce artifacts. Here, we report the generation and characterization of a neutral and monovalent antibody-based probe to study beta-1 integrin trafficking in cells. Using this tool, we report a novel regulatory mechanism of integrin turnover by a lipid kinase, PIPKI, and discover a mutant p53-driven endosomal signaling nexus that regulates beta-1 integrin recycling and cancer cell invasion. This work demonstrates the importance of the reciprocal crosstalk between early endocytic trafficking and receptor signaling in regulating both normal and tumor cell function."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2152.5/9535","1255189246"],"dc:language":["en"],"dc:subject":["Acid-Base Equilibrium","Cell Movement","Endocytosis","Endosomal Sorting Complexes Required for Transport","Integrin beta1","Signal Transduction"],"dc:title":["Quantitative Analysis of Integrin Trafficking and Focal Adhesion Turnover to Study Integration of Early Endocytic Trafficking, Signaling and Migration"],"dc:type":["Thesis","text"]},"updated_at":"2026-07-24T05:52:36Z"}